Antibacterial peptide VK-21 and application

A VK-21, antibacterial peptide technology, applied in the field of short peptides in the field of biotechnology, can solve the problems of low antibacterial activity, high toxicity and poor stability of antibacterial peptides, and achieve good antibacterial effect, strong antibacterial activity, Effect of low hemolytic activity

Active Publication Date: 2017-11-24
ZUNYI MEDICAL UNIVERSITY
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Quite a few natural antibacterial peptides have low antibacterial activity, poor stability, high toxicity, or cause hemolysis of eukaryotic cells; in addition, some AMPs have poor inhibitory effect on drug-resistant bacteria, which cannot meet the requirements of practical applications; By modifying natural AMPs or synthesizing brand-new artificial AMPs through rational design, some or even all of the above shortcomings can be improved to a large extent to meet different application requirements.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibacterial peptide VK-21 and application
  • Antibacterial peptide VK-21 and application
  • Antibacterial peptide VK-21 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Embodiment: The antimicrobial peptide VK-21 product of the present invention is the amino acid sequence in SEQ ID NO. 1, and its sequence information is Val-Lys-Arg-Lys-Lys-Lys-Pro-Gln-Ser-Trp-Lys-Thr- Trp-Trp-Thr-Lys-Trp-Trp-Thr-Lys-Lys.

[0023] Sequence characteristics: the length is 21, the type is amino acid sequence, the chain type is straight chain, and it is artificially synthesized.

[0024] The antimicrobial peptide VK-21 product of this example is synthesized by an automatic peptide synthesizer in accordance with conventional peptide solid phase, and the finally obtained antimicrobial peptide VK-21 is analyzed by high performance liquid chromatography, and its purity is ≥ 98%. Its concrete preparation steps are as follows:

[0025] 1) Resin swelling: Weigh 0.6g of 2-Chlorotrityl Chloride Resin resin with a degree of substitution of 0.4mmol / g, put the resin into a reaction tube, add DCM (15mL / g), and shake for 30min.

[0026] 2) Connect the first amino acid:...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an antibacterial peptide VK-21 in the technical field of biology. The amino acid sequence of the antibacterial peptide is Val-Lys-Arg-Lys-Lys-Lys-Pro-Gln-Ser-Trp-Lys-Thr-Trp-Trp-Thr-Lys-Trp-Trp-Thr-Lys-Lys. The antibacterial peptide has an obvious inhibiting effect on Escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and four methicillin-resistant staphylococcus aureus, has an excellent antibacterial effect on vibrio parahaemolyticus and vibrio harveyi frequently existing in aquatic products, has low hemolytic activity, good stability, strong antibacterial activity and efficient broad-spectrum bacteriostasis functions, and can be used as an antibiotic substitute.

Description

technical field [0001] The invention relates to a short peptide in the field of biotechnology, in particular to an antibacterial peptide VK-21 and its application. Background technique [0002] Aquatic products are a general term for animals or algae produced in oceans, rivers and lakes. Since there are many types of organisms in water bodies, and water is also a medium that is easy to breed pathogens, aquatic products often inevitably contain pathogenic bacteria, such as Vibrio parahaemolyticus and Vibrio harveyi are common pathogenic bacteria in aquatic products, which often cause illness or even mass death of aquatic products, causing huge economic losses to the aquaculture industry. At present, the commonly used antibacterial agents are basically chemical fungicides, but the use of chemical fungicides for inhibition will cause pollution to the ecological environment, and will inevitably remain in aquatic products, which will threaten the safety of humans and animals. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A23K20/147A23L33/18A61K38/16A61P31/04
CPCA23K20/147A23L33/18A23V2002/00A61K38/00C07K14/001A23V2250/55Y02A50/30
Inventor 杨愈丰杨传辉谢明峰柳敦耀夏嫱李均刘振勇张晓青向红英彭莉萍吕延成
Owner ZUNYI MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap