Bacillus subtilis FC12 for degrading fluoroglycofen-ethyl and application thereof

A technology of acifluorfen and Bacillus, which is applied in the biological high field, can solve problems such as residues, achieves low production and use costs, solves the problems of excessive pesticide residues and herbicide phytotoxicity, and improves added value.

Active Publication Date: 2014-12-03
JIANGSU CHANGQING AGROCHEMICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of fluorine-containing pesticides leads to the residue of such pesticides in the soil and water environment, which may pose a potential threat to the ecological environment and human health. Therefore, environmental behaviors such as the migration, transformation and decomposition of fluorine-containing pesticides in the environment are increasingly being focus on

Method used

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  • Bacillus subtilis FC12 for degrading fluoroglycofen-ethyl and application thereof
  • Bacillus subtilis FC12 for degrading fluoroglycofen-ethyl and application thereof
  • Bacillus subtilis FC12 for degrading fluoroglycofen-ethyl and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening and isolation of acifluorfen-degrading Bacillus FC12

[0026] Get 3.0ml of activated sludge obtained from the waste water treatment tank of a certain pesticide factory production workshop that produces acifluorfen, and add it into 100ml inorganic salt liquid culture that the concentration of acifluorfen is 100mg / L, and its formula is: Liter contains 1.50 g K 2 HPO 4 , 0.50 g KH 2 PO 4 , 0.20 g MgSO 4 ﹒ 7H 2 O, 1.00 g NaCl, 1.00 g (NH 4 ) 2 SO 4 , pH 7.0, cultured with shaking at 160r / min, 30°C, transferred to fresh inorganic salt medium every 5 days at 3% inoculum, and transferred 5 times in a row.

[0027] Take 1.0ml of the enriched bacteria solution obtained above, add 9.0ml of sterile water, and make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added to 9.0ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solution...

Embodiment 2

[0029] Example 2 Shake Flask Degradation Experiment of Bacillus FC12

[0030] In the inorganic salt medium containing 100 mg / L acifluorfen, the formula is: 1.50 g K per liter 2 HPO 4 , 0.50g KH 2 PO 4 , 0.20g MgSO 4 ﹒ 7H 2 O, 1.00g NaCl, 1.00g (NH 4 ) 2 SO 4 , pH 7.0, inoculate YS-03 according to 3% inoculum size, culture with shaking at 30°C, and take samples for measurement after 48 hours. Compared with the blank, by image 3 It can be seen that the absorption peak of the treated sample decreased significantly, and fluorfen was degraded by more than 90%.

Embodiment 3

[0031] Embodiment 3 Containing the preparation of bacillus FC12 bacterial agent

[0032] The original species of acifluorfen-degrading Bacillus FC12 screened and isolated in Example 1 were activated on a petri dish, and the degradation performance was measured, and then inoculated on the inclined surface of a test tube for later use. The test tube was inoculated in a 1000ml shake flask containing 200ml LB medium. The formula of LB medium was: yeast extract 5.00g / L, peptone 10.00g / L, NaCl 10.00g / L, pH 7.0, constant temperature shaking culture to logarithmic phase , ready to inoculate the seed pot. The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a ...

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Abstract

The invention provides a degrading bactericide for eliminating fluoroglycofen-ethyl residues in wastewater, which belongs to the field of organism high technology. The used bacterial strain is a gram staining reaction negative bacterium FC12 which is identified as bacillus. The main biological property of the gram staining reaction negative bacterium FC12 is G-, the gram staining reaction negative bacterium FC12 is straight rod-shaped, the spore of the gram staining reaction negative bacterium FC12 is elliptical, the peritrichous of the bacillus moves, and the size of the gram staining reaction negative bacterium FC12 is 0.5-2.5*1.2-10 microns; catalase is positive; oxidase is negative; and the gram staining reaction negative bacterium FC12 can be used for hydrolyzing starch and liquefying gelatine. Pesticide residue in water can be reduced by more than 90% by direct application of the degrading bacterium product.

Description

technical field [0001] The invention belongs to the high-tech field of biology, uses microorganisms to degrade chemical pesticides, is suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production, and specifically relates to an acifluorfen-degrading bacillus FC12 and its application. Background technique [0002] Fluorine-containing pesticides are emerging pesticides that have been used in recent decades. Fluorine atoms have the characteristics of small radius and high electronegativity. Introducing fluorine atoms and fluorine-containing groups (such as: -CF3) into biologically active organic compounds can greatly improve the stability and physiological activity of organic fluorides. So it has become a hot spot of research and development today. However, the extensive use of fluorine-containing pesticides leads to the residues of such pesticides in the soil and water environment, which may pose a potential th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/07A62D101/04A62D101/28
Inventor 于国权周汝祥杜刚吕良忠
Owner JIANGSU CHANGQING AGROCHEMICAL CO LTD
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