Nano drug of photoinduced release of nitric oxide anti-biofilm, and preparation and application method

A technology of nitric oxide and light-induced release, applied in the field of biomedical engineering materials, can solve the problems of difficult antibacterial strategy and short half-life, and achieve the effect of high-efficiency photothermal sterilization

Active Publication Date: 2021-08-20
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the free radical nature of NO, its relatively short half-life in the biological environment makes it difficult to be directly applied to the antibacterial strategy for dissipating biofilms.

Method used

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  • Nano drug of photoinduced release of nitric oxide anti-biofilm, and preparation and application method
  • Nano drug of photoinduced release of nitric oxide anti-biofilm, and preparation and application method
  • Nano drug of photoinduced release of nitric oxide anti-biofilm, and preparation and application method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation method of the nanometer material of photoinduced release NO antibiofilm, comprises the steps:

[0057] The first step is to synthesize anti-biofilm polydopamine@gold hybrid nanomaterials (PDG@Au)

[0058] Use a graduated cylinder to measure 200 mL of deionized water into a 500 mL beaker, disperse at a high speed at 2800 rpm, and then add 242 mg of Tris and 256 mg of acetylglucosamine into the beaker in sequence. After 5 minutes, 220 mg of dopamine hydrochloride was added to the above solution to initiate the reaction, and the reaction was carried out at room temperature and 2800 rpm under high-speed dispersion conditions for 1 hour. After the reaction, the reaction solution was transferred to a 50 mL centrifuge tube, centrifuged at 10,000 rpm, washed with deionized water, and freeze-dried to obtain PDNG.

[0059] Disperse the obtained PDNG in absolute ethanol at a more suitable concentration, and then ultrasonically disperse evenly, take an appropriate ...

Embodiment 2

[0106] Change the quality of acetylglucosamine and dopamine hydrochloride in the first step from 256mg and 220mg to 256mg and 200mg, the condensation reflux time is changed from 1h to 0.5h, and the magnetic stirring time is changed from 1h to 0.5h; The concentration of (AM-b-AMPBA) was changed from 0.7μg / mL to 0.4μg / mL, and the magnetic stirring time was changed from 8h to 6h; the TCF concentration in the third step was changed from 20mg / mL to 10mg / mL, and the magnetic stirring time Change from 72h to 60h; other preparation conditions remain unchanged, and prepare nanomaterials that photorelease NO to resist biofilm.

Embodiment 3

[0108] Change the quality of the acetylglucosamine and dopamine hydrochloride of the first step into 256mg and 240mg by 256mg and 220mg, the condensation reflux time is changed into 2h by 1h, the magnetic stirring time 1h is changed into 2h; -b-AMPBA) concentration was changed from 0.7μg / mL to 1.0μg / mL, and the magnetic stirring time was changed from 8h to 10h; the TCF concentration in the third step was changed from 20mg / mL to 30mg / mL, and the magnetic stirring time was changed from 72h Change it to 84h; other preparation conditions remain unchanged, and prepare nanomaterials that resist biofilm by photoreleasing NO.

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Abstract

The invention relates to a nano drug of photoinduced release of nitric oxide anti-biofilm, and a preparation and application method. A synthesis method includes the following steps: (1) performing oxidation co-deposition on acetylglucosamine and dopamine, obtaining glucosamine through deacetylation, and preparing a cationic monosaccharide modified polydopamine nanomaterial (PDG); (2) using the reducibility of an orthophthalic bisphenol structure on the PDG to in situ reduce HAuCl4 into elemental gold nanoparticles, and synthesizing a glucosamine modified polydopamine@gold hybrid nanomaterial (PDG@Au); (3) blending an NO donor TCF and the PDG@Au in a glass sample bottle, and obtaining a PDG@Au-NO nanomaterial through magnetic stirring and centrifugal washing; and (4) modifying diblock copolymer p(AM-b-APBA) prepared from monomer N-3-acrylamide phenyl boric acid (APBA) and monomer4-acryloyl morpholine (AMP) through RAFT polymerization on a surface of the PDG@Au, and preparing PDG@Au/PBA. The nanomaterial obtained by the synthesis method has a good antibacterial effect and an effect of dissipating biofilm.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and relates to a nano-medicine for photoinduced nitric oxide anti-biofilm and a preparation and use method. Background technique [0002] Due to the needs of bacterial evolution and survival and the abuse of antibiotics, the problem of bacterial resistance has become increasingly serious. According to statistics, 80% of bacteria exist in the form of biofilm, which shows strong resistance no matter in harsh environment or in the presence of antibiotics. In order to cope with the non-negligible crisis of drug-resistant bacteria, on the one hand, it is necessary to reduce the amount of antibiotics and avoid the abuse of antibiotics, and on the other hand, it is urgent to develop new treatments for bacterial biofilm infections. [0003] As a gas signal molecule, NO (Nitric oxide) can induce the dissipation of bacterial biofilm by regulating the intracellular second messenger cyclic gu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K33/00A61K47/52A61K47/59A61K47/54A61K47/58A61P31/04A61P17/02A61P29/00C08G73/06C08F293/00
CPCA61K41/0052A61K41/0042A61K33/00A61K41/0057A61K47/52A61K47/59A61K47/549A61K47/58A61P31/04A61P17/02A61P29/00C08G73/0672C08F293/00A61K2300/00Y02A50/20
Inventor 王腾蛟黄维李鹏王雷唐奕章王坤张善宇
Owner NORTHWESTERN POLYTECHNICAL UNIV
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