Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antibacterial peptide, composition containing antibacterial peptide and application

A composition and antimicrobial peptide technology, applied in the direction of medical preparations containing active ingredients, medical preparations with non-active ingredients, antibacterial drugs, etc., can solve the problem of rapid activity reduction, difficulty in preparation, poor activity stability, etc. problem, to achieve the effect of low hemolysis rate and good antibacterial activity

Active Publication Date: 2021-08-27
WENZHOU UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After natural antimicrobial peptides are prepared into liquid preparations, the activity decreases rapidly, and the activity stability at room temperature is not good, so it is difficult to prepare stable liquid preparations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibacterial peptide, composition containing antibacterial peptide and application
  • Antibacterial peptide, composition containing antibacterial peptide and application
  • Antibacterial peptide, composition containing antibacterial peptide and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Bacteriostatic activity test

[0029] Add 100 μL of nutrient broth medium to each well of a 96-well plate, and then add 100 μL of an antimicrobial peptide aqueous solution (that is, only a mixed solution of antimicrobial peptide and water) with a concentration of 2048 μg / mL in the first row of the first column , mix the nutrient broth medium and antimicrobial peptide aqueous solution in the well evenly, draw 100 μL from the first row of the first column and add it to the second row of the first column, after mixing evenly, take 100 μL from the second row of the first column and add it to the next One row, repeat until the eighth row of the first column draws 100μL and discards; then dilute Staphylococcus aureus CMCC(B)26003 to 10 4 to 10 5 CFU / mL, inoculate 96-well plates with 100 μL bacterial solution per well, at this time, the final concentration of antimicrobial peptides in the first row of the first column is 1024 μg / mL, and the final concentration in the eighth r...

Embodiment 2

[0036] In vitro hemolysis rate test

[0037] The blood sample used in the detection of the hemolysis rate of animal erythrocytes by antimicrobial peptides is defibrinated sheep blood.

[0038] The detection method of the hemolytic activity rate of the antibacterial peptide is as follows: the erythrocytes are washed with PBS buffer solution (pH7.2, purchased from Beijing Soleibao Technology Co., Ltd.) and then centrifuged (1500r, 15min) to discard the supernatant, and the volume ratio is 92 The 0.9wt% sodium chloride aqueous solution of 8:8 mixed with the erythrocyte liquid after discarding the supernatant, was mixed with 8% erythrocyte suspension, got 8% erythrocyte suspension of 100 μ L in each well of 96 well plate, then to the first Add the antimicrobial peptide aqueous solution (the solution is prepared by antimicrobial peptide and purified water) to the first to third columns, so that the final concentrations of the antimicrobial peptides in the detection wells of the fir...

Embodiment 3

[0044] Sodium hyaluronate, glycerin, mannitol, glycine, poloxamer 188 and citrate buffer were selected, and an orthogonal experiment with 2 levels and 7 factors (see Table 3) was designed, and a total of 8 samples from sample 1 to sample 8 were obtained. The eye drops of the formula are specifically shown in Table 4, wherein the concentration of antimicrobial peptide in the eye drops is 1 mg / mL.

[0045] Table 3 Orthogonal design table with 2 levels and 7 factors

[0046]

[0047] Table 4 antimicrobial peptide eye drops obtained by orthogonal test

[0048]

[0049]The preparation process of antimicrobial peptide eye drops is as follows:

[0050] (1) Weigh sodium hyaluronate, glycine, glycerol and poloxamer 188 respectively according to Table 2.

[0051] (2) Add sodium hyaluronate, glycine, glycerin, and poloxamer 188 into sterile water for injection, and dissolve until there are no obvious white lumps, and filter the insoluble matter with a 0.22 μm filter membrane to o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an antibacterial peptide, a composition containing the antibacterial peptide and application. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No. 1. The antibacterial peptide has antibacterial activity against microorganisms in the genus Staphylococcus, Streptococcus, Enterococcus, Micrococcus, Moraxella, Corynebacterium, Klebsiella, Enterobacter, Serratia, Proteus, Pseudomonas, Morgan morganella, Haemophilus, Flavomonas, Acinetobacter and Propionibacterium.

Description

technical field [0001] The present invention relates to an antimicrobial peptide, which is used in the treatment of blepharitis, hordeolum, dacryocystitis, conjunctivitis or sterility during operation. Background technique [0002] Since the invention of antibiotics, humans have made great achievements in the control and treatment of microbial infections, but with the continuous use of antibiotics, microbial resistance has become a major problem in microbial infection control, so that some microorganisms can no longer be used. There are antibiotics to control. For example, in clinical drug treatment, vancomycin-resistant staphylococci, enterococci and other Gram-negative bacterial infection diseases have become clinical problems worldwide. [0003] Antimicrobial peptides are a class of small-molecule polypeptides widely present in microorganisms, animals and plants. They have antimicrobial activity and are usually composed of 12 to 50 amino acid residues with a molecular we...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/00A61K9/08A61K38/16A61K47/02A61K47/12A61K47/26A61K47/32A61K47/36A61K47/38A61P27/02A61P31/04A61P31/10
CPCC07K14/001A61K9/08A61K9/0048A61K47/36A61K47/32A61K47/38A61K47/26A61K47/02A61K47/12A61P31/04A61P27/02A61P31/10A61K38/00Y02A50/30
Inventor 杨文菁涂宇瀚周起李世军李斌李小芬
Owner WENZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com