Bacillus high temperature bacteria NJAU-N30 for accelerating composting and decomposition and application thereof

A bacillus and composting technology, which is applied in the application field of preparing organic fertilizers, can solve the problems of unpredictable H1-7 bacterial agents, accelerate compost maturity, etc., and achieve the effects of promoting temperature rise, promoting germination index, and promoting growth

Active Publication Date: 2019-06-14
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, those of ordinary skill in the art cannot predict whether the H1-7 inoculant can accelerate compost decomposing when used alone

Method used

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  • Bacillus high temperature bacteria NJAU-N30 for accelerating composting and decomposition and application thereof
  • Bacillus high temperature bacteria NJAU-N30 for accelerating composting and decomposition and application thereof
  • Bacillus high temperature bacteria NJAU-N30 for accelerating composting and decomposition and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, isolation and identification of functional strains

[0037] Put the samples in the high temperature phase of composting into a 250ml Erlenmeyer flask with glass beads and 100ml of sterile water, shake at 25°C and 170r / min for 20min to form a soil suspension, and draw 10 -2 、10 -3 、10 -4 、10 -5 Dilute 0.1ml of the soil suspension, spread it on the lignocellulose culture plate, repeat each concentration 3 times, culture at 28°C for 5 days, pick and purify the colonies growing on the plate for more than 5 times, and select the colonies that can still grow Transfer to slant culture and store at 4°C for later use.

[0038]Through qualitative and quantitative screening, a bacterial strain was finally obtained named NJAU-N30. The colony of the bacterial strain NJAU-N30 on the LB plate was small, yellow and shiny, round in shape, slightly raised in the middle, and scattered round in the edge. The bacteria are soft, have a certain viscosity, and are easy to sti...

Embodiment 2

[0047] Determination of various enzyme activities of NJAU-N30 under embodiment 2, high temperature growth conditions

[0048] Use LB medium as NJAU-N30 enzyme production medium: 10g peptone, 5g yeast powder, 10g NaCl, 1000ml deionized water, pH 7.0, sterilized at 121°C for 20min. Use 3ml of sterile water to prepare the bacteria suspension, take 0.5ml and inoculate them in the enzyme-producing medium respectively, and culture on a shaking table at 50°C at 170r / min. After 24 hours, the bacterial liquid was taken, and the extract was added to extract, and the cell membrane was broken to form a stock solution by using an ultrasonic breaker (300W, ultrasonic for 3s, with an interval of 7s, for 3min).

[0049] Cellulase activity was determined according to the method of Albrecht et al. (2008). The stock solution was mixed with acetic acid buffer (50mM, pH 5) at a ratio of 1:5 (w / v), shaken on a horizontal shaker for 1 hour, and then centrifuged at 10000r / min at 4°C for 10 minutes t...

Embodiment 3

[0056] Embodiment 3, the generation of liquid inoculum

[0057] Streak the NJAU-N30 strains stored in glycerol tubes at -80°C on LB solid medium plates for activation, and cultivate them in a 37°C incubator for one hour. Pick a single colony of NJAU-N30 and culture it in a 3ml liquid test tube at 37°C with shaking at 170r / min for 10 hours, and the bacterial solution is used as the seed solution. Transfer the seed solution to the LB liquid shake flask with an inoculum amount of 1% (v / v), cultivate it at 37°C and 170r / min until the mid-logarithmic phase (OD600=1.0), and collect the bacteria by centrifugation at 4°C. Wash with distilled water for 3 times, and resuspend with equal volume of distilled water to obtain liquid inoculum.

[0058] The preparation method of the LB culture medium used is as follows, taking the preparation of 1L medium as an example: protein building 10g, yeast powder 5g, NaCl 10g agar 20g, constant volume to 1000ml, pH natural, sterilized at 121°C for 20...

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Abstract

The invention provides bacillus high temperature bacteria NJAU-N30 for accelerating composting and decomposition and a process for preparing an organic fertilizer by fermentation acceleration throughthe same. A strain has an accession number of CGMCC No. 16736, and the application of the strain is to accelerate composting and fermentation. The process includes the steps of evenly mixing livestockmanure and auxiliary materials according to a certain C / N and then inoculating liquid strains; after forming a stack, adopting a stack type fermentation process, and turning over and fermenting. Compared with a control of no adding of strains, the strain adding effectively promotes the rise in temperature of a stack body at an early stage of composting, the stack body is urged to enter a coolingperiod ahead of time, and the improvement of a compost product germination index and the like is promoted. A prepared organic fertilizer can effectively promote the growth of plants.

Description

technical field [0001] The invention belongs to the field of agricultural microbes, and provides a high-temperature bacterium NJAU-N30 of the genus Bacillus that accelerates compost maturity and its application in the preparation of organic fertilizers. Background technique [0002] The development of large-scale aquaculture plays an important role in improving people's living standards, but the continuous scale of aquaculture has brought about a large amount of concentrated discharge of feces and wastewater, which has caused great pressure on the local environment. At present, composting is one of the most economical and effective treatment methods for a large amount of livestock and poultry manure. It can not only solve the problem of environmental pollution, but also can compost the waste into fertilizer or soil conditioner and apply it to the field to improve the soil and increase the fertilizer efficiency. effect. However, the composting methods and technologies of tra...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C12R1/07
Inventor 沈其荣徐谞李荣何宙阳刘超刘东阳
Owner NANJING AGRICULTURAL UNIVERSITY
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