45 results about "Bacillus acidiceler" patented technology

The invention discloses a strain of bacillus acidophilus CNBG-PGPR-17 and an application of the bacillus acidophilus CNBG-PGPR-17. The invention further discloses a preparation method of the bacillus acidophilus CNBG-PGPR-17. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 28, 2021, the preservation number is CGMCC No.23673, and the strain is classified and named as bacillus acidogenes (Bacillus acidogenes). The strain is high in growth speed, good in stability and easy to amplify and culture; the inorganic phosphorus dissolving capacity is high, and the inorganic phosphorus dissolving amount can reach 277.8 mg / kg 7 days after inoculation; small molecular organic acids such as lactic acid and succinic acid can be secreted, and the pH of the solution is reduced; the contents of soil organic matters, soil total nitrogen, available phosphorus and quick-acting potassium can be increased, and the pH value of the soil is reduced; the growth of blueberry seedlings is promoted, and the blueberry quality is improved.

The invention discloses a bacillus AH83 capable of resisting saline and alkaline and promoting growth and application thereof, and belongs to the technical field of microbiology and biology, the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495, and the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495. The AH83 provided by the invention is a new species of bacillus, can inhibit the growth of pathogenic bacteria of cotton fusarium wilt, and has the growth promoting functions of dissolving potassium, generating indoleacetic acid, cellulase, protease, siderophore and the like; meanwhile, the AH83 can survive in a highly saline-alkaline environment, can promote agronomic characters such as plant height, biomass, leaf area and root development in a corn seedling stage when applied in saline-alkaline soil, can also increase soil nutrients and enzyme activity content, and has a promoting effect on lavender seed germination. The strain is of great significance in improving crop yield and stress resistance and developing novel agricultural microbial agents.

The invention relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No. 1. The antibacterial peptide has excellent broad-spectrum antibacterial activity, for example, the antibacterial peptide has antibacterial activity on bacteria of the genus Staphylococcus (Staphylococcus), the genus Acinetobacter (Acinetobacter) and the genus Bacillus (Bacillus). Under the background that the global bacterial drug resistance problem is increasingly severe and traditional antibiotics gradually lose efficacy on multi-drug-resistant bacteria, a new treatment means is provided for treating bacteria such as methicillin-resistant staphylococcus aureus, carbapenem-resistant acinetobacter baumannii and bacillus subtilis which bring huge threats to public health. And a new possibility is provided for solving the problem that no medicine can be used clinically.

The present application relates to a strain of Inhernia Bacillus and its application in ramie degumming, the strain is preserved in Guangdong Microbial Culture Collection Center on September 15, 2025, and the preservation number is GDMCC NO: 66966. When the strain is applied to ramie degumming, the degumming reaction can be carried out at a higher temperature, the degumming efficiency of ramie is effectively improved, the fiber is not damaged, the quality of the fiber is improved, and the strain has high application value in ramie degumming.

The invention relates to the field of industrial fermentation and protein production. Specifically, the present invention relates to a method for producing a protein of interest in a fermentation medium, the method comprising the steps of: a) inoculating the fermentation medium with a Bacillus host cell comprising a gene encoding the protein of interest under the control of a promoter; b) culturing the Bacillus host cell in the fermentation medium under conditions that facilitate growth of the Bacillus host cell and expression of the protein of interest, c) adding sulfate to the fermentation medium to achieve a sulfate concentration of at least 20 mM in the fermentation medium; and d) precipitating and / or crystallizing the protein of interest during culture. Further contemplated is the use of a high sulfate concentration in the fermentation medium for the production of a protein of interest in a Bacillus host cell and a crystalline protein of interest obtained or obtainable by the method of the invention.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The invention provides an efficient marine ibuprofen degradation method based on an algae-bacterium co-culture system. In the algae-bacterium co-culture system, the bacterium is a bacillus strain Cytobacillus firus, and the algae is a cocoon-type algae acl-Entomonis Sp. The invention also discloses a preparation method of the algae-bacterium co-culture system. The method comprises the following steps: inoculating an algae-bacterium co-culture system into artificial simulated marine sewage containing ibuprofen, and fully mixing to obtain a mixed solution; and then culturing under proper culture conditions. The removal rate of ibuprofen within 10 days reaches 80.6%, and the method has the advantages of being low in energy consumption, environmentally friendly and suitable for marine pharmaceutical pollution remediation, and is a green biological technical scheme which is driven by solar energy and is ecologically sustainable.

The invention belongs to the technical field of microbial preparations, and discloses a bacillus inhibitor and a preparation method thereof, and the bacillus inhibitor comprises bacillus or fermentation liquor thereof and inhibitor chemical components. The preparation method comprises the following steps: carrying out first, second and third step-by-step fermentation on the bacillus strains to obtain high-concentration fermentation liquor, adding the chemical components of the inhibitor, and uniformly mixing to obtain the product. The bacillus inhibitor disclosed by the invention can be used for remarkably reducing the conversion of spores into vegetative bodies, improving the spore rate and prolonging the shelf life of microbial inoculum products.

