17 results about "Sporobacterium" patented technology

This disclosure relates to compositions and methods using genetically modified bacteria that express superoxide dismutase (SOD) and catalase enzymes to reduce oxidative stress in the skin, and can be used for various skin conditions. In some embodiments of this disclosure, the genetically modified bacteria are Gram-positive bacteria, for example, of the genus Corynebacterium.

Provides *Parabacterium* strains, cultures, inoculants, and their applications. Specifically, provides a *Parabacterium* strain, which may be a strain with accession number GDMCC No: 62664 or 62665, or a strain with at least 96.74% identity to the strain's 16S rRNA and / or at least 87% average nucleotide identity to the strain's genome; the strain or products based on the strain have good preventive or therapeutic effects on tumors or cancers, metabolic diseases, inflammatory or autoimmune diseases, infectious diseases, and central nervous system diseases.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel NADP-dependent malic enzyme variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The NADP-dependent malic enzyme variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting NADP-dependent malic enzyme to change the enzymatic activity of the NADP-dependent malic enzyme, and a recombinant microorganism comprising the NADP-dependent malic enzyme variant is capable of efficiently producing L-glutamic acid.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel malate:quinone oxidoreductase variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The malate:quinone oxidoreductase variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting malate:quinone oxidoreductase to change the enzymatic activity of the malate:quinone oxidoreductase, and a recombinant microorganism comprising the malate:quinone oxidoreductase variant is capable of efficiently producing L-glutamic acid.

This invention, entitled "A Composition Containing Pentadecanoic Acid, Parabacterium Species Outer Membrane Vesicles Containing the Composition, and Their Applications," belongs to the field of probiotics and microbial preparations technology. The technical problem this invention aims to solve is that beneficial live bacteria have weak colonization ability in the gut, are easily affected by the gastrointestinal environment, exhibit unstable individual responses, and struggle to simultaneously address immune regulation and barrier repair. This invention provides a composition containing pentadecanoic acid, Parabacterium Species-derived outer membrane vesicles, and their applications. The outer membrane vesicles are derived from *Parabacterium difficile* and are rich in pentadecanoic acid, palmitic acid, and octadecanoic acid. The composition and outer membrane vesicles prepared by this invention can significantly inhibit macrophage M1 polarization and reduce pro-inflammatory cytokines. TNF-α , IL-6 and IL-1 β It can lower the expression level of [something], reduce intracellular reactive oxygen species levels, improve mitochondrial function, significantly alleviate symptoms of colitis induced by sodium dextran sulfate, regulate the intestinal flora structure, and increase the abundance of beneficial bacteria.

The present application relates to a kind of bacteria C1 and trehalose complex for preventing and treating cucumber fusarium wilt and its application method.The bacteria in the bacterial complex provided in the present application is Cronobacter sp.C1, and the preservation number of China General Microbiological Culture Collection Center is CGMCC No.24172.The strain is isolated from the root of cucumber disease-resistant variety, and has strong affinity with plants.Trehalose can promote the colonization of strain C1 in cucumber rhizosphere and the production of acidic metabolites, inhibit the growth and reproduction of cucumber fusarium wilt in cucumber rhizosphere, thereby effectively preventing the serious occurrence of cucumber fusarium wilt.

The present disclosure relates to a microorganism of the genus Corynebacterium in which the activity of fumarate hydratase is enhanced, and a method for producing L-amino acids using same. The microorganism of the genus Corynebacterium with enhanced fumarate hydratase exhibits increased production capability of L-amino acids such as L-arginine, L-citrulline, L-ornithine, O-acetylhomoserine, L-homoserine, and L-histidine, compared to a parent strain in which the fumarate hydratase is not enhanced, and the microorganism with enhanced fumarate hydratase can be widely used for the production of L-amino acids.

This invention discloses the use of phytic acid in the preparation of drugs or functional foods for regulating intestinal flora imbalance caused by Streptococcus mutans, belonging to the field of biomedicine and microecological regulation technology. This invention is the first to discover that oral phytic acid can effectively inhibit the abnormal colonization of Streptococcus mutans in the intestine, restore intestinal flora diversity, increase the abundance of beneficial bacteria (such as Bifidobacterium and Lactobacillus), reduce the proportion of pro-inflammatory bacteria, and improve intestinal barrier function and inflammatory status. This invention provides a new strategy for regulating intestinal flora imbalance caused by Streptococcus mutans.

The invention relates to a method for obtaining a gramicidin S extract from spore-forming Bacillus belonging to the genus Aneurinibacillus, characterised by comprising a solid-liquid extraction step using a solvent selected from the group comprising lactic acid, acetic acid, a biosurfactant extract obtained from corn steep liquors, or combinations thereof, at a temperature equal to or less than 40 °C. The invention also relates to the use of the gramicidin S extract obtained as an antimicrobial agent in the cosmetic and agrochemical industries, and as an antibiotic in the pharmaceutical industry.

