A volatile oil of atractylodes rhizome bacteriostatic air freshener and a preparation method thereof

Abstract

This invention provides an antibacterial air freshener made from Atractylodes lancea volatile oil and its preparation method, belonging to the field of daily consumer goods technology. It comprises the following components by volume fraction: 0.5%~1.5% Atractylodes lancea volatile oil, 1.5%~4.5% main solubilizer, 3%~8% co-solvent, 1%~3% fragrance, 30%~50% anhydrous ethanol, and the balance being water. Preferably, the main solubilizer is a composite solubilizer system composed of dodecyl dimethylamine oxide OB-2 and alkyl glycoside APG10, with a volume ratio of 1~3:1. The antibacterial air freshener made from Atractylodes lancea volatile oil of this invention is clear and transparent in appearance, and exhibits an antibacterial rate of greater than 99.99% against Staphylococcus aureus, Escherichia coli, and Candida albicans.

Description

Technical Field

[0001] This invention relates to the field of daily necessities technology, and in particular to an antibacterial air freshener made from Atractylodes lancea volatile oil and its preparation method. Background Technology

[0002] Air fresheners are a class of daily chemical products that improve indoor air quality by masking, decomposing, or neutralizing odor molecules in the air, or by inhibiting or killing bacteria, thereby enhancing indoor air comfort and safety. Functionally, they can be broadly categorized into two types: masking and purifying / antibacterial products. Traditional masking products prioritize fragrance over substance, making it difficult to fundamentally address air pollutant issues. In recent years, purifying / antibacterial air fresheners have become increasingly popular with consumers.

[0003] However, existing antibacterial liquid air fresheners either rely primarily on chemical agents (such as benzalkonium chloride), which can easily lead to bacterial resistance with long-term use, and excessively high concentrations may irritate mucous membranes, lacking the gentleness of natural ingredients. Others claim to be "natural" but are actually mainly composed of synthetic fragrances. Even those air freshener products that use natural plant-derived ingredients often require the addition of multiple plant essential oils from different sources, resulting in complex processes, inconsistent effectiveness, and high prices. Therefore, there is an urgent need to develop natural, safe, broad-spectrum antibacterial air fresheners that are cost-effective and reasonably priced.

[0004] Atractylodes lancea volatile oil is the main active ingredient in the rhizome of Atractylodes lancea. Its main components, atractylol, β-cineole, atractylodesin, and other active ingredients have synergistic antibacterial and antiviral effects, offering advantages such as natural safety and broad-spectrum antibacterial activity, which aligns with current consumer upgrades and health demands. However, although Atractylodes lancea volatile oil has significant antibacterial effects, its highly hydrophobic composition and strong odor limit its widespread application in indoor environments. Summary of the Invention

[0005] The purpose of this invention is to provide an antibacterial air freshener made from Atractylodes lancea volatile oil and its preparation method, so as to solve the above-mentioned technical problems.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides an antibacterial air freshener made from Atractylodes lancea volatile oil, comprising the following components by volume fraction: 0.5%~1.5% Atractylodes lancea volatile oil, 1.5%~4.5% main solubilizer, 3%~8% co-solvent, 1%~3% fragrance, 30%~50% anhydrous ethanol, and the balance is water.

[0007] Furthermore, it consists of components comprising the following volume fractions: 0.8%~1.2% Atractylodes lancea volatile oil, 2.0%~4.0% main solubilizer, 4%~6% co-solvent, 1.5%~2.5% fragrance, 35%~50% anhydrous ethanol, and the balance is water.

[0008] Furthermore, the main solubilizer is a composite solubilizer system, comprising dodecyl dimethylamine oxide OB-2 and alkyl glycoside APG10, wherein the volume ratio of dodecyl dimethylamine oxide OB-2 to alkyl glycoside APG10 is 1~3:1.

[0009] Furthermore, the co-solvent comprises propylene glycol and polyethylene glycol 400, wherein the volume ratio of propylene glycol to polyethylene glycol 400 is 1~3:2~5.

[0010] Furthermore, the fragrance contains one or more of gardenia, lemon, white tea, lavender, sandalwood, and jasmine.

[0011] This invention also provides a method for preparing an antibacterial air freshener using Atractylodes lancea volatile oil, comprising the following steps: 1) Add the volatile oil of Atractylodes lancea and the solubilizer to anhydrous ethanol and mix well to obtain the oil phase; 2) Add the composite solubilizer to water and mix well to obtain the aqueous phase; 3) Slowly pour the oil phase into the aqueous phase, then add the fragrance to obtain Atractylodes lancea volatile oil antibacterial air freshener.

