Pit-hole composite micro-nano-structure polysaccharide microsphere and preparation method

A micro-nano structure and polysaccharide technology, applied in the preparation of microspheres, microcapsule preparations, pharmaceutical formulations, etc., can solve the problems of difficult to control microscopic morphology, single structure, large particle size distribution, etc., and achieve good physical and chemical properties, The effect of uniform particle size distribution and high porosity

Active Publication Date: 2019-03-26
WENZHOU INST OF BIOMATERIALS & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, the microspheres prepared by the above methods have the problems of single structure, difficult control of microscopic morphology, and excessive particle size distribution.

Method used

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  • Pit-hole composite micro-nano-structure polysaccharide microsphere and preparation method
  • Pit-hole composite micro-nano-structure polysaccharide microsphere and preparation method
  • Pit-hole composite micro-nano-structure polysaccharide microsphere and preparation method

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Embodiment 1

[0044] The preparation method of pit-hole composite micro-nano structure polysaccharide microspheres comprises the following steps:

[0045] (1) The mass ratio is 1:2:2, the degree of substitution: ≥80%, carboxymethyl chitosan with a viscosity of 10mPa.s, a weight-average molecular weight of 100,000 sodium hyaluronate and a viscosity of 100mPa.s Sodium alginate is dissolved in ultrapure water to make a polysaccharide aqueous solution with a mass concentration of 2%;

[0046] (2) Add the emulsifier obtained by mixing Span 85 and Tween-80 with a mass ratio of 1:1 into liquid paraffin so that the mass fraction of the emulsifier is 0.4%, stir and dissolve at 40°C to obtain emulsifier-containing Oil phase; add the polysaccharide aqueous solution dropwise to the oil phase containing the emulsifier at room temperature, stir and emulsify at 500rpm, and the stirring time is 4h to obtain a uniform emulsion; the mass ratio of the oil phase containing the emulsifier to the polysaccharide ...

Embodiment 2

[0053] The preparation method of pit-hole composite micro-nano structure polysaccharide microspheres comprises the following steps:

[0054] (1) Dissolving carboxymethyl cellulose with a mass ratio of 1:1:1 with a viscosity of 4500mPa.s, carboxymethyl dextran with a weight average molecular weight of 100,000, and sodium hyaluronate with a weight average molecular weight of 500,000 in ultrapure In water, it is made into a polysaccharide aqueous solution with a mass concentration of 10%;

[0055] (2) Add the emulsifier Span 80 into the soybean oil so that the mass fraction of the emulsifier is 5%, stir and dissolve at 80°C to obtain an oil phase containing the emulsifier; then add the polysaccharide aqueous solution dropwise at room temperature Into the oil phase containing emulsifier, stir and emulsify at 800rpm, and stir for 1h to obtain a uniform emulsion; the mass ratio of oil phase containing emulsifier to polysaccharide aqueous solution is 3:1;

[0056] (3) ZnCl 2 Dissol...

Embodiment 3

[0062] The preparation method of pit-hole composite micro-nano structure polysaccharide microspheres comprises the following steps:

[0063] (1) Carboxymethyl dextran with a weight average molecular weight of 80,000, a weight average molecular weight of 400,000 sodium hyaluronate, and a viscosity of 150mpa.s sodium alginate dissolved in ultrapure water with a mass ratio of 2:1:1, It is made into a polysaccharide aqueous solution with a mass concentration of 6%;

[0064] (2) The emulsifier obtained by mixing Span 60 and Triton X-100 with a mass ratio of 4:1 is added to liquid paraffin and normal hexane with a mass ratio of 5:1, so that the mass fraction of the emulsifier is 1%. ℃, stirring and dissolving to obtain an oil phase containing an emulsifier; then add the polysaccharide aqueous solution dropwise to the oil phase containing an emulsifier at room temperature, stir and emulsify at 600 rpm for 2 hours, and obtain a uniform emulsion; The mass ratio of oil phase and polysa...

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Abstract

The invention discloses a pit-hole composite micro-nano-structure polysaccharide microsphere and a preparation method. The preparation method comprises the following steps: (1) preparing a polysaccharide water solution; (2) adding an emulsifying agent into an oil phase so as to prepare the oil phase containing the emulsifying agent, dropwise adding the polysaccharide water solution into the oil phase containing the emulsifying agent, and stirring for emulsification, so as to obtain a uniform emulsion; (3) dissolving a polyvalent metal cross-linking agent into ultrapure water to prepare a cross-linking agent water solution, dropwise adding the cross-linking agent water solution into the uniform emulsion; and (4) adding n-hexane or petroleum ether into liquid obtained in the step (3), standing until the solution is layered, pouring out an upper oil phase layer, remaining a water phase, cleaning the water phase, and carrying out freeze-drying, so as to obtain the pit-hole composite micro-nano-structure polysaccharide microsphere. The surface of the microsphere is provided with nano-scale pits, and nano-scale fine holes are formed in the surfaces of the pits; the microsphere is uniformin particle size distribution, high in porosity and water absorption rate and strong in adsorbability; and the microsphere has good biocompatibility, and hemostasis can be effectively finished within20s-50s.

Description

technical field [0001] The invention relates to a pit-hole composite micro-nano structure polysaccharide microsphere and a preparation method thereof, belonging to the technical field of polymer material preparation. Background technique [0002] Natural polymer polysaccharides have the advantages of rich resources, green and pollution-free, good biocompatibility, and biodegradability. Polysaccharide microspheres with a porous structure are used in hemostasis, drug loading, tissue engineering scaffolds, protein purification, and metal ion adsorption and separation. and other fields have great applications. [0003] The patent application number 201610011690.X reports a preparation method of core-shell composite porous microspheres, which uses sodium alginate, chitosan, cellulose, gelatin, etc. as shell materials, and chitosan porous microspheres , cellulose porous microspheres, polylactic acid porous microspheres, gelatin porous microspheres, etc. as the core, through the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08L5/04C08L1/28C08L5/02C08L91/06C08L91/00C08K3/16B01J13/14C08J3/24A61L24/08A61L24/00
CPCA61L24/001A61L24/0036A61L24/0042A61L24/08A61L2400/04B01J13/14C08J3/246C08K2003/162C08K2003/168C08L1/286C08L5/02C08L5/04C08L5/08C08L91/06C08K3/16C08L91/00
Inventor 石长灿席光辉冯亚凯陈浩刘雯杨啸
Owner WENZHOU INST OF BIOMATERIALS & ENG
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