23 results about "Bacillus acidiceler" patented technology

The invention relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No. 1. The antibacterial peptide has excellent broad-spectrum antibacterial activity, for example, the antibacterial peptide has antibacterial activity on bacteria of the genus Staphylococcus (Staphylococcus), the genus Acinetobacter (Acinetobacter) and the genus Bacillus (Bacillus). Under the background that the global bacterial drug resistance problem is increasingly severe and traditional antibiotics gradually lose efficacy on multi-drug-resistant bacteria, a new treatment means is provided for treating bacteria such as methicillin-resistant staphylococcus aureus, carbapenem-resistant acinetobacter baumannii and bacillus subtilis which bring huge threats to public health. And a new possibility is provided for solving the problem that no medicine can be used clinically.

The present application relates to a strain of Inhernia Bacillus and its application in ramie degumming, the strain is preserved in Guangdong Microbial Culture Collection Center on September 15, 2025, and the preservation number is GDMCC NO: 66966. When the strain is applied to ramie degumming, the degumming reaction can be carried out at a higher temperature, the degumming efficiency of ramie is effectively improved, the fiber is not damaged, the quality of the fiber is improved, and the strain has high application value in ramie degumming.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The invention provides an efficient marine ibuprofen degradation method based on an algae-bacterium co-culture system. In the algae-bacterium co-culture system, the bacterium is a bacillus strain Cytobacillus firus, and the algae is a cocoon-type algae acl-Entomonis Sp. The invention also discloses a preparation method of the algae-bacterium co-culture system. The method comprises the following steps: inoculating an algae-bacterium co-culture system into artificial simulated marine sewage containing ibuprofen, and fully mixing to obtain a mixed solution; and then culturing under proper culture conditions. The removal rate of ibuprofen within 10 days reaches 80.6%, and the method has the advantages of being low in energy consumption, environmentally friendly and suitable for marine pharmaceutical pollution remediation, and is a green biological technical scheme which is driven by solar energy and is ecologically sustainable.

The present disclosure generally relates to compositions and methods for improving the fecal score of an animal using a new combination of probiotic Bacillus strains and administering the same to the animal. The present disclosure also relates to compositions and methods for improving gastrointestinal health in an animal using a novel combination of probiotic Bacillus strains. The present disclosure further relates to compositions and methods for increasing sugar release and protein solubility of animal feed using a combination of probiotic Bacillus strains.

The present invention provides methods of using a composition comprising one or more bacterial species of the Bacillus genus, rice bran, L-cysteine and a high molecular weight low osmolality carbohydrate for the treatment and / or prevention of type 2 diabetes, pre-diabetes, and / or a symptom thereof in a subject. Further uses of the composition are also provided herein.

The application discloses collagen tripeptide and a preparation method and application thereof, and belongs to the technical field of bioengineering. The preparation method comprises the following steps: obtaining bacillus velezensis with high collagenase yield through separation and identification, the bacillus velezensis belongs to probiotics, and high-activity collagenase is obtained through fermentation culture and purification; taking shark cartilage or blue fish skin as raw materials, and obtaining collagen tripeptide through the following steps of combined enzymolysis of alkaline protease and collagenase after pretreatment, inactivation, centrifugal separation and freeze-drying. The collagen tripeptide prepared by the application has a molecular weight of less than 500 Dalton, contains active components such as GHK tripeptide, is easy to absorb and has high safety, and can be used for preparing antioxidant products, anti-inflammatory products, memory-enhancing products or immunity-enhancing products. The application solves the problems of unsafe collagenase source and low collagen tripeptide preparation efficiency, and has a good industrial application prospect.

There is provided the combination of Bacillus Calmette-Guérin (BCG) vaccine and β-glucan adjuvant for the treatment of a cancer characterized by the presence of protumoral T3 neutrophils in the tumour microenvironment. The β-glucan is characterized by a μ-1,3 glucose backbone. The BCG and β-glucan combination demonstrated a synergistic effect in remodeling the tumour microenvironment to resist conversion of neutrophils into the T3 phenotype. The cancer can be bladder cancer, melanoma, lung adenocarcinoma, head and neck squamous cell cancer, pancreatic adenocarcinoma, low-grade gliomas, esophageal carcinoma, and cervical squamous cell carcinoma.