[0012] The beneficial effects of this invention are: 1. This invention provides an antibacterial liquid air freshener based on Atractylodes lancea volatile oil, which has good antibacterial effect, pleasant aroma, simple production process and low cost.

[0013] 2. Compared with conventional formulations using Tween-80 as a solubilizer, the Atractylodes lancea volatile oil antibacterial air freshener prepared by the present invention using a composite solubilizing system composed of dodecyl dimethylamine oxide OB-2 and alkyl glycoside APG10 has a clear and transparent appearance and stronger antibacterial activity against Staphylococcus aureus, Escherichia coli and Candida albicans.

[0014] 3. Compared with the antibacterial activity of 1% Atractylodes lancea volatile oil alone, the antibacterial activity of the Atractylodes lancea volatile oil antibacterial air freshener provided by the present invention is significantly improved.

[0015] 4. The preparation method of the Atractylodes lancea volatile oil antibacterial air freshener provided by the present invention is simple and easy to operate, and can be quickly industrialized.

[0016] 5. This invention further processes Atractylodes lancea volatile oil into daily-use products, thereby increasing the added value of Atractylodes lancea and its volatile oil. Attached Figure Description

[0017] Figure 1The image shows the antibacterial effect of five different formulations of Atractylodes lancea volatile oil air fresheners on Escherichia coli. Figure 2 The antibacterial effect of five different formulations of Atractylodes lancea volatile oil air fresheners on Staphylococcus aureus is shown in the figure. Figure 3 The antibacterial effect of five different formulations of Atractylodes lancea volatile oil air fresheners on Candida albicans is shown in the figure. Figure 4 The diagram shows the inhibitory effect of the Atractylodes lancea volatile oil antibacterial air freshener obtained from the optimal formula (K6) on Staphylococcus aureus. Figure 5 The diagram shows the inhibitory effect of Atractylodes lancea volatile oil air freshener obtained from the optimal formula (K6) on Escherichia coli. Figure 6 The diagram shows the inhibitory effect of Atractylodes lancea volatile oil antibacterial air freshener obtained from the optimal formula (K6) on Candida albicans. Detailed Implementation

[0018] This invention provides an antibacterial air freshener made from Atractylodes lancea volatile oil, comprising the following components by volume fraction: 0.5%~1.5% Atractylodes lancea volatile oil, 1.5%~4.5% main solubilizer, 3%~8% co-solvent, 1%~3% fragrance, 30%~50% anhydrous ethanol, and the balance is water.

[0019] In this invention, the Atractylodes lancea volatile oil antibacterial air freshener is composed of components comprising the following volume fractions: 0.8%~1.2% Atractylodes lancea volatile oil, 2.0%~4.0% main solubilizer, 4%~6% co-solvent, 1.5%~2.5% fragrance, 30%~50% anhydrous ethanol, and the balance is water.

[0020] In this invention, the Atractylodes lancea volatile oil antibacterial air freshener is preferably composed of the following components by volume fraction: 1.0% Atractylodes lancea volatile oil, 3.0% main solubilizer, 5% co-solvent, 2% fragrance, 50% anhydrous ethanol, and the balance is water.

[0021] In this invention, the main solubilizer is a composite solubilizer system comprising dodecyl dimethylamine oxide OB-2 and alkyl glycoside APG10, wherein the volume ratio of dodecyl dimethylamine oxide OB-2 to alkyl glycoside APG10 is 1~3:1, preferably 2:1.

[0022] In this invention, the cosolvent comprises propylene glycol and polyethylene glycol 400, wherein the volume ratio of propylene glycol to polyethylene glycol 400 is 1~3:2~5, preferably 1~3:3~4, and more preferably 2:3.

[0023] In this invention, the fragrance contains one or more of gardenia, lemon, white tea, lavender, sandalwood, and jasmine.

[0024] This invention also provides a method for preparing an antibacterial air freshener using Atractylodes lancea volatile oil, comprising the following steps: 1) Add the volatile oil of Atractylodes lancea and the solubilizer to anhydrous ethanol and mix well to obtain the oil phase; 2) Add the composite solubilizer to water and mix well to obtain the aqueous phase; 3) Slowly pour the oil phase into the aqueous phase, then add the fragrance to obtain Atractylodes lancea volatile oil antibacterial air freshener.

[0025] The Atractylodes lancea volatile oil antibacterial air freshener of the present invention is suitable for masking or eliminating odors in home environments, office and commercial spaces, and public places, and inhibiting the growth of bacteria on object surfaces. It can inhibit the growth of Staphylococcus aureus, Escherichia coli, and Candida albicans.

