Antibacterial peptide GLI23 derived from linear chicken beta-phylaxin4 (RL38) and preparation method thereof
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A defensin and antimicrobial peptide technology, applied in the field of antimicrobial peptide GLI23 derived from linear chicken β-defensin 4 and its preparation, can solve the problem of few natural antimicrobial peptides
Inactive Publication Date: 2012-03-21
NORTHEAST AGRICULTURAL UNIVERSITY
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[0003] At present, thousands of antimicrobial peptides have been extracted from various animals and plants, but there are few natural antimicrobial peptides used in the medical field as antibiotic substitutes
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Embodiment 1
[0030] Example 1: Design of antibacterial peptides
[0031] The amino acid sequence of linear chicken β-defensin 4 is:
[0032] Arg Tyr His Met Gln Cys Gly Tyr Arg Gly Thr Phe Cys Thr Pro
[0033] 1 5 10 15
[0034] Gly Lys Cys Pro Tyr Gly Asn Ala Tyr Leu Gly Leu Cys Arg Pro;
[0035] 20 25 30
[0036] Lys Tyr Ser Cys Cys Arg Trp Leu-NH 2
[0037] 35 38
[0038] By intercepting the 23 amino acids at the carboxyl terminal of linear chicken β-defensin 4 (RL38), two polypeptides with 4 positive charges and a hydrophobic value of -0.3 were obtained.
[0039] The obtained peptide GL23, its amino acid sequence is:
[0040] Gly Lys Cys Pro Tyr Gly Asn Ala Tyr Leu Gly Leu Cys Arg Pro
[0041] 1 6 10 15;
[0042] Lys Tyr Ser Cys Cys Arg Trp Leu--NH 2
[0043] 20 23
[0044] The first amino acid of GL23 is glycine to improve its stability.
[0045] Then replace the four cysteines in the GL23 peptide chain with isoleucine,
[0046] Obtain GLI23, whose amino acid se...
Embodiment 2
[0054] Synthesis and biological activity determination of antibacterial peptides
[0055] A peptide synthesizer is used to synthesize the above-mentioned antimicrobial peptides by a solid-phase synthesis method, and the biological activity of the antimicrobial peptides is identified and analyzed.
[0056] 2.1 Antibacterial activity
[0057] Prepare the peptide as a certain storage solution for use. The minimum bactericidal concentration of several antimicrobial peptides was determined by plate counting method. Using 10 mM sodium phosphate solution (pH 7.4) as the diluent, a series of gradient antimicrobial peptide solutions were sequentially configured using the double dilution method. Take 100μl of the above solution and place it in a 96-well cell culture plate, and then add an equal volume of the test bacteria solution (10 6 Pcs / ml) in each well. Incubate at 37°C for 2h. Then take an appropriate amount of the above liquid diluted and spread it on an agar plate, incubate at 37°C...
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Abstract
The invention provides an antibacterial peptide GLI23 derived from linear chicken beta-phylaxin4 (RL38) and a preparation method thereof. According to the invention, methods of fixed-point amino acid segment interception and amino acid replacement are adopted so as to simplify the linear chicken beta-phylaxin4 and obtain two polypeptides; the antibacterial and the hemolytic activities the polypeptides are measured; and the therapeutic index of the polypeptides are calculated so as to evaluate the selectivity of the polypeptides to cells. Found by researches, the intercepted peptide GLI23 and the peptide GLI23 after being subject to amino acid replacement have obvious bactericidal activity and obviously-decreased hemolytic activity compared with the chicken beta-phylaxin4, especially the GLI23 of which the therapeutic index is as high as 76.1. The method provided by the invention has the advantages that: under the circumstance that the bactericidal activity of the antibacterial peptideis not decreased, the hemolytic activity of the antibacterial peptide is decreased; the selectivity of the antibacterial peptide between bacterial cells and mammalian cells is improved; and the development potential of substituting for antibiotic is improved.
Description
Technical field [0001] The present invention belongs to the application field of agriculture, animal husbandry and veterinary medicine, and specifically relates to an antibacterial peptide GLI23 derived from linear chicken β-defensin 4 (RL38) and a preparation method thereof. Background technique [0002] Antibacterial peptides are active polypeptides with antibacterial effects widely present in organisms, and are the immune response products of biological non-specific defense systems. They have broad-spectrum antibacterial and antiviral, antifungal, antiparasitic and antitumor biological activities. Antimicrobial peptides have antibacterial or bactericidal effects due to their physical penetration on bacterial membranes, while bacteria and other microorganisms are difficult to change the cell membrane structure of their own phospholipid bilayers, which makes the antimicrobial peptides more resistant Greatly reduced. Therefore, the research of antimicrobial peptides has become a...
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