The present disclosure generally relates to compositions and methods for improving the fecal score of an animal using a new combination of probiotic Bacillus strains and administering the same to the animal. The present disclosure also relates to compositions and methods for improving gastrointestinal health in an animal using a novel combination of probiotic Bacillus strains. The present disclosure further relates to compositions and methods for increasing sugar release and protein solubility of animal feed using a combination of probiotic Bacillus strains.

The present invention provides methods of using a composition comprising one or more bacterial species of the Bacillus genus, rice bran, L-cysteine and a high molecular weight low osmolality carbohydrate for the treatment and / or prevention of type 2 diabetes, pre-diabetes, and / or a symptom thereof in a subject. Further uses of the composition are also provided herein.

Engineered, modified, or mutated Bacillus strains that produce keratinases or serine proteases (KerS) that biologically degrade keratin-containing materials, such as feathers; compositions containing these Bacillus strains or the keratinases they produce, and methods for biologically degrading keratin-containing materials using these strains or keratinases.

The present invention provides an alcohol degrading composition comprising one or more strains of the genus Bacillus, rice bran, L-cysteine, and a high molecular weight, low osmotic pressure carbohydrate. Also provided herein are methods of using the compositions.

The application discloses collagen tripeptide and a preparation method and application thereof, and belongs to the technical field of bioengineering. The preparation method comprises the following steps: obtaining bacillus velezensis with high collagenase yield through separation and identification, the bacillus velezensis belongs to probiotics, and high-activity collagenase is obtained through fermentation culture and purification; taking shark cartilage or blue fish skin as raw materials, and obtaining collagen tripeptide through the following steps of combined enzymolysis of alkaline protease and collagenase after pretreatment, inactivation, centrifugal separation and freeze-drying. The collagen tripeptide prepared by the application has a molecular weight of less than 500 Dalton, contains active components such as GHK tripeptide, is easy to absorb and has high safety, and can be used for preparing antioxidant products, anti-inflammatory products, memory-enhancing products or immunity-enhancing products. The application solves the problems of unsafe collagenase source and low collagen tripeptide preparation efficiency, and has a good industrial application prospect.

There is provided the combination of Bacillus Calmette-Guérin (BCG) vaccine and β-glucan adjuvant for the treatment of a cancer characterized by the presence of protumoral T3 neutrophils in the tumour microenvironment. The β-glucan is characterized by a μ-1,3 glucose backbone. The BCG and β-glucan combination demonstrated a synergistic effect in remodeling the tumour microenvironment to resist conversion of neutrophils into the T3 phenotype. The cancer can be bladder cancer, melanoma, lung adenocarcinoma, head and neck squamous cell cancer, pancreatic adenocarcinoma, low-grade gliomas, esophageal carcinoma, and cervical squamous cell carcinoma.

The present invention relates to a medium composition for enhancing the pectinase productivity of a Bacillus sp. Strain and a method for producing pectinase using the same.

An antibacterial protein against Bacillus with the amino acid sequence as set forth in SEQ ID NO: 1 is disclosed. Also disclosed is a method of preparing an antibacterial protein against Bacillus. The method includes: culturing BL21-pBAD-BAL200 cells, the BL21-pBAD-BAL200 cells including a plasmid that comprising a sequence as set forth in SEQ ID NO: 2; inducing the expression of the antibacterial protein; and purifying the antibacterial protein.

The present invention relates to cosmetic and pharmaceutical compositions containing Bacillus spp. Strains or fermentation broth thereof and methods of producing and using such strains.

The invention relates to the technical field of civil engineering materials, in particular to a rapid curing material based on a bio-based material and a preparation method and application of the rapid curing material. The rapid curing material comprises the following raw materials in parts by weight: 70-80 parts of a basic bio-based polymer, 9-15 parts of a curing accelerator, 20-30 parts of a plant source modified polymer and 9-15 parts of a reinforcing agent, the basic bio-based polymer comprises the following raw materials: a bacillus strain and a culture medium; the bacillus strain comprises bacillus amyloliquefaciens; the culture medium comprises a yeast extract and a carbon source. By optimizing a bio-based material preparation process and constructing a multi-path synergistic curing system, a rapid, environment-friendly and high-performance curing effect is achieved, and the technical blank of efficient application of bio-based materials in the field of civil engineering is filled.

Combination therapeutics for the treatment of cancer include the use of immune effector cells, IL-15 based superagonists and one or more immunotherapeutic agents such as Bacillus Calmette-Guerin (BCG).

Provided is a surface expression vector having pgsA, a gene encoding poly-gamma-glutamate synthetase, and a method of expressing a target protein on the microbial surface using the vector. The vector having foreign genes inserted therein is transformed into a microorganism and allows a foreign protein to be stably expressed on the surface of the microorganism.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

The invention relates to a method for obtaining a gramicidin S extract from spore-forming Bacillus belonging to the genus Aneurinibacillus, characterised by comprising a solid-liquid extraction step using a solvent selected from the group comprising lactic acid, acetic acid, a biosurfactant extract obtained from corn steep liquors, or combinations thereof, at a temperature equal to or less than 40 °C. The invention also relates to the use of the gramicidin S extract obtained as an antimicrobial agent in the cosmetic and agrochemical industries, and as an antibiotic in the pharmaceutical industry.

