Microorganisms for assimilating oils or fats, etc. and methods for treating waste liquids and deodorizing by using the same

A technology of microorganisms and mixtures of microorganisms, applied in the field of microorganisms

Inactive Publication Date: 2006-07-19
KANBIKEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] According to the above-mentioned situation, the inventor has carried out extensive research and exploration to solve various difficulties and problems existing in the prior art as mentioned above, and has developed the technology of applying the above-mentioned new microbial mixture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Soil samples were collected in Oita City, Oita Prefecture, Japan, and sewage was collected from sewage pipes in Oita City, Oita Prefecture, Japan. A certain amount of each specimen was added to a 500-liter container containing a broth medium consisting of a culture mixture, and diluted with hot water extracts (50 g / L) of fish and pork. Thereafter, under aerobic conditions (ventilated by adding air at a rate of 60 liters per cubic meter per minute), the culture medium was continuously cultivated at 15° C. to 20° C. for 2 years, thereby selecting a medium having the set characteristics and Microbes that can assimilate oil or fat, etc., and finally obtain a variety of strains of microorganisms that can effectively decompose waste oil, fat, etc. contained therein. In this example, the incubation was performed under conditions that were practically close to the in situ conditions of the grease trap.

Embodiment 2

[0045] The culture mixture consisting of the new microorganisms selected in Example 1 was diluted and spread on a nutrient agar medium so that the microorganisms were grown on the medium. Single colonies were then isolated from the culture medium and three types of microorganisms were assayed and identified as strains 2629-1b, 2629-2b and 2629-3b based on their microbial properties.

[0046] These strains were collected from the culture medium, and they were mixed in the same amount to obtain a culture mixture, and it was found that the culture mixture of three strains of corresponding microorganisms could effectively decompose waste oil and fat, etc. The bacteriological properties of these strains were studied according to the experimental method described in the Bergey Handbook of Systematic Bacteriology, 8th edition. In Table 1 the bacteriological properties of the strains are listed.

[0047] Experimental content

Strain 2629-1b

Strain 2629-2b

Strain 2...

Embodiment 3

[0051] Example 3: Determination of the partial base sequence (SEQ ID#1) of the 16SrDNA of bacterial strain 2629-1b

[0052] From MicroSeq TM As a result of the analysis, it was found that the partial base sequence of the 16S rDNA of the bacterial strain 2629-1b had the highest homology with Sphingomonas adhaesiva (Int.J.Syst.Bacteriol.199040:320-321) as 97.66%. It also shows that the 16SrDNA of bacterial strain 2629-1b is contained in the cluster formed by the 16SrDNA of Sphingomonas, and it is related to Sphingomonas echinoids (Dinner et al., Int.J.Syst.Bacteriol.1962 49:1103-1109) The 16S rDNA forms a cluster. In addition, the homology search results of GenBank / EMBL / DDBJ by BLAST found that the 16SrDNA of the strain 2629-1b has the highest homology of 98.1% with the 16SrDNA of the strain (uncultured bacteria, No. AF443570). In addition, the 16S rDNA of strain 2629-1b was found to have 99.1% homology with the 16S rDNA of Sphingomonaskoreensis JSS-26 (standard strain) (Lee ...

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PUM

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Abstract

The microorganisms for assimilating oils, fats or an organic waste material, etc. according to the present invention can efficiently decompose waste oils, fats or organic waste materials, etc. contained in a waste liquid and reduce concentrations of such waste oils, fats or organic waste materials, etc. to a level lower than a given standard level. The microorganisms for assimilating waste oils, fats or organic waste materials, etc. to be used for the present invention include strains belonging to genus Sphingomonas, genus Nocardia, genus Microbacterium or genus Bacillus or a mixture of the strains belonging to genera Sphingomonas, Nocardia, Microbacterium and / or Bacillus. The present invention is further directed to a method for treating waste liquids containing waste oils, fats or organic waste materials, etc. by using the microorganisms for assimilating oils, fats or organic waste materials, etc. or to a method for deodorizing offensive odors resulting from such waste liquids.

Description

technical field [0001] The invention relates to a microorganism assimilating oil, fat or organic waste, which can effectively decompose the waste oil, fat or organic waste contained in waste liquid, and has no harm to the environment and human body; A method for microbial treatment of waste liquids discharged from facilities or kitchens of restaurants, dining cars, hotels, food or cooking centers, hospitals, etc.; the present invention also relates to a method for eliminating odors, etc. caused by these waste liquids by using the same microorganisms. More specifically, the present invention relates to a microorganism assimilating oil, fat or organic waste, etc., which can effectively decompose waste oil or fat or organic waste, etc. High-concentration n-hexane extracts in these waste liquids; the present invention also relates to a method for treating the waste liquids discharged therefrom and eliminating the odor caused by these waste liquids. Background technique [0002]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34B01D53/38
CPCC02F2101/32C12R1/01C12P39/00C12R1/365A61L11/00C02F2201/483C02F2303/02A61L9/01C02F3/343C12N1/205C12R2001/01C12R2001/365
Inventor 都法男
Owner KANBIKEN
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