Corynebacterium sp strain and application thereof

A technology of Corynebacterium and bacterial strains, applied in Corynebacterium strains and its application fields, can solve the problem of difficult wastewater discharge up to standard, and achieve the effects of protecting human health, low production and use costs, and wide degradation spectrum

Inactive Publication Date: 2012-03-21
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult for general biological treatment to make the effluent of the wastewater containing the above components meet the discharge standards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the acquisition and identification of bacterial strain SG-20

[0026] 1. Obtaining of strain SG-20

[0027] In August 2008, 2 g of activated sludge from coking wastewater was taken from Shaoguan, Guangdong, and added to phenol-containing basal salt medium for 7 days of cultivation. After dilution, it was spread on a phenol-containing basal salt medium plate. After 7 days of cultivation, a single Colonies were preserved after streaking and purification.

[0028] 2. Identification of strain SG-20

[0029] Morphological characteristics: The bacteria are rod-shaped.

[0030] Physiological and biochemical characteristics: the main biological characteristics are G + , Gram-positive bacteria; catalase reaction and glucose fermentation acid production are positive; nitrate reduction, gelatin hydrolysis, nitrite reduction reaction, methyl red reaction, oxidase, V.P. reaction, indole reaction are negative; use Citric acid and propionate, which cannot utilize tar...

Embodiment 2

[0034] Embodiment 2, the degradation rate of bacterial strain SG-20 to different compounds in basic salt liquid medium

[0035] Embodiment 2, bacterial strain are to the degradation rate of different compounds in basal salt liquid medium

[0036] Basal salt liquid medium: mix 1.0g NaCl, 1.0g NH 4 NO 3 , 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 and 0.2g MgSO 4 ·7H 2 O was made up to 1L with water.

[0037] Pick a single colony of SG-20 and put it in 3ml LB liquid medium, shake it at 30°C and 165 rpm for 24 hours to obtain fresh bacterial liquid. Into 100ml of solution A (or solution B or solution C), insert 5ml of fresh bacterial solution, culture on a shaker at 30°C (165 rpm), and take a sample after 3 days (72 hours) to determine the degradation rate of the compound. The results are shown in Table 1.

[0038] The preparation method of solution A: add phenol and chromium (VI) into the basal salt liquid medium, the final concentration of phenol is 100 mg / L, and the final conce...

Embodiment 3

[0044] Embodiment 3, the degradation rate of bacterial strain SG-20 to phenol and the removal rate to chromium (VI) in tannery wastewater

[0045] Pick a single colony of SG-20 and put it in 3ml LB liquid medium, shake it at 30°C and 165 rpm for 24 hours to obtain fresh bacterial liquid. Measure 100ml of tannery wastewater, and detect the concentration (initial concentration) of chromium (VI) therein. The initial concentration of chromium is about 2mg / L. Add phenol to the tannery wastewater to make the final concentration 100mg / L; add 4ml of fresh bacterial solution, mix well, place it on a shaker at 30°C and 165rpm for cultivation, and take a sample after 3 days (72 hours) to determine the degradation rate of the compound . The results are shown in Table 2.

[0046] Table 2 Degradation rate of phenol and removal rate of chromium (VI) in tannery wastewater by strain SG-20 (3 days)

[0047] substrate

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Abstract

The invention discloses a corynebacterium sp strain and application thereof. The bacterial strain provided by the invention is (corynebacterium sp) SG-20 CGMCC NO.3557. The active ingredient of microbial inoculum provided by the invention is (corynebacterium sp) SG-20 CGMCC NO.3557. The bacterial strain has wide degradation spectrum and the degradation rate for a plurality of toxic compound can be more than 90 percent. The microbial inoculum has the advantages of low manufacture and use cost, convenient use, wide degradation spectrum and good elimination effect. The bacterial strain and the microbial inoculum are applicable to the biological processing of phenol, chrome (VI), parachlorophenol and other organics in chromate waste water generated in the tanning and printing processes and the waste water generated in other petrochemical industry process, and can ensure the content of the phenol, the chrome (VI), the parachlorophenol and other organics in the waste water to be in accordance with the waste water emission standard. The invention successfully solves the problem that the effect of eliminating the phenol, the parachlorophenol and other compound in the chromate organic waste water is not ideal, reduces the workload in the manufacture and use processes as well as the use and production cost, and has great significance in protecting environment and the health of people.

Description

technical field [0001] The invention relates to a corynebacterium strain and application thereof. Background technique [0002] Phenol, p-chlorophenol, benzene and their derivatives are important chemical raw materials. Studies have found that these compounds are harmful to humans and environmental organisms. Chromium (VI) is an important component of many chemical wastewaters. Chromium (VI) has obvious toxic effects on the human body. The presence of chromium can significantly inhibit the biological removal of organic pollutants in wastewater. It is difficult for general biological treatment to make the effluent of the wastewater containing the above components meet the discharge standards. Microorganisms are often used as an important tool for environmental pollution remediation because of their powerful degradation functions and strong adaptability. Contents of the invention [0003] The object of the present invention is to provide a corynebacterium strain and applic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/15A62D101/28A62D101/43C02F101/22C02F101/34C02F101/36C02F103/24
Inventor 吴晓磊孙纪全汤岳琴刘伟强赵晶晶郭鹏
Owner PEKING UNIV
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