Mixed bacterium agent capable of degrading toluene, xylene and acrylic acid water mixed waste water and application of mixed bacterium agent

A technology of mixing bacterial agents and mixing wastewater, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., and can solve the problems of lack of versatility and poor biochemistry.

Active Publication Date: 2019-12-20
波态(上海)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Acrylic acid has strong acidity, and its toxicity to microorganisms is much greater than that of toluene / xylene, and its biochemical properties are very poor. The mixed wastewater of acrylic acid, toluene and xylene is the core component of the production wastewater of paint manufacturers, because it has the biological toxicity of the three at the same time The superimposed effect of the above results in that the bacteria agents that can be used to remove acrylic acid and the bacteria agents that can be used to remove toluene / xylene in the prior art are not universal. At present, no microbial treatment technology has been used to efficiently degrade and remove acrylic acid, acrylic acid, and xylene in mixed wastewater. The research report on toluene and xylene has also become a difficult point in the development of paint production wastewater microbial treatment technology, and is also the core reason why advanced oxidation technology is currently the main reason for paint production wastewater treatment

Method used

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  • Mixed bacterium agent capable of degrading toluene, xylene and acrylic acid water mixed waste water and application of mixed bacterium agent
  • Mixed bacterium agent capable of degrading toluene, xylene and acrylic acid water mixed waste water and application of mixed bacterium agent
  • Mixed bacterium agent capable of degrading toluene, xylene and acrylic acid water mixed waste water and application of mixed bacterium agent

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] 1. The preparation method of LM-R bacterial liquid is: in 100mL synthetic medium (C 6 H 12 O 6 (Glucose) 0.2%, K 2 HPO 4 (Dipotassium Hydrogen Phosphate) 0.7%, KH 2 PO 4 (Potassium dihydrogen phosphate) 0.3%, Na 3 C 6 H 5 O 7 (Sodium Citrate) 0.05%, MgSO 4 ·7H 2 O (magnesium sulfate 7 hydrate) 0.01%, (NH 4 ) 2 SO 4 (ammonium sulfate) 0.1%, H 2 0 100mL), add 3-4 LM-R colony spots through the inoculation loop; after that, the inoculated synthetic medium is shaken and cultured at 30 ° C, 180 r / min, to obtain LM-R bacterial liquid for later use.

[0020] When shaking cultured for 1.5 days, the OD of the obtained LM-R bacterial solution 415nm The value is about 2.943, and the colony content is about 3.7×10 8 pcs / mL.

[0021] LM-R is Brachymonas sp. LM-R with deposit number CGMCC No. 17167.

[0022] The preparation method of above-mentioned synthetic medium is: in H 2 Add C to 100mL 6 H 12 O 6 (Glucose) 0.2g, K 2 HPO 4 (Dipotassium hydrogen phosphate) ...

Embodiment 1

[0026] Embodiment 1, the effect of inoculum on single bacterial strain degrading toluene, xylene and acrylic acid in mixed wastewater

[0027] In a conical flask containing 100 mL of basal medium, toluene, xylene and acrylic acid were mixed and added as carbon sources until the content was 1000 mg / L respectively, and used as the medium to be treated;

[0028] The basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl 0.5g, K 2 HPO 4 1.5g, H 2 O 1000 mL, pH 7.0.

[0029] Add bacterial liquid (LM-R bacterial liquid / LM-W bacterial liquid) to the medium to be treated for inoculation until the initial bacterial volume OD 415nm The values ​​were 0.05, 0.1, 0.15, 0.2, and 0.4, respectively, as each experimental group; with no inoculation as the control, shaking cultured at 30°C, 180 r / min for 1 day, extracted, and the degradation effect was determined. Specifically: after the incubation, draw 2 mL of culture solution, add 4 mL, 4...

Embodiment 2

[0036] Embodiment 2, the effect of different inoculum ratios on bacterial mixed degradation of toluene, xylene and acrylic acid in mixed wastewater

[0037] In a conical flask containing 100 mL of basal medium, toluene, xylene and acrylic acid were mixed and added as carbon sources until the content was 1000 mg / L respectively, and used as the medium to be treated;

[0038] The mixed bacterial liquid (mixed bacterial agent) formed by the combination of LM-R bacterial liquid and LM-W bacterial liquid is added to the medium to be treated for inoculation, and the following 7 experimental groups are respectively set:

[0039] Inoculum OD 415nm LM-R:OD 415nm LM-W: 0.1:0.1, 0.1:0.2, 0.2:0.1, 0.2:0.2, 0.2:0.4, 0.4:0.2, 0.4:0.4, at 30℃, 180r·min -1 The cells were cultured with shaking for 1 day, extracted, and the degradation effect was determined. Each treatment was repeated three times.

[0040] OD 415nm LM-R:OD 415nm LM-W is 0.1:0.1, which means that the mixed bacterial solut...

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Abstract

The invention discloses a mixed bacterium agent capable of degrading toluene, xylene and acrylic acid water mixed waste water. The mixed bacterium agent contains brachymonas sp (Brachymonas sp) LM-R and Beijerinckiacase sp (Beijerinckiacase sp) LM-W. Besides, the invention also provides an application of the mixed bacterium agent to degradation of mixed waste water containing toluene, xylene and acrylic acid at the same time. The mixed waste water containing toluene, xylene and acrylic acid is inoculated with the mixed bacterium agent until the inoculum concentration of the brachymonas sp LM-Ris OD<415nm>0.2-0.4, and the inoculum concentration of the Beijerinckiacase sp LM-W is OD<415nm>0.1-0.2, then under an open system, stirring is performed, degradation is performed under the conditionof pH being 6.0-7.0 and temperature being 25-30 DEG C, and the degradation time is at least 24h.

Description

technical field [0001] The invention relates to a mixed bacterial agent for degrading toluene, xylene and acrylic acid mixed waste water and its application in the treatment of toluene and acrylic acid mixed waste water. Background technique [0002] During the manufacturing, processing and maintenance of machinery and equipment, paint spraying is often carried out. The paint used for spraying is often made of thicker raw paint diluted with toluene and xylene. During the paint spraying operation, the sprayed parts will adhere to about 70% of the sprayed paint. About 30% of the paint enters the air of the spray booth in the form of a mist, producing a large amount of organic gas with a strong pungent odor. These gases contain a large amount of toluene, xylene, acrylic acid and other compounds. These substances in the spray exhaust gas have Strong biological toxicity and irritation, it may cause damage to the lungs, liver, kidneys, blood and other organs, and even cause carcin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32C02F101/34
CPCC02F3/34C02F2101/32C02F2101/34C12N1/20
Inventor 谢晓梅廖敏徐娜张宇豪郭佳雯梁雨琦
Owner 波态(上海)生物科技有限公司
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