[0026] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0027] Example

[0028] Atractylodes lancea volatile oil has high viscosity and a strong odor. In order to prepare a clear, transparent, and pleasant-smelling liquid air freshener, the inventors used the appearance, viscosity, and odor of the liquid air freshener as indicators to initially screen the types of main solubilizers, co-solvents, and fragrances in the formula, as well as the preliminary proportions of each component. The formulas of this invention are shown in Table 1 below.

[0029] Table 1 Formula

[0030] The preparation process of air fresheners: According to the formulation process, Atractylodes lancea volatile oil and solubilizer are added to anhydrous ethanol in sequence and stirred until homogeneous oil phase is obtained; the composite solubilizer is added to pure water and stirred until homogeneous aqueous phase is obtained; then the oil phase is slowly poured into the aqueous phase and stirred until homogeneous; finally, the fragrance is added and stirred until homogeneous to obtain Atractylodes lancea volatile oil antibacterial air freshener.

[0031] Antibacterial activity test: This invention provides an antibacterial air freshener made from Atractylodes lancea volatile oil. Its main advantage is its antibacterial effect. Therefore, the antibacterial activity of Atractylodes lancea volatile oil air fresheners prepared from five preliminary screening formulations was evaluated. The antibacterial activity of formulations TT, LA, TA, K5, and K6 against Escherichia coli, Staphylococcus aureus, and Candida albicans was determined using the filter paper disc method. The results showed that the antibacterial effect of different formulations against Escherichia coli was K6>TA>TT>K5>LA; against Staphylococcus aureus, K6>LA>K5>TA>TT; and against Candida albicans, K6>LA>K5>TA>TT. The results are shown in the table below. Figure 1 , Figure 2 and Figure 3 The diameter of the inhibition zone was measured, repeated three times, and the average value was taken (see Table 2). The results show that the K6 formulation has the best antibacterial effect, and the solution is clear, non-sticky, and has a low formulation cost. Therefore, the K6 formulation was ultimately selected as the base formulation for the liquid air freshener.

[0032] Table 2. Antibacterial zone results of air fresheners made from Atractylodes lancea volatile oil prepared with five different formulations.

[0033] Based on the appearance and antibacterial activity of liquid air fresheners, the basic formula of Atractylodes lancea volatile oil antibacterial air freshener was determined in the early stage. On this basis, orthogonal experiments were used to optimize its preparation process parameters.

[0034] Orthogonal experimental design

[0035] The process parameters were optimized using the L9(34) orthogonal experimental method. The effects of the amount of Atractylodes lancea volatile oil, compound solubilizer (OB-2:APG10 = 2:1) and anhydrous ethanol on the air freshener product were investigated using the appearance, viscosity and antibacterial effect of air freshener as evaluation indicators. The orthogonal experimental factor level table is shown in Table 3.

[0036] Table 3 Factor Level Table

[0037] Scoring Criteria

[0038] Each batch of air freshener was evaluated based on its appearance (Ya) and antibacterial activity (Yb). The appearance quality evaluation is divided into 5 levels, with scores ranging from 1 to 5, and the best score being 5. Therefore, the clarity and transparency score (Y1) and the viscosity score (Y2) are respectively their respective scores / best score (5). The antibacterial activity score against Escherichia coli (Y3) = measured inhibition zone (mm) / maximum inhibition zone (9.17 mm); the antibacterial activity score against Staphylococcus aureus (Y4) = measured inhibition zone (mm) / maximum inhibition zone (29.33 mm); and the antibacterial activity score against Candida albicans (Y5) = measured inhibition zone (mm) / maximum inhibition zone (16.33 mm). Based on the contribution of each factor to the air freshener product, the weight coefficients of the above indicators are 0.1, 0.3, 0.2, 0.2, and 0.2, respectively. Let Ya = (Y1 × 0.1 + Y2 × 0.3) × 100, Yb... = (Y3+Y4+Y5)×0.2×100, then the comprehensive score Ycomprehensive of the air fresheners prepared in each experimental batch is (Ya + Yb), with a full score of 100 points. The higher the score, the better the formula.

[0039] Experimental results

[0040] Based on the above scoring criteria, the results of each experiment were calculated and analyzed, as shown in Tables 4, 5, and 6. Tables 5 and 6 provide a clear analysis: for clarity and transparency, and viscosity, the order of importance of the factors is B (anhydrous ethanol) > A (volatile oil) > C (compound solubilizer), with factor B having a significant impact. For antibacterial effect, the order of importance of the factors is A (volatile oil) > B (anhydrous ethanol) > C (compound solubilizer), with factor A having the greatest impact. Both factors A and B are significant. Therefore, under the premise of ensuring antibacterial effect, and considering production costs and product appearance, the optimal formula A2B3C2 was ultimately selected, namely 1% Atractylodes lancea volatile oil, 50% anhydrous ethanol, and 3% compound solubilizer.

