Sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and method for preparing same

A technology of polytetrafluoroethylene and sulfonated silk fibroin, which is applied in pharmaceutical formulations, coatings, drug delivery, etc., can solve problems such as fibroin film chapping and affecting the performance of artificial blood vessels, achieve good anastomosis, meet clinical application, The effect of low hemolysis rate

Inactive Publication Date: 2016-06-15
闫玉生
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  • Application Information

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Problems solved by technology

The results showed that the sulfonic acid groups were successfully introduced into the inner surface of ePTFE. However, the silk fibroin film on the inner surface of the artificial blood vessel is prone to a large number of cracks, which will inevitably affect the performance of the artificial blood vessel in clinical application. Its compatibility with blood and tissue also needs to be further improved

Method used

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  • Sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and method for preparing same
  • Sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and method for preparing same
  • Sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and method for preparing same

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preparation example Construction

[0033] see figure 1 , a method for preparing a sulfonated silk fibroin membrane-modified polytetrafluoroethylene artificial blood vessel according to one embodiment, comprising the following steps.

[0034] Step S100: subjecting the polytetrafluoroethylene (PTFE) artificial blood vessel to low-temperature plasma surface treatment to obtain a pretreated polytetrafluoroethylene artificial blood vessel.

[0035] Since PTFE is an inert polymer with low surface energy, if silk fibroin is directly coated on the surface of PTFE, the adhesion stability is poor. Low-temperature plasma surface treatment of PTFE artificial blood vessels can increase the surface free energy of PTFE, make silk fibroin easy to adhere to the surface of PTFE, and improve the stability of adhesion of silk fibroin on the surface of PTFE.

[0036] In one embodiment, the processing gas for the low-temperature plasma surface treatment is argon, and the discharge power is 40-80W. A large number of experimental st...

Embodiment 1

[0057] (1) The ePTFE artificial blood vessel with an inner diameter of 4mm is subjected to low-temperature plasma surface treatment to obtain a pretreated polytetrafluoroethylene artificial blood vessel; the treatment gas for low-temperature plasma surface treatment is argon, the fixed discharge voltage pressure is 70Pa, and the discharge power It is 80W, and the discharge time is 10min. The pretreated polytetrafluoroethylene artificial blood vessel was placed in the air for 10 minutes.

[0058] (2) prepare silk fibroin solution: the molar concentration of silk is 0.5% Na 2 CO 3 Boil in the solution for 120min, wash and dry with water, add CaCl with a molar ratio of 1:8:2 2 、H 2 A ternary solvent of O and ethanol was dissolved in a water bath at 80°C for 24 hours, then dialyzed in distilled water and deionized water for 3 days, and suction filtered to obtain a silk fibroin solution.

[0059] (3) Place the pretreated polytetrafluoroethylene artificial blood vessel verticall...

Embodiment 2

[0062] The difference between embodiment 2 and embodiment 1 is:

[0063] The inner diameter of the ePTFE artificial blood vessel in Example 2 is 6mm.

[0064] The discharge power of the low-temperature plasma surface treatment in step (1) is 60W.

[0065] The concentration of the silk fibroin solution in step (3) is 1.6 mg / mL.

[0066] The discharge power of the low-temperature plasma surface sulfonation treatment in step (4) is 90W.

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Abstract

The invention relates to a sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and a method for preparing the same.The method includes steps of carrying out low-temperature plasma surface pre-treatment on a polytetrafluoroethylene artificial blood vessel, vertically or obliquely arranging the polytetrafluoroethylene artificial blood vessel, sealing one end of the polytetrafluoroethylene artificial blood vessel, forming an opening in the other end of the polytetrafluoroethylene artificial blood vessel, keeping the opening upwards, injecting 0.5-2.0 mg/mL silk fibroin solution into the polytetrafluoroethylene artificial blood vessel from the opening, evaporating the silk fibroin solution and coating the silk fibroin solution in the polytetrafluoroethylene artificial blood vessel; turning the polytetrafluoroethylene artificial blood vessel over, keeping the opening upwards, injecting 0.5-2.0 mg/mL silk fibroin solution into the polytetrafluoroethylene artificial blood vessel from the opening, evaporating the silk fibroin solution and coating the silk fibroin solution in the polytetrafluoroethylene artificial blood vessel to obtain a silk fibroin film modified polytetrafluoroethylene artificial blood vessel, and carrying out low-temperature plasma surface sulfonation treatment on the silk fibroin film modified polytetrafluoroethylene artificial blood vessel to obtain the sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel.The sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and the method have the advantages that the sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel prepared by the aid of the method is good in uniformity and excellent in blood and tissue compatibility and rarely has chap and crack.

Description

technical field [0001] The invention relates to the field of preparation of polytetrafluoroethylene artificial blood vessels, in particular to a sulfonated silk fibroin membrane modified polytetrafluoroethylene artificial blood vessel and a preparation method thereof. Background technique [0002] The development of artificial blood vessels began in the early 20th century. Scholars from various countries have successively used materials such as metal, glass, polyethylene, and silicone rubber to make vascular substitutes. Therefore, it has not been widely used clinically. Since Voorhees invented the artificial blood vessel with mesh Vinylon (Vinylon "N") in 1952, researchers from various countries have successively developed a series of artificial blood vessels with pores using various materials and processing methods and used them in animal experiments and clinics. Such as polyester (dacron) and so on. However, the research results show that artificial blood vessels made o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34
CPCA61L27/34A61L2300/606A61L2400/18A61L2420/02
Inventor 闫玉生李少彬张家庆李辉黄和锡
Owner 闫玉生
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