The present invention relates to a medium composition for enhancing the pectinase productivity of a Bacillus sp. Strain and a method for producing pectinase using the same.

The invention relates to the technical field of civil engineering materials, in particular to a rapid curing material based on a bio-based material and a preparation method and application of the rapid curing material. The rapid curing material comprises the following raw materials in parts by weight: 70-80 parts of a basic bio-based polymer, 9-15 parts of a curing accelerator, 20-30 parts of a plant source modified polymer and 9-15 parts of a reinforcing agent, the basic bio-based polymer comprises the following raw materials: a bacillus strain and a culture medium; the bacillus strain comprises bacillus amyloliquefaciens; the culture medium comprises a yeast extract and a carbon source. By optimizing a bio-based material preparation process and constructing a multi-path synergistic curing system, a rapid, environment-friendly and high-performance curing effect is achieved, and the technical blank of efficient application of bio-based materials in the field of civil engineering is filled.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

The invention relates to a method for obtaining a gramicidin S extract from spore-forming Bacillus belonging to the genus Aneurinibacillus, characterised by comprising a solid-liquid extraction step using a solvent selected from the group comprising lactic acid, acetic acid, a biosurfactant extract obtained from corn steep liquors, or combinations thereof, at a temperature equal to or less than 40 °C. The invention also relates to the use of the gramicidin S extract obtained as an antimicrobial agent in the cosmetic and agrochemical industries, and as an antibiotic in the pharmaceutical industry.

Described herein are Bacillus cells comprising a nucleotide sequence encoding a Botulinum neurotoxin (BoNT) and methods of producing the BoNT.

The instant disclosure generally relates to nucleic acid (DNA) sequences encoding novel signal peptide sequences, nucleic acid (DNA) sequences encoding novel pro-region sequences, recombinant polynucleotides (e.g., vectors, plasmids, expression cassettes, etc.) encoding precursor proteases, recombinant (modified) Bacillus sp. cells expressing a precursor protease and secreting the mature protease into the fermentation broth when fermented under suitable conditions, and the like.

The invention belongs to the technical field of microbial agents, and particularly relates to a bacillus aryabhattai bacterium TYR2, a microbial agent and application of the bacillus aryabhattai bacterium TYR2 in increasing the content of 2-acetyl-1-pyrroline (2-acetyl-1-pyrroline, called 2-AP for short) in rice. According to the bacillus aryabhattai bacterium TYR2 and the microbial agent containing the bacillus aryabhattai bacterium TYR2, the content of an aroma substance 2-AP in rice grains and leaves can be increased, the content is remarkably increased compared with that of a contrast without applying the bacillus aryabhattai bacterium TYR2, and a technical support is provided for increasing the aroma concentration in rice. The invention further provides a method for increasing the content of 2-acetyl-1-pyrroline in rice. The method is easy and convenient to operate, low in cost and easy to manage.

The application discloses Bacillus velezensis and application thereof in prevention and treatment of stem base rot, the classification name of the Bacillus velezensis is GN-22, has been preserved in China typical culture preservation center CCTCC, and the preservation number is CCTCC NO: M2025566, and the preservation date is March 24, 2025.The Bacillus velezensis GN-22 of the application belongs to rhizosphere growth-promoting bacteria, has significant inhibiting effect on different pathogenic fungi such as fusarium graminearum, pseudofusarium graminearum, fusarium asiaticum, fusarium pseudotrichothecioides and fusarium oxysporum f.sp.cubense, plays an important role in biological control of corn, wheat stem base rot and other diseases; meanwhile, the strain has growth-promoting effect and can promote corn growth.The strain of the application has simple culture condition, is easy to preserve and easy to industrialize, and has good development and application prospect.

The present invention relates to the field of protein nanofibrils originating from Bacillus sp. provided in a composition as additive to a biologically active substance, in particular to a microbial substance such as a pesticide or plant beneficial agent. More specifically, the present invention relates to a composition comprising an agriculturally bioactive substance and Alpha(helical) Endospore appendage (A-ENA) protein nanofibril materials, more precisely wherein said A-ENA protein nanofibrils are recombinantly produced and exogenously added to said bioactive substance of the composition, thereby functioning as coformulant or additive, and preferably increasing the biological activity of said substance. In particular, the composition of the invention comprises B. thuringiensis spores or cells and self-assembled (engineered or functionalized) A-ENA protein nanofibrils as coformulant, providing for an improved efficacy and / or application potential as pesticide or insecticide. The present invention further relates to a method for controlling plant growth and protection of plants or parts thereof from pest damage, in particular by applying said composition of the invention in an effective amount or formulation, wherein the presence of said A-ENA protein nanofibril coformulant or additive results in increased efficacy and / or virulence of the bioactive substance of said composition, in particular for bioactive substances comprising Bacillus sp. strains.

