58 results about "Bacillus acidiceler" patented technology

The present invention relates to the cloning and application of a novel glutamic acid decarboxylase gene. The glutamic acid decarboxylase obtained from Bacillus is induced and expressed in Escherichia coli and its properties are studied. It is found that the enzyme has a pH of 4.0 It has higher activity at ~pH6.0, the optimum temperature is between 45℃~60℃, Vmax is 150~200 U / mg, and Km is 7~9 mmol / L. The recombinant Escherichia coli containing the enzyme was transformed into whole cells, and 500 g / L L-glutamic acid was added by fed-batch feeding, at 37°C, in disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, pH 7.0 After 12 h of conversion, 347.8 g / L of γ-aminobutyric acid was finally produced, and the molar conversion rate was 99.4%. Compared with the existing glutamic acid decarboxylase, the novel glutamic acid decarboxylase has higher catalytic efficiency and wider pH range, and is more suitable for industrial application.

The invention discloses a preparation method of oxalate decarboxylase. The method comprises the following steps: constructing a recombinant expression plasmid vector comprising Bacillus-containing acid induced promoter, a secretion signal peptide, and a fungus-derived oxalate decarboxylase gene prepared under the control of the secretion signal peptide, transforming bacilli normally growing in a pH value being not more than 4.5 by the vector to prepare oxalate decarboxylase recombinant bacteria; and carrying out culture and acid feeding on the recombinant bacteria in a fermentation medium, inducing the recombinant bacteria, and carrying out simple purification to recover generated extracellular oxalate decarboxylase. The extracellular oxalate decarboxylase prepared through the method is used in diagnosis reagent raw materials, medicinal compositions, healthcare foods or formulated foods with special medical uses. The mutated acid induced promoter is adopted to substitute an original acid induced promoter in the recombinant expression vector, so the transformed bacilli have a high expression level.

The invention relates to the technical field of sewage treatment, in particular to a composite microbial strain for degrading "triphenyl" waste gas and a preservation and activation method thereof. The complex microbial species include Bacillus, Pseudomonas, Streptococcus, Rhodococcus and Brevibacterium. The preservation method comprises the following steps: A1, inoculating the compound microbial strains for degrading "triphenyl" waste gas in a sterile enrichment medium at an inoculum amount of 9-11%, and cultivating by shaking to obtain a bacterial liquid; A2, taking the bacterial liquid , protective agent and active stabilizer, mix well and store at -78-82°C. The composite microbial strain of the present invention can efficiently degrade "triphenyl" waste gas, the preservation method is simple and easy to control, and the cost is low, and the activation of the composite microorganism after preservation can maintain a good "triphenyl" degradation ability, which can be benzene series The practical engineering application of waste gas biological treatment can quickly provide microbial strains with high activity to degrade benzene series.

The invention provides a degrading bactericide for eliminating fluoroglycofen-ethyl residues in wastewater, which belongs to the field of organism high technology. The used bacterial strain is a gram staining reaction negative bacterium FC12 which is identified as bacillus. The main biological property of the gram staining reaction negative bacterium FC12 is G-, the gram staining reaction negative bacterium FC12 is straight rod-shaped, the spore of the gram staining reaction negative bacterium FC12 is elliptical, the peritrichous of the bacillus moves, and the size of the gram staining reaction negative bacterium FC12 is 0.5-2.5*1.2-10 microns; catalase is positive; oxidase is negative; and the gram staining reaction negative bacterium FC12 can be used for hydrolyzing starch and liquefying gelatine. Pesticide residue in water can be reduced by more than 90% by direct application of the degrading bacterium product.

The invention discloses a compound preparation for improving the survival rate of Procambarus clarkii cultured and a use method thereof. The preparation mainly includes component A and component B. Among them, component A is 30-50% of Bacillus and 50-70% of aerobic denitrifying bacteria; component B is 10-15% of quorum effect quencher, 10-15% of synergist, and 20-15% of flocculant 30%, surfactant 10‑15%, oxygenator 20‑30%. In this preparation, component B is used first, and component A is used later after expansion. This preparation can improve the oxidation-reduction potential at the bottom of the aquaculture environment, reduce the reproduction of cyanobacteria and Microcystis aeruginosa in the aquaculture water body, and through the synergistic sequential application of component A and component B, based on the quenching of group sensing effect, microbial ecological occupancy, and microbial matrix The principles of competition and inhibition of microbial metabolites reduce harmful bacteria in the bottom environment and harmful algae in the water body, thereby basically eliminating the production of toxic substances such as algae toxins, hydrogen sulfide, ammonia nitrogen, and nitrite, thereby improving the survival rate of Procambarus clarkii. The preparation has the advantages of fast onset of action and strong effect sustainability.