Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Brachymonas sp. LM-R separated from spray paint wastewater bottom mud and application of brachymonas sp. LM-R

A technology of Brevimonas and Monascus, which is applied in the direction of water/sludge/sewage treatment, bacteria, water pollutants, etc., can solve the problems of high operating cost, harsh operating conditions, and low cost, and achieve broad application prospects Effect

Active Publication Date: 2019-07-12
波态(上海)生物科技有限公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When working, use the water curtain to absorb the exhaust gas. After absorbing the exhaust gas, the formed wastewater contains a large amount of benzene series such as benzene and toluene, which must be treated to meet the standard before it can be discharged. Because the benzene series contains a benzene ring structure, it is usually stable in nature. , difficult to degrade, so the existing treatment technology mainly adopts advanced oxidation technology, including: ozone oxidation treatment technology, photocatalytic oxidation treatment technology, electrocatalytic oxidation treatment technology, Fenton oxidation treatment technology, high-voltage pulse plasma treatment technology, etc. The principle of treatment is to use the strong oxidizing properties of substances to directly convert organic matter into CO through oxidation reaction. 2 、H 2 O and other inorganic substances, but they have problems such as harsh operating conditions and high operating costs, which affect their wide application and promotion. Therefore, it is necessary to find cost-effective alternative treatment technologies. Foreign studies have found that microbial technology not only has better treatment effects, but also lowers the cost. It is also low and has no secondary pollution, so microbial technology has received widespread attention, and there are few related technology researches in my country.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Brachymonas sp. LM-R separated from spray paint wastewater bottom mud and application of brachymonas sp. LM-R
  • Brachymonas sp. LM-R separated from spray paint wastewater bottom mud and application of brachymonas sp. LM-R
  • Brachymonas sp. LM-R separated from spray paint wastewater bottom mud and application of brachymonas sp. LM-R

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, screening and identification of degrading bacterial strain

[0025] 1 Materials and methods

[0026] 1.1 Medium and reagents

[0027] Enrichment medium: toluene (5, 10, 20, 30 or 50g, gradually increase the addition for acclimatization and enrichment), CaCl 2 ·6H 2 O0.1g, MgCl 2 0.25g, K 2 HPO 4 1.5g, NH 4 Cl 1g, peptone 9g, H 2 O 500mL, pH=6.5~7.0;

[0028] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O 1000mL, pH4.0;

[0029] Add 1.5% (w / v, 1.5g per 100mL) agar and 50g.L -1 The toluene is formulated into the corresponding solid basal medium;

[0030] pH6.8, toluene concentration 500mg·L -1 , xylene concentration 500mg·L -1 mixed wastewater containing toluene and xylene.

[0031] 1.2 Determination of the effectiveness of bacterial strains on the treatment of mixed wastewater containing toluene and xylene

[0032] The purified single strain was measured...

Embodiment 2

[0067] Embodiment 2, bacterial strain is to containing toluene and xylene mixed wastewater treatment performance detection

[0068] 1 Materials and methods

[0069] 1.1 Medium and reagents

[0070] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH4.0;

[0071] pH7.0, toluene concentration 400mg·L -1 , xylene concentration 500mg·L -1 of mixed wastewater.

[0072] 1.2 The performance of the strain on the treatment of mixed wastewater containing toluene and xylene

[0073] The purified single strain was measured as OD 415nm = 0.2 inoculated into 100mL mixed wastewater (pH7.0, toluene concentration 400mg L -1 , xylene concentration 500mg·L -1 ) in a 250mL Erlenmeyer flask, using mixed wastewater without inoculation as a control, at 30°C, 180r·min -1 Shaking aerobic culture on a constant temperature shaker for 2 days. After the cultivation, the removal rate of the bacterial stra...

Embodiment 3

[0080] Example 3: Detection of bacterial strains on the treatment performance of wastewater containing toluene

[0081] 1 Materials and methods

[0082] 1.1 Medium and reagents

[0083] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH4.0;

[0084] pH7.0, toluene concentration 600mg·L -1 waste water.

[0085] 1.2 The performance of the strain on the treatment of formazan-containing wastewater

[0086] The purified single strain was measured as OD 415nm = 0.2 inoculated into 100mL waste water (pH7.0, toluene concentration 600mg L -1 ) in a 250mL Erlenmeyer flask, with waste water not inoculated as a control, at 30°C, 180r·min -1 Shaking aerobic culture on a constant temperature shaker for 2 days. After the cultivation, the removal rate of the bacterial strain LM-R to toluene was measured according to the method in Example 1.

[0087] 2. Results

[0088] Strain LM-R at pH7.0,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a strain of brachymonas sp. LM-R separated from waste gas leaching wastewater bottom mud in a spray paint room and application of the brachymonas sp. LM-R in the treatment ofwastewater containing methylbenzene or xylene. Specifically, the invention further discloses brachymonas sp. LM-R separated from spray paint wastewater bottom mud, namely the brachymonas sp., and thepreservation number is CGMCC No.17167. The gene sequence of the strain 16S rDNA is shown in SEQ ID NO.1, and the strain 16S rDNA can be used for treating the wastewater containing the methylbenzene orxylene.

Description

technical field [0001] The invention relates to a Brachymonas sp. LM-R strain isolated from waste gas leaching waste water sediment in a spray booth and its application in the treatment of waste water containing toluene or xylene. Background technique [0002] In the process of manufacturing, processing and maintenance of mechanical equipment, etc., baking and spraying operations are often carried out, and a large amount of organic gases with a strong pungent odor will be produced during the baking and painting process. These gases contain a large amount of benzene series such as toluene and xylene. These substances in the spray exhaust gas have strong biological toxicity, irritation and carcinogenicity. Its toxicity and carcinogenicity are mainly benzene series such as toluene and xylene. These waste gases discharged into the environment will cause serious harm to people exposed to these gases for a long time. When practitioners are exposed to organic solvents for a long ti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
CPCC02F3/34C02F2101/322C12N1/205C12R2001/01
Inventor 谢晓梅廖敏丁亚丽廖泽淇梁雨琦
Owner 波态(上海)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com