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Microbacterium and application thereof in degradation for organic pollutants

A technology of organic pollutants and microbacteria, applied in the field of microorganisms, can solve problems such as endocrine disorders, threats to the health of fetuses and children, and achieve the effect of no secondary pollution and low cost

Active Publication Date: 2015-07-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although bisphenol A has a wide range of uses, studies have found that it can cause endocrine disorders and threaten the health of fetuses and children

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The microbacteria ( Exiguobacterium profundum ) L20 is isolated and purified from sea mud in the South China Sea.

[0027] The separation and purification method is as follows: the acclimatization medium used is inorganic salt medium, and the composition is (g / L): 6.2g Na 2 HPO 4 , 3.0g KH 2 PO 4 , 0.5g NaCl, 1.0g NH 4 Cl, pH 7.0. First, add 3g of sludge to 100mL inorganic salt medium containing 20mg / L bisphenol A, and after acclimatization at 37°C for 60 days, add 10% by volume inoculum to the inorganic salt medium containing 40mg / L bisphenol A and then acclimate for one week , and so on to gradually increase the concentration of bisphenol A for domestication, the concentration of bisphenol A is 40mg / L, 60mg / L, 80mg / L respectively. After the acclimatization, spread the acclimatization solution on the agar plate (beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, agar powder 20g / L, pH7.0) and culture at 37°C for 48h, and select a single colony on Beef extra...

Embodiment 2

[0035] microbacteria ( Exiguobacterium profundum ) L20 single colony was inoculated in 3mL beef extract peptone medium, cultured for 18h (OD 600 0.45) with an inoculum volume of 20% by volume, it was inserted into the inorganic salt medium with a bisphenol A concentration of 20 mg / L and a pH of 8.0, and reacted for 96 hours at 37 ° C on a shaker at 150 rpm. Determine the degradation rate. The mensuration of degradation rate is the same as embodiment 1, and detection result is that degradation rate is 92%.

Embodiment 3

[0037] microbacteria ( Exiguobacterium profundum ) L20 single colony was inoculated in 3mL beef extract peptone medium, and the culture cultured for 18h was inserted into the inorganic salt medium with a bisphenol A concentration of 60mg / L and a pH of 7.0 at an inoculation volume of 40% by volume. ℃, 100rpm shaker reaction for 108h, the degradation rate was measured by high performance liquid chromatography, and the determination method was the same as in Example 1. The test result showed that the degradation rate was 89%.

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Abstract

The invention relates to a microbacterium and application thereof in degradation for organic pollutants, and belongs to the technical field of microorganisms. The strain is obtained by separating and purifying from the sea mud of the South China Sea, is a variant of microbacterium, and named as Exiguobacterium profundum L20, and through authentication, the strain is a Gram-positive rod-shaped bacterium with a size of 1.1-1.2mum*1.4-3.2mum, which is peritrichous, sporeless, facultative anaerobic, positive with catalase, negative with oxidase, and is used for hydrolyzing gelatine, starch and the like, and the strain is collected in China General Microbiological Culture Collection Centre on March 20, 2014, with the collection number of CGMCC NO. 8944; through experimental verification, the strain has capacity of degrading bisphenol A, and a degradation rate of 90-100%, and can be applied to biodegradation for endocrine disruptor bisphenol A.

Description

technical field [0001] The invention relates to a microbacterium and its application in degrading organic pollutants, belonging to the technical field of microorganisms. Background technique [0002] Bisphenol A is currently known as a phenolic methane compound with environmental hormone effects. Bisphenol A is an important organic chemical raw material, an important derivative of phenol and acetone, and has been widely used in the production of epoxy resin, polycarbonate and other polymer materials and plasticizers, flame retardants, antioxidants, Chemical products such as heat stabilizers. Although bisphenol A has a wide range of uses, studies have found that it can cause endocrine disorders and threaten the health of fetuses and children. Most of the pollutants containing bisphenol A are discharged into natural water bodies, first absorbed into aquatic organisms, and then transferred to the biological groups above the food chain through the bioaccumulation effect of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
CPCA62D3/02A62D2101/28C12N1/205C12R2001/01
Inventor 朱道辰谢长校孙建中李成林张佩佩
Owner JIANGSU UNIV
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