Transformed body HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus and preparation method and application of transformed body

A technology of HC-15 and Qinghuan sea snake, which is applied in the direction of resisting vector-borne diseases, medical preparations containing active ingredients, peptides, etc., to achieve the effect of small molecular weight, low hemolytic activity, and simple preparation method

Active Publication Date: 2014-03-26
SHANDONG INT BIOTECH PARK DEV +2
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, there is no report on the antimicrobial peptides of the cathelicidins family of Qinghuan sea snakes

Method used

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  • Transformed body HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus and preparation method and application of transformed body

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 prepares the transformed body HC-15 of antimicrobial peptide Hc-CATH

[0016] I. The preparation method of HC-15: according to the amino acid sequence of the Qinghuan sea snake antimicrobial peptide Hc-CATH, the modified body HC-15 of Hc-CATH is designed by molecular transformation method. The full sequence was synthesized by an automatic peptide synthesizer (433A, Applied Biosystems), and desalted by HPLC reverse-phase column chromatography.

[0017] II. Molecular weight was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF).

[0018] III. The purified HC-15 was identified for its purity by high-performance liquid chromatography (HPLC), and its molecular weight was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF). Amino acid sequence structure.

[0019] The measurement result is:

[0020] HC-15 is a variant of the Qinghuan sea snake antimicrobial pe...

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Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to a transformed body antimicrobial peptide HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus and a preparation method and application of the transformed body. The transformed body antimicrobial peptide is the transformed body HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus, is linear chain polypeptide and comprises 15 amino acid residues, the tail end of C- is amidated, the molecular weight is 1974.46Da, and the isoelectric point is 12.61. The transformed body antimicrobial peptide HC-15 has high-potency antimicrobial activity; furthermore, the transformed body antimicrobial peptide HC-15 has beneficial characteristics of small molecular weight, simple structure, low hemolytic activity and simple preparation method.

Description

technical field [0001] The invention relates to an antimicrobial peptide and its preparation method and application, in particular to an antimicrobial peptide HC-15, a modified antibacterial peptide of Hydrophis cyanocinctus (Hydrophis cyanocinctus), and its preparation method and application. Background technique [0002] Cathelicidins are a family of multifunctional antimicrobial peptides that are currently found in mammals, birds, reptiles, amphibians and fish. Cathelicidins have broad-spectrum and high-efficiency antimicrobial activity, not only have very strong bactericidal activity against Gram-positive bacteria, Gram-negative bacteria, some fungi and viruses, but also have effects on many clinical drug-resistant bacteria. In addition, cathelicidins also have many other biological activities, such as chemotaxis to various immune cells, induction of mast cell degranulation and histamine release, regulation of macrophage transcription, promotion of wound healing, inducti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/00
CPCY02A50/30
Inventor 王义鹏张淑敏谢则平
Owner SHANDONG INT BIOTECH PARK DEV
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