The invention discloses a preparation method of olive vinegar. The method comprises the following steps: a1, soaking 5-15 parts of washed rice with water for 40-60 min, draining the rice for 0.5 h, cooking the rice for 40-60 min, and spreading and cooling the rice to 25-35 DEG C to preparing cooked rice with
water content of 30%-35%; a2, inoculating the cooked rice with a
sporobacterium suspensionand adding traditional Chinese medicinal
powder, uniformly stirring the mixture and placing the mixture in a constant-temperature and constant-
humidity incubator with
relative humidity of 95% at thetemperature of 38 DEG C for stacking culture for 22-25 h, spreading and cooling the mixture, performing stacking once again, culturing the mixture for 22-25 h at 32 DEG C, turning and spreading
yeast,and preforming ventilation and
drying for 11-13 h by controlling temperature at 40 DEG C. Various nutritious and functional substances such as rich amino acids, minerals, flavonoids and polyphenols in olive juice and olive leaves are fully used, the made olive vinegar is rich in
nutrition and has great health care function, by reasonable
collocation of the olive juice and the olive leaves, use amount of the juice is reduced, and the olive leaves are fully used.