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Bifidobacterium bifidum capable of reducing colon pathological injury and relieving constipation

A bifidobacterium bifidum and microbial agent technology, applied in the field of microbiology, can solve problems such as laxative dependence, achieve the effect of reducing expression, good activity, increasing content or expression

Active Publication Date: 2021-06-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For those with milder conditions, they are often encouraged to eat more foods rich in dietary fiber, such as fresh vegetables, fruits, beans, etc., but some patients with more serious conditions can use osmotic or stimulant laxatives, such as polyethylene glycol, milk, etc. Fructose or anthraquinone derivatives, etc., but such laxatives often cause dependence, and even side effects such as nausea, abdominal pain, and diarrhea. If the patient is severe enough to have obvious lesions in the intestinal tissue, surgery is required for treatment

Method used

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  • Bifidobacterium bifidum capable of reducing colon pathological injury and relieving constipation
  • Bifidobacterium bifidum capable of reducing colon pathological injury and relieving constipation
  • Bifidobacterium bifidum capable of reducing colon pathological injury and relieving constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Embodiment 1: the acquisition of Bifidobacterium bifidum (Bifidobacterium bifidum) CCFM1165

[0105] 1. Isolation and screening of bifidobacteria strains:

[0106] (1) Use a disposable sterile toilet to collect the feces of a 2-year-old boy in Fengqiu City, Henan Province, and place the feces samples in a liquid medium containing MRS+mass percentage (0.05%-0.1%) cysteine ​​of fructooligosaccharides In anaerobic incubator (N2:CO2:H2=80:10:10), enrich for 12h;

[0107] (2) The feces samples were serially diluted with sterile saline, and then spread on the MRS+ mass percentage (0.05%-0.1%) L- On the solid plate of cysteine ​​hydrochloride, cultivate for 24-48h;

[0108] (3) select a single colony conforming to the basic form of bifidobacteria to carry out plate streaking and purification, and screen and isolate the selected strain of bifidobacteria;

[0109] (4) Cultivate the above-mentioned single bacterial colony in liquid MRS+mass percentage (0.05%-0.1%) cysteine ​​c...

Embodiment 2

[0128] Example 2: Relief of constipation-related symptoms induced by loperamide by Bifidobacterium bifidum CCFM1165

[0129] Specific steps are as follows:

[0130] (1) Preparation of Bifidobacterium bifidum CCFM1165 bacterial suspension

[0131] After taking out Bifidobacterium bifidum CCFM1165 from the refrigerator at -80°C, streak it in the MRS solid medium, culture it at 37°C for 48 hours, pick a single colony in the MRS liquid medium, culture it at 37°C for 24 hours, and prepare the seeds liquid;

[0132]The prepared seed solution was inoculated in a new MRS liquid medium with an inoculum size of 2% (v / v), cultivated at 37°C for 24h, and cultured for one generation again in the same way to prepare the fermentation of Bifidobacterium bifidum CCFM1165 liquid;

[0133] Then, the prepared Bifidobacterium bifidum CCFM1165 fermentation liquid was centrifuged at 6000 r / min and 4° C. for 5 min, and then resuspended with 10% skim milk to obtain a bacterial suspension for animal...

Embodiment 3

[0144] Embodiment 3: Bifidobacterium bifidum (Bifidobacterium bifidum) CCFM1165 can reduce the content of somatostatin (SS) in the serum of constipation mice

[0145] Specific steps are as follows:

[0146] (1) C57BL / 6J mice grouping, modeling and treatment methods are the same as in Example 2.

[0147] (2) After the mice were killed on the 40th day, the collected mouse blood was left to stand for 2 hours, and the serum was obtained after centrifugation at 3000×g for 15 minutes. The somatostatin (SS) detection kit was used to perform experiments according to the instructions, and the standard curve The concentration of SS in serum was calculated.

[0148] Studies have shown that somatostatin, as a neurohormone with a wide range of effects, can inhibit the release of various gastrointestinal hormones, thereby inhibiting gastrointestinal motility.

[0149] Experimental results such as figure 2 As shown, model group (the content of somatostatin SS is 6.41ng / L) has significant...

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Abstract

The invention discloses a bifidobacterium bifidum capable of reducing colon pathological injury and relieving constipation, and belongs to the field of microorganisms. The bifidobacterium bifidum CCFM1165 provided by the invention can effectively improve the first black stool time, the intestinal tract propulsion rate, the stool water content and the pathological damage of colon tissues of a constipation mouse. The strain provided by the invention can influence the content of excitement type and inhibition type gastrointestinal active peptides, and mainly increases the content of two short-chain fatty acids, so that the constipation symptom is relieved. Meanwhile, the bifidobacterium bifidum can effectively increase the abundance of bifidobacteria in excrement and can also relieve pathological damage of colon tissue, so that the effect of comprehensively relieving constipation diseases is achieved. In addition, compared with GDMCC NO.60939, the content of MTL in serum is better increased, secretion of 5-HT in colon tissue is promoted, and the propelling effect on small intestine peristalsis is more remarkable, so the GDMCC NO.60939 can be applied to disease control in a more targeted mode.

Description

technical field [0001] The invention relates to a strain of bifidobacterium bifidum which can reduce colon pathological damage and relieve constipation, and belongs to the field of microorganisms. Background technique [0002] With the development of social economy and the change of people's diet structure, constipation, as a disease that seriously affects the quality of life, has received more and more attention. Epidemiological surveys have shown that the incidence of constipation worldwide is 0.7%-79% (16% on average); only in my country there is an incidence of 3%-17%. [0003] Constipation is mainly manifested as slow transmission of intestinal contents, increased absorption of water in feces, decreased frequency and frequency of defecation, weakened or even disappeared urge to defecate, accompanied by symptoms of abdominal distension and dry stool. To be precise, the judgment of constipation can be based on the Rome IV criteria. For patients with long-term constipati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L29/00A23L2/38A23L19/20A23C9/123A23C13/16A23C19/06A23L11/50A23L11/65A61K35/745A61P1/10C12R1/01
CPCC12N1/20A23L33/135A23L29/065A23L2/382A23L19/20A23C9/1234A23C13/16A23C19/062A23C11/106A61K35/745A61P1/10A23V2002/00A23V2400/517A23V2200/3204A23V2200/30
Inventor 王刚柴茂王琳琳赵建新陈卫张灏
Owner JIANGNAN UNIV
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