Mixed bacterium agent capable of degrading toluene, xylene and acrylic acid water mixed waste water and application of mixed bacterium agent
A technology of mixing bacterial agents and mixing wastewater, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., and can solve the problems of lack of versatility and poor biochemistry.
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preparation example Construction
[0019] 1. The preparation method of LM-R bacterial liquid is: in 100mL synthetic medium (C 6 H 12 O 6 (Glucose) 0.2%, K 2 HPO 4 (Dipotassium Hydrogen Phosphate) 0.7%, KH 2 PO 4 (Potassium dihydrogen phosphate) 0.3%, Na 3 C 6 H 5 O 7 (Sodium Citrate) 0.05%, MgSO 4 ·7H 2 O (magnesium sulfate 7 hydrate) 0.01%, (NH 4 ) 2 SO 4 (ammonium sulfate) 0.1%, H 2 0 100mL), add 3-4 LM-R colony spots through the inoculation loop; after that, the inoculated synthetic medium is shaken and cultured at 30 ° C, 180 r / min, to obtain LM-R bacterial liquid for later use.
[0020] When shaking cultured for 1.5 days, the OD of the obtained LM-R bacterial solution 415nm The value is about 2.943, and the colony content is about 3.7×10 8 pcs / mL.
[0021] LM-R is Brachymonas sp. LM-R with deposit number CGMCC No. 17167.
[0022] The preparation method of above-mentioned synthetic medium is: in H 2 Add C to 100mL 6 H 12 O 6 (Glucose) 0.2g, K 2 HPO 4 (Dipotassium hydrogen phosphate) ...
Embodiment 1
[0026] Embodiment 1, the effect of inoculum on single bacterial strain degrading toluene, xylene and acrylic acid in mixed wastewater
[0027] In a conical flask containing 100 mL of basal medium, toluene, xylene and acrylic acid were mixed and added as carbon sources until the content was 1000 mg / L respectively, and used as the medium to be treated;
[0028] The basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl 0.5g, K 2 HPO 4 1.5g, H 2 O 1000 mL, pH 7.0.
[0029] Add bacterial liquid (LM-R bacterial liquid / LM-W bacterial liquid) to the medium to be treated for inoculation until the initial bacterial volume OD 415nm The values were 0.05, 0.1, 0.15, 0.2, and 0.4, respectively, as each experimental group; with no inoculation as the control, shaking cultured at 30°C, 180 r / min for 1 day, extracted, and the degradation effect was determined. Specifically: after the incubation, draw 2 mL of culture solution, add 4 mL, 4...
Embodiment 2
[0036] Embodiment 2, the effect of different inoculum ratios on bacterial mixed degradation of toluene, xylene and acrylic acid in mixed wastewater
[0037] In a conical flask containing 100 mL of basal medium, toluene, xylene and acrylic acid were mixed and added as carbon sources until the content was 1000 mg / L respectively, and used as the medium to be treated;
[0038] The mixed bacterial liquid (mixed bacterial agent) formed by the combination of LM-R bacterial liquid and LM-W bacterial liquid is added to the medium to be treated for inoculation, and the following 7 experimental groups are respectively set:
[0039] Inoculum OD 415nm LM-R:OD 415nm LM-W: 0.1:0.1, 0.1:0.2, 0.2:0.1, 0.2:0.2, 0.2:0.4, 0.4:0.2, 0.4:0.4, at 30℃, 180r·min -1 The cells were cultured with shaking for 1 day, extracted, and the degradation effect was determined. Each treatment was repeated three times.
[0040] OD 415nm LM-R:OD 415nm LM-W is 0.1:0.1, which means that the mixed bacterial solut...
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