Application of pit-hole composite micro-nano-structure polysaccharide micro-sphere to preparation of haemostatic wound dressing

A technology of micro-nano structure and hemostatic dressing, which is applied in application, surgical adhesives, medical science, etc., can solve the problems of difficult control of microscopic morphology, single structure, large particle size distribution, etc., and achieve good physical and chemical properties and particle size distribution. Uniform, high porosity effect

Active Publication Date: 2019-02-22
安徽中科迈德医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In short, the microspheres prepared by the above methods have the problems of single structure, difficult control of microscopic morphology, and excessive particle size distribution.

Method used

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  • Application of pit-hole composite micro-nano-structure polysaccharide micro-sphere to preparation of haemostatic wound dressing
  • Application of pit-hole composite micro-nano-structure polysaccharide micro-sphere to preparation of haemostatic wound dressing
  • Application of pit-hole composite micro-nano-structure polysaccharide micro-sphere to preparation of haemostatic wound dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of pit-hole composite micro-nano structure polysaccharide microspheres comprises the following steps:

[0044] (1) The mass ratio is 1:2:2, the degree of substitution: ≥80%, carboxymethyl chitosan with a viscosity of 10mPa.s, a weight-average molecular weight of 100,000 sodium hyaluronate and a viscosity of 100mPa.s Sodium alginate is dissolved in ultrapure water to make a polysaccharide aqueous solution with a mass concentration of 2%;

[0045] (2) Add the emulsifier obtained by mixing Span 85 and Tween-80 with a mass ratio of 1:1 into liquid paraffin so that the mass fraction of the emulsifier is 0.4%, stir and dissolve at 40°C to obtain emulsifier-containing Oil phase; add the polysaccharide aqueous solution dropwise to the oil phase containing the emulsifier at room temperature, stir and emulsify at 500rpm, and the stirring time is 4h to obtain a uniform emulsion; the mass ratio of the oil phase containing the emulsifier to the polysaccharide ...

Embodiment 2

[0052] The preparation method of pit-hole composite micro-nano structure polysaccharide microspheres comprises the following steps:

[0053] (1) Dissolving carboxymethyl cellulose with a mass ratio of 1:1:1 with a viscosity of 4500mPa.s, carboxymethyl dextran with a weight average molecular weight of 100,000, and sodium hyaluronate with a weight average molecular weight of 500,000 in ultrapure In water, it is made into a polysaccharide aqueous solution with a mass concentration of 10%;

[0054] (2) Add the emulsifier Span 80 into the soybean oil so that the mass fraction of the emulsifier is 5%, stir and dissolve at 80°C to obtain an oil phase containing the emulsifier; then add the polysaccharide aqueous solution dropwise at room temperature Into the oil phase containing emulsifier, stir and emulsify at 800rpm, and stir for 1h to obtain a uniform emulsion; the mass ratio of oil phase containing emulsifier to polysaccharide aqueous solution is 3:1;

[0055] (3) ZnCl 2 Dissol...

Embodiment 3

[0061] The preparation method of pit-hole composite micro-nano structure polysaccharide microspheres comprises the following steps:

[0062] (1) Carboxymethyl dextran with a weight average molecular weight of 80,000, a weight average molecular weight of 400,000 sodium hyaluronate, and a viscosity of 150mpa.s sodium alginate dissolved in ultrapure water with a mass ratio of 2:1:1, It is made into a polysaccharide aqueous solution with a mass concentration of 6%;

[0063] (2) The emulsifier obtained by mixing Span 60 and Triton X-100 with a mass ratio of 4:1 is added to liquid paraffin and normal hexane with a mass ratio of 5:1, so that the mass fraction of the emulsifier is 1%. ℃, stirring and dissolving to obtain an oil phase containing an emulsifier; then add the polysaccharide aqueous solution dropwise to the oil phase containing an emulsifier at room temperature, stir and emulsify at 600 rpm for 2 hours, and obtain a uniform emulsion; The mass ratio of oil phase and polysa...

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Abstract

The invention discloses an application of a pit-hole composite micro-nano-structure polysaccharide micro-sphere to preparation of haemostatic wound dressing. According to GB / T16886.5-2003 in-vitro cytotoxicity judgment standards, acquired pit-hole composite micro-nano-structure polysaccharide micro-sphere extracting solution has 0-grade cytotoxicity under the concentration of 10-40 micrograms / L and shows good biocompatibility, bleeding can be effectively stopped within 20s-50s, good physical and chemical property is achieved, hemolysis ratio is smaller than 5%, national standards are met, themicro-sphere has an instant haemostatic function for a liver injury wound surface (haemostatic time is shorter than 5 seconds), haemostatic materials are completely degraded 72 hours after, and any residue is omitted. The pit-hole composite micro-nano-structure polysaccharide micro-sphere can stop bleeding within 20 seconds when being used for excessive bleeding of a femoral artery, surgical woundsurface recovers normal one week after, and any haemostatic micro-sphere sample residues are omitted.

Description

technical field [0001] The invention relates to the use of polysaccharide microspheres with pit-hole composite micro-nano structure in the preparation of hemostatic dressings, and belongs to the technical field of polymer material preparation. Background technique [0002] Natural polymer polysaccharides have the advantages of rich resources, green and pollution-free, good biocompatibility, and biodegradability. Polysaccharide microspheres with a porous structure are used in hemostasis, drug loading, tissue engineering scaffolds, protein purification, and metal ion adsorption and separation. and other fields have great applications. [0003] The patent application number 201610011690.X reports a preparation method of core-shell composite porous microspheres, which uses sodium alginate, chitosan, cellulose, gelatin, etc. as shell materials, and chitosan porous microspheres , cellulose porous microspheres, polylactic acid porous microspheres, gelatin porous microspheres, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/0036A61L24/0042A61L24/0094A61L2400/04
Inventor 石长灿席光辉冯亚凯陈浩刘雯杨啸
Owner 安徽中科迈德医疗科技有限公司
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