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Pseudomonas strain and application thereof

A technology of Pseudomonas and strains, which is applied in the field of agricultural microorganisms and biological control, can solve the problems of negative impact on human health in the ecological environment, environmental pollution by chemical pesticides, and drug resistance of pathogens, and achieve remarkable control effects, reduce pollution, and promote The effect of plant growth

Active Publication Date: 2020-12-11
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in agricultural production, the method of preventing plant soil-borne diseases mainly relies on traditional chemical control, but chemical pesticides pollute the environment and produce pesticide residues, which have a negative impact on the ecological environment and human health, and long-term use is easy to make pathogens resistant to pesticides

Method used

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  • Pseudomonas strain and application thereof
  • Pseudomonas strain and application thereof
  • Pseudomonas strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and Identification of Pseudomonas Strains

[0030] (1) Separation and purification

[0031] Beef extract-peptone agar medium: 3g beef extract, 10g peptone, 10g sodium chloride, 15g agar, 1000mL deionized water, pH 7.4, sterilized at 121°C for 30min.

[0032] The inventor collected plant rhizosphere soil samples from Changsha City, Hunan Province, and after natural air-drying, weighed 10 g of samples and dissolved them in 90 mL of sterile water to make a soil suspension. Then use 10-fold gradient dilution with sterile water, draw 10 -3 and 10 -4 100 μL of the soil suspension was evenly spread on the beef extract peptone medium plate; after the above plate was placed in the incubator at 30°C for 36h-48h, different colonies were picked according to the colony shape, color and size, and placed in the new Purify on the beef extract peptone medium plate until pure colonies are obtained and numbered for preservation.

[0033] (2) Morphological and phys...

Embodiment 2

[0042] The preparation of embodiment 2 microbial bacterial agents

[0043] Beef extract-peptone broth (NB): 3g beef extract, 10g peptone, 10g sodium chloride, 1000mL deionized water, pH 7.4, sterilized at 121°C for 30min.

[0044] (1) Cultivation of slant seeds: Aseptically streak the thalli of Pseudomonas strain GC-7 onto the slant of beef extract peptone agar medium, cultivate at 30° C. for 24 hours to obtain slant seeds;

[0045] (2) Liquid seed culture: pick an activated single colony from the slope and inoculate it in NB medium for culture, 180r / min, 30°C, and the culture time is 24h to obtain the seed culture solution;

[0046] (3) Fermentation culture: insert the seed culture liquid into NB culture medium according to the inoculation amount of 5%, 30 ℃, the rotating speed is 180r / min, and the fermentation time is 48h;

[0047](4) Preparation of fermented liquid: collect the fermented liquid fermented and cultivated, and prepare it into microbial liquid or solid microbi...

Embodiment 3

[0048] Example 3: Activity Detection of Pseudomonas Strain GC-7 Lethal Plant Nematodes

[0049] Potato dextrose agar medium (PDA): 200g potato, 20g glucose, 15g-20g agar, 1000mL water.

[0050] 1. Preparation of fermentation supernatant: Pseudomonas bacterial strain GC-7 is fermented by the method of embodiment 2, collects the fermentation culture of fermentation 48h, and the centrifugation rate is 10000r / min, and the time is 10min, then take the upper The clear liquid is ready for use.

[0051] 2. Preparation of nematode suspension: D. destructor potato used in this example was preserved by the Plant Nematode Laboratory of Hunan Agricultural University. Before culturing, inoculate Fusarium half naked on a PDA plate, and incubate at 25°C for 7-10 days. After the mycelia cover the entire plate, insert 100 μL of D. destructor suspension under aseptic conditions, and incubate at 25°C for 7-10 days. After the half-naked Fusarium hyphae disappeared, the nematodes covered the plat...

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Abstract

The invention discloses a pseudomonas strain and application thereof. The strain is preserved in China Center for Type Culture Collection on July 24th, 2020, and the preservation number is CCTCC NO: M2020346. The strain can prevent and treat various plant soil-borne diseases, particularly has a remarkable prevention and treatment effect on plant nematode diseases and soybean root rot, and lays a foundation for research and development of biological prevention and treatment of the soil-borne diseases; and the strain can also promote plant growth, is beneficial to promoting agricultural sustainable development, and has a good application prospect.

Description

technical field [0001] The present invention relates to the field of agricultural microorganisms and biological control, in particular to a bacterial strain of the genus Pseudomonas, including a microbial agent of the bacterial strain of the genus Pseudomonas, and a preparation method of the bacterial agent of the genus Pseudomonas. Application in the control of soil-borne diseases and in promoting plant growth. Background technique [0002] Plant soil-borne disease is a common plant disease, mainly refers to fungi, bacteria, actinomycetes, nematodes and other pathogens living in the soil, which are caused by invading crops from plant roots or stems when conditions are suitable. Soil-borne diseases of plants involve a wide variety of crops, resulting in serious production of major crops worldwide and causing economic losses of hundreds of billions of dollars every year. [0003] At present, in agricultural production, the method of preventing plant soil-borne diseases mainl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P21/00A01P3/00A01P5/00C12R1/38
CPCC12N1/20A01N63/27C12R2001/38C12N1/205
Inventor 叶姗丁中
Owner HUNAN AGRICULTURAL UNIV
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