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Brevimonas lm-r isolated from the bottom sludge of spray paint wastewater and its application

A brevemonas, monomonas technology, applied in water/sludge/sewage treatment, bacteria, water pollutants, etc., can solve the problems of high operating cost, harsh operating conditions, low cost, etc., and achieve broad application prospects Effect

Active Publication Date: 2020-06-02
波态(上海)生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

When working, use the water curtain to absorb the exhaust gas. After absorbing the exhaust gas, the formed wastewater contains a large amount of benzene series such as benzene and toluene, which must be treated to meet the standard before it can be discharged. Because the benzene series contains a benzene ring structure, it is usually stable in nature. , difficult to degrade, so the existing treatment technology mainly adopts advanced oxidation technology, including: ozone oxidation treatment technology, photocatalytic oxidation treatment technology, electrocatalytic oxidation treatment technology, Fenton oxidation treatment technology, high-voltage pulse plasma treatment technology, etc. The principle of treatment is to use the strong oxidizing properties of substances to directly convert organic matter into CO through oxidation reaction. 2 、H 2 O and other inorganic substances, but they have problems such as harsh operating conditions and high operating costs, which affect their wide application and promotion. Therefore, it is necessary to find cost-effective alternative treatment technologies. Foreign studies have found that microbial technology not only has better treatment effects, but also lowers the cost. It is also low and has no secondary pollution, so microbial technology has received widespread attention, and there are few related technology researches in my country.

Method used

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  • Brevimonas lm-r isolated from the bottom sludge of spray paint wastewater and its application
  • Brevimonas lm-r isolated from the bottom sludge of spray paint wastewater and its application
  • Brevimonas lm-r isolated from the bottom sludge of spray paint wastewater and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, screening and identification of degrading bacterial strain

[0025] 1 Materials and methods

[0026] 1.1 Medium and reagents

[0027] Enrichment medium: toluene (5, 10, 20, 30 or 50g, gradually increase the addition for acclimatization and enrichment), CaCl 2 ·6H 2 O0.1g, MgCl 2 0.25g, K 2 HPO 4 1.5g, NH 4 Cl 1g, peptone 9g, H 2 O 500mL, pH=6.5~7.0;

[0028] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O 1000mL, pH4.0;

[0029] Add 1.5% (w / v, 1.5g per 100mL) agar and 50g.L -1 The toluene is formulated into the corresponding solid basal medium;

[0030] pH6.8, toluene concentration 500mg·L -1 , xylene concentration 500mg·L -1 mixed wastewater containing toluene and xylene.

[0031] 1.2 Determination of the effectiveness of bacterial strains on the treatment of mixed wastewater containing toluene and xylene

[0032] The purified single strain was measured...

Embodiment 2

[0067] Embodiment 2, bacterial strain is to containing toluene and xylene mixed wastewater treatment performance detection

[0068] 1 Materials and methods

[0069] 1.1 Medium and reagents

[0070] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH4.0;

[0071] pH7.0, toluene concentration 400mg·L -1 , xylene concentration 500mg·L -1 of mixed wastewater.

[0072] 1.2 The performance of the strain on the treatment of mixed wastewater containing toluene and xylene

[0073] The purified single strain was measured as OD 415nm = 0.2 inoculated into 100mL mixed wastewater (pH7.0, toluene concentration 400mg L -1 , xylene concentration 500mg·L -1 ) in a 250mL Erlenmeyer flask, using mixed wastewater without inoculation as a control, at 30°C, 180r·min -1 Shaking aerobic culture on a constant temperature shaker for 2 days. After the cultivation, the removal rate of the bacterial stra...

Embodiment 3

[0080] Example 3: Detection of bacterial strains on the treatment performance of wastewater containing toluene

[0081] 1 Materials and methods

[0082] 1.1 Medium and reagents

[0083] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH4.0;

[0084] pH7.0, toluene concentration 600mg·L -1 waste water.

[0085] 1.2 The performance of the strain on the treatment of formazan-containing wastewater

[0086] The purified single strain was measured as OD 415nm = 0.2 inoculated into 100mL waste water (pH7.0, toluene concentration 600mg L -1 ) in a 250mL Erlenmeyer flask, with waste water not inoculated as a control, at 30°C, 180r·min -1 Shaking aerobic culture on a constant temperature shaker for 2 days. After the cultivation, the removal rate of the bacterial strain LM-R to toluene was measured according to the method in Example 1.

[0087] 2. Results

[0088] Strain LM-R at pH7.0,...

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Abstract

The invention relates to a strain of Brachymonas sp. LM-R isolated from waste gas leaching waste water sediment in a spray booth and its application in the treatment of waste water containing toluene or xylene. Specifically, the invention discloses a Brachymonas sp. LM‑R isolated from the bottom sludge of spray paint wastewater, which is Brachymonas sp., preservation number: CGMCC No.17167, and the strain 16S The rDNA gene sequence is shown as SEQ ID NO.1, which can be used to treat waste water containing toluene and / or xylene.

Description

technical field [0001] The invention relates to a Brachymonas sp. LM-R strain isolated from waste gas leaching waste water sediment in a spray booth and its application in the treatment of waste water containing toluene or xylene. Background technique [0002] In the process of manufacturing, processing and maintenance of mechanical equipment, etc., baking and spraying operations are often carried out, and a large amount of organic gases with a strong pungent odor will be produced during the baking and painting process. These gases contain a large amount of benzene series such as toluene and xylene. These substances in the spray exhaust gas have strong biological toxicity, irritation and carcinogenicity. Its toxicity and carcinogenicity are mainly benzene series such as toluene and xylene. These waste gases discharged into the environment will cause serious harm to people exposed to these gases for a long time. When practitioners are exposed to organic solvents for a long ti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
CPCC02F3/34C02F2101/322C12N1/205C12R2001/01
Inventor 谢晓梅廖敏丁亚丽廖泽淇梁雨琦
Owner 波态(上海)生物科技有限公司
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