The present invention relates to an aqueous composition for reduction of malodour, particularly secondary malodour, or dank malodour from surfaces. It is especially useful for malodour reduction from soft surfaces like fabric which may be sprayed with the aqueous composition and allowed to remain thereon. Optionally such fabric may thereafter be laundered using any known laundering process to deliver enhanced malodour reduction benefits. This is achieved through use of an aqueous surfactant containing composition that comprises a bacillus bacterial spore along with select alkane diol.

The present invention relates to a composition for cleaning a surface while controlling insects. The composition is useful on hard surfaces in home, such as those on appliances, furniture, floors, walls and toilets, as well as on soft surfaces, such as garments, interior decorations, bed sheets and blankets. The use of the composition on such a surface ensures long-lasting cleaning after surface cleaning and prevents insects from being attracted to such a surface for up to 24 hours. This is accomplished by the use of a cleaning composition comprising spores of bacteria of the genus Bacillus, selected essential oil ingredients, and a surfactant.

The invention discloses a chlorohydrocarbon efficient synergistic degradation combined fungicide, a preparation method and application, and belongs to the technical field of microorganisms, the combined fungicide contains any one strain from rhodococcus or flavobacterium, and any one strain from bacillus, acinetobacter or comamonas. Through limitation of specific bacterium genus combination and artificial proportion optimization, the combined bacterium agent realizes a stable synergistic effect among different bacterium genus, the degradation efficiency is remarkably enhanced, and the 24-hour degradation rate of chlorinated hydrocarbon can be increased to 70% or above and is remarkably higher than that of a single bacterium system. Meanwhile, large-scale production can be achieved through the combined microbial agent, the overall cost is lower than that of a conventional scheme when the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, additional secondary treatment is not needed after repairing is completed, and economic benefits are remarkable. When the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, high-temperature treatment or chemical oxidation agents are not needed, and the combined microbial agent conforms to the development direction of current environmental protection laws and regulations and green restoration technologies.

The invention discloses a hermetia illucens antibacterial peptide Hill198 and genetic recombination bacillus subtilis thereof, and belongs to the technical field of genetic engineering and microorganism application. A bacillus subtilis protease deficient strain WB800N is taken as a starting strain and is transferred into a recombinant expression vector pHT43-Hill198, and recombinant bacillus subtilis for efficiently secreting and expressing the Hill198 protein is obtained by virtue of IPTG (isopropyl-beta-d-thiogalactoside) induction. After the recombinant strain is subjected to induced expression, the fermentation supernatant of the recombinant strain has obvious inhibitory activity on escherichia coli O157: H7 and salmonella typhimurium. The recombinant plasmid still keeps stable inheritance after continuous passage for 30 generations, and insertion of exogenous genes does not affect normal growth characteristics of host bacteria. The recombinant bacterium can be used as a feed additive and is used for preventing and treating intestinal diarrhea diseases of livestock and poultry and aquatic animals caused by gram-negative bacteria such as escherichia coli.

An inducible promoter expression system based on the T7 RNA Polymerase (T7 RNAP), lactose repressor (Lad), and a chimeric T7lac promoter (PT7lac), which can be integrated as a single copy into the B. subtilis genome. In the absence of IPTG, Lad strongly represses T7RNAP and PT7lac, and expression of an exemplary ORF—here superfolder green fluorescent protein (sfGFP) reporter protein—is undetectable by flow cytometry. Addition of IPTG de-represses PT7lac, and simultaneously induces expression of T7RNAP, resulting in very high sfGFP levels.

A surface treatment composition comprising: a) from about 1x102 to about 1x109 CFU / g of the composition of Bacillus spores; b) from about 0.01% to about 5% by weight of the composition of a cationic polyglucan.