A freeze-dried bacterial consortium of a strain of Acinetobacter sp., a strain of Serratia sp. and a strain of Bacillus sp.; a method for preparing same; and a use and method applying same, to inhibit the development of diseases caused by plant pathogens and to promote plant growth.

Described herein are Bacillus cells comprising a nucleotide sequence encoding a Botulinum neurotoxin (BoNT) and methods of producing the BoNT.

Exemplary embodiments of the present disclosure are directed towards the carrier-based agricultural biofertilizer composition, includes an isolated Bacillus Cereus strain CGAPGPBBS-048 having the deposit accession number KY495213; an agriculturally acceptable liquid formulation including a stabilizer, that may provide stability and support for at least one of the Bacillus species, thereby enhancing the growth and overall health of the plants.

The instant disclosure generally relates to nucleic acid (DNA) sequences encoding novel signal peptide sequences, nucleic acid (DNA) sequences encoding novel pro-region sequences, recombinant polynucleotides (e.g., vectors, plasmids, expression cassettes, etc.) encoding precursor proteases, recombinant (modified) Bacillus sp. cells expressing a precursor protease and secreting the mature protease into the fermentation broth when fermented under suitable conditions, and the like.

The invention belongs to the technical field of microbial agents, and particularly relates to a bacillus aryabhattai bacterium TYR2, a microbial agent and application of the bacillus aryabhattai bacterium TYR2 in increasing the content of 2-acetyl-1-pyrroline (2-acetyl-1-pyrroline, called 2-AP for short) in rice. According to the bacillus aryabhattai bacterium TYR2 and the microbial agent containing the bacillus aryabhattai bacterium TYR2, the content of an aroma substance 2-AP in rice grains and leaves can be increased, the content is remarkably increased compared with that of a contrast without applying the bacillus aryabhattai bacterium TYR2, and a technical support is provided for increasing the aroma concentration in rice. The invention further provides a method for increasing the content of 2-acetyl-1-pyrroline in rice. The method is easy and convenient to operate, low in cost and easy to manage.

The application discloses Bacillus velezensis and application thereof in prevention and treatment of stem base rot, the classification name of the Bacillus velezensis is GN-22, has been preserved in China typical culture preservation center CCTCC, and the preservation number is CCTCC NO: M2025566, and the preservation date is March 24, 2025.The Bacillus velezensis GN-22 of the application belongs to rhizosphere growth-promoting bacteria, has significant inhibiting effect on different pathogenic fungi such as fusarium graminearum, pseudofusarium graminearum, fusarium asiaticum, fusarium pseudotrichothecioides and fusarium oxysporum f.sp.cubense, plays an important role in biological control of corn, wheat stem base rot and other diseases; meanwhile, the strain has growth-promoting effect and can promote corn growth.The strain of the application has simple culture condition, is easy to preserve and easy to industrialize, and has good development and application prospect.

The present invention relates to the field of protein nanofibrils originating from Bacillus sp. provided in a composition as additive to a biologically active substance, in particular to a microbial substance such as a pesticide or plant beneficial agent. More specifically, the present invention relates to a composition comprising an agriculturally bioactive substance and Alpha(helical) Endospore appendage (A-ENA) protein nanofibril materials, more precisely wherein said A-ENA protein nanofibrils are recombinantly produced and exogenously added to said bioactive substance of the composition, thereby functioning as coformulant or additive, and preferably increasing the biological activity of said substance. In particular, the composition of the invention comprises B. thuringiensis spores or cells and self-assembled (engineered or functionalized) A-ENA protein nanofibrils as coformulant, providing for an improved efficacy and / or application potential as pesticide or insecticide. The present invention further relates to a method for controlling plant growth and protection of plants or parts thereof from pest damage, in particular by applying said composition of the invention in an effective amount or formulation, wherein the presence of said A-ENA protein nanofibril coformulant or additive results in increased efficacy and / or virulence of the bioactive substance of said composition, in particular for bioactive substances comprising Bacillus sp. strains.

The present disclosure relates to methods for producing a composition comprising one or more lipopeptides such as e.g. fengycin, iturin or surfactin, comprising the steps a) initiating a fermentation process by introducing bacteria belonging to Bacillus spp. into a suitable fermentation medium; b) maintaining a low level of dissolved oxygen (DO) in the fermentation medium, and c) performing a recovery step to recover the one or more lipopeptides thereby providing a composition comprising the one or more lipopeptides, wherein the low level of dissolved oxygen in step b) is preferably a dissolved oxygen level in the range of 5-35%. A composition obtained by said methods and its use for controlling, preventing or ameliorating an infection in a plant caused by a fungal or bacterial pathogen is also claimed.