[0041] Table 4 Orthogonal experimental design and results

[0042] Table 5. Analysis of the influence of various factors on appearance and antibacterial effect

[0043] Table 6. Analysis of Variance of Orthogonal Experiments

[0044] F0.05(2,2) = 19.00; P < 0.05

[0045] Verification experiments and results

[0046] Three batches of Atractylodes lancea volatile oil antibacterial air fresheners were prepared in parallel according to the optimal formula (1% Atractylodes lancea volatile oil, 50% anhydrous ethanol, and 3% composite solubilizer (dodecyl dimethylamine oxide OB-2:APG10 = 2:1). Their antibacterial activity was determined, and the scores of each batch were evaluated. The results are shown in Table 6. The Atractylodes lancea volatile oil air freshener prepared using the optimal formula had an average comprehensive score of 97.93 and an RSD of 0.66%, indicating that the method has high repeatability and good stability.

[0047] Table 7. Validation Experiment Data and Results

[0048] Evaluation of the antibacterial activity of Atractylodes lancea volatile oil in air freshener

[0049] Three batches of Atractylodes lancea volatile oil antibacterial air fresheners were prepared using the optimal formula. Their antibacterial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans was determined. Results are shown below. Figure 4 , Figure 5 and Figure 6 The average inhibition zone against Staphylococcus aureus was 28.5 mm, which was 13.5 mm larger than that of 1% Atractylodes lancea volatile oil; the average inhibition zone against Escherichia coli was 8.45 mm, and the average inhibition zone against Candida albicans was 20.28 mm. These results indicate that the Atractylodes lancea volatile oil antibacterial air freshener prepared using the preferred preparation process has clear antibacterial activity.

[0050] The antibacterial activity of three batches of Atractylodes lancea volatile oil antibacterial air fresheners prepared with the optimal formula was further evaluated according to the suspension quantitative method included in QB / T 2738-2023 "Evaluation Method for Antibacterial and Bacteriostatic Activity of Daily Chemical Products". The results are shown in Tables 8 and 9. After 2 minutes of action, the antibacterial air fresheners of Atractylodes lancea volatile oil had an inhibition rate of more than 99.99% against Escherichia coli, Staphylococcus aureus and Candida albicans, and had a strong antibacterial effect.

[0051] Table 8 Results of quantitative antibacterial test of suspension

[0052] Table 9 Evaluation of antibacterial effect

[0053] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A volatile oil-based antibacterial air freshener containing Atractylodes lancea, characterized in that, It consists of components including the following volume fractions: 0.5%~1.5% Atractylodes lancea volatile oil, 1.5%~4.5% main solubilizer, 3%~8% co-solvent, 1%~3% fragrance, 30%~50% anhydrous ethanol, and the balance is water.

2. The Atractylodes lancea volatile oil antibacterial air freshener according to claim 1, characterized in that, It consists of components including the following volume fractions: 0.8%~1.2% Atractylodes lancea volatile oil, 2.0%~4.0% main solubilizer, 4%~6% co-solvent, 1.5%~2.5% fragrance, 35%~50% anhydrous ethanol, and the balance is water.

3. The Atractylodes lancea volatile oil antibacterial air freshener according to claim 1 or 2, characterized in that, The main solubilizer is a composite solubilizer system, comprising dodecyl dimethylamine oxide OB-2 and alkyl glycoside APG10, wherein the volume ratio of dodecyl dimethylamine oxide OB-2 to alkyl glycoside APG10 is 1~3:

1.

4. The Atractylodes lancea volatile oil antibacterial air freshener according to claim 3, characterized in that, The cosolvent comprises propylene glycol and polyethylene glycol 400, wherein the volume ratio of propylene glycol to polyethylene glycol 400 is 1~3:2~5.

5. The Atractylodes lancea volatile oil antibacterial air freshener according to claim 1 or 2, characterized in that, The fragrance contains one or more of gardenia, lemon, white tea, lavender, sandalwood, and jasmine.

6. The method for preparing the Atractylodes lancea volatile oil antibacterial air freshener according to any one of claims 1 to 5, characterized in that, Includes the following steps: 1) Add the volatile oil of Atractylodes lancea and the solubilizer to anhydrous ethanol and mix well to obtain the oil phase; 2) Add the composite solubilizer to water and mix well to obtain the aqueous phase; 3) Slowly pour the oil phase into the aqueous phase, then add the fragrance to obtain Atractylodes lancea volatile oil antibacterial air freshener.

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