This invention relates to the field of environmental microbiology technology, and discloses a rapid aerobic degradation compound microbial agent for use in ecological public toilets under overload conditions, its preparation method, and its application. The compound microbial agent is composed of component A and component B at a live bacteria ratio of 1:1-3. Component A includes a lipolytic strain of *Pichia pastoris* FJQC-XJ-4 (CICC33293) acclimated under high organic load gradient conditions; component B includes a bacteriocin-producing strain of *Lactobacillus plantarum* bio-61663 (CICC24435). Under simulated overload conditions, the compound microbial agent achieves an organic matter degradation rate of 71.8% and a fecal coliform kill rate of 99.8%. The agent exhibits a high survival rate after 180 days of storage at room temperature and is suitable for emergency treatment of excessive sewage loads in scenic areas, highway service areas, and other locations during holidays.

Vegan protein powder, characterized in that the protein powder contains at least rice protein, pea protein, a prebiotic, an amino acid, a probiotic of the genus Bifidobacterium and a probiotic of the genus Lactobacillus, wherein the vegan protein powder is free of sweeteners.

This invention belongs to the field of biological wastewater treatment, specifically relating to a composite microbial agent, its preparation method, and its application. By mass percentage, it comprises 60%-80% bioactive agent and 20%-40% activity protectant. The bioactive agent includes 66.58%-86.58% of *Haloxylon ammodendron* strains, 7.85%-22.85% of *Hymenobacter* strains, and 5.57%-25.57% of *Thiobacillus* strains. The total viable count of the composite microbial agent is not less than 1 × 10⁻⁶. 9 The compound microbial agent was added to distilled water and cultured under anaerobic shaking conditions to obtain a revived bacterial solution. The supernatant of the revived bacterial solution was mixed with the wastewater to be treated at a volume ratio of 1-10:250, and 100-250 mg / L of a compatible solute was added. The wastewater was then treated under anaerobic conditions. After one week of treatment of low C / N ratio, high-salt phenol wastewater using this compound microbial agent, the denitrification rate remained above 95%, and the phenol degradation rate remained above 80%. This effectively solved the problems of low denitrification efficiency and difficulty in removing phenolic compounds from high-salt phenol wastewater, and the process was simple and low-cost.

This disclosure relates to: a Corynebacterium microorganism comprising a protein variant; a polynucleotide encoding the protein variant; or at least one selected from the group consisting of the protein variant and the polynucleotide; and a method for producing L-amino acids using the microorganism.

The objective is to provide a composition that can suppress diseases other than oral diseases. [Solution] The present invention is a growth inhibitor for Fusobacterium bacteria containing cyclodextran and / or its derivatives. Due to its inhibitory effect on Fusobacterium bacteria, it contributes to the treatment and prevention of ulcerative colitis, liver cirrhosis, obesity, colorectal cancer, esophageal cancer, oral cancer, breast cancer, ovarian cancer, cervical cancer, oral squamous cell carcinoma, colorectal cancer, pancreatic ductal adenocarcinoma, premature birth, and endometriosis ulcers.

The present application relates to the technical field of microorganisms, and particularly relates to a prepartum suicidal ideation marker, which comprises an intestinal flora, and the intestinal flora comprises Bacteroides, Sutterella, Parabacteroides, Lachnospiraceae, Oscillospiraceae, Actinomyces, Gemella, Streptococcus and Oribacterium. By detecting the abundance of the above-mentioned flora markers in the fecal sample of a pregnant woman, the risk of prepartum suicidal ideation of the pregnant woman can be effectively diagnosed, a new perspective is provided for the study of the potential mechanism of suicidal ideation, and the diagnosis method has high specificity, high sensitivity and high accuracy, is simple and safe and non-invasive, has low medical cost, has low misdiagnosis rate, is conducive to timely intervention and inhibition of suicidal ideation of the pregnant woman, improves the physical and mental health of the mother and infant, provides accurate intervention guidance for the clinic, and is suitable for clinical popularization and application.

This invention belongs to the field of biotechnology, specifically relating to an antimicrobial peptide for improving intestinal health and combating diarrhea in animals, and its preparation method. The invention prepares a novel antimicrobial peptide with the amino acid sequence shown in SEQ ID NO:1. Experiments have verified that after one week of feeding in an unfamiliar environment, the antimicrobial peptide can slightly increase the levels of propionic acid and butyric acid in the experimental group of cats. Furthermore, the overall BCFAs levels in the experimental group were lower than those in the control group before and after transportation. Although there were no significant differences in α-diversity and β-diversity between the experimental group fed the antimicrobial peptide and the control group, feeding the antimicrobial peptide significantly increased the levels of Bacteroides, Parabacterium, and Prevotella in the experimental group, and significantly reduced the levels of harmful bacteria Salmonella and Actinomyces. Therefore, the antimicrobial peptide of this invention can be used to prepare products for improving intestinal health and combating diarrhea in animals, such as feed additives or oral liquids.

The present application relates to a strain of promoting growth of Bifidobacterium by metabolism and freeze-drying survival of Lactobacillus reuteri CCFM1528, and belongs to the field of microbial fermentation engineering. The present application introduces Bifidobacterium into the fermentation supernatant of Lactobacillus reuteri for culture, so that the maximum biomass of Bifidobacterium CCFM1167 is increased by 90.05%, and the freeze-drying survival rate is increased from 12.52+0.37% to 17.52+1.69%; the maximum biomass of Bifidobacterium longum CCFM1510 is increased by 42.86%, and the freeze-drying survival rate is increased from 0.62+0.02% to 54.35+1.41%.