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Microbiological degradation method for nitrobenzene industrial wastewater

A technology for microbial degradation and industrial wastewater, applied in the field of biological treatment of pollutants, can solve the problems of poor biodegradability of nitrobenzene, low ability to resist the impact load of influent water quality, short survival time, etc.

Inactive Publication Date: 2012-06-27
CHONGQING WENTAI ENERGY CONSERVATION & ENVIRONMENTAL PROTECTION TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from the perspective of the molecular structure of nitrobenzene, the symmetry and stability of the benzene ring structure and the strong electron-withdrawing nitro group make the biodegradability of nitrobenzene poor.
When treating nitrobenzene wastewater with a single bacterial species or a simple mixed bacterial agent, the biological activity is poor, the survival time is short, the impact load capacity of the influent water quality is low, and the treatment is not thorough.

Method used

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  • Microbiological degradation method for nitrobenzene industrial wastewater
  • Microbiological degradation method for nitrobenzene industrial wastewater
  • Microbiological degradation method for nitrobenzene industrial wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment treats nitrobenzene-containing wastewater produced during the production process of a pharmaceutical factory in the southwest region. The concentrations of CODcr and nitrobenzene are 2500-5800mg / L and 65-150mg / L, respectively, the water intake is 200 tons / day, and the main biological process is a batch SBR reactor.

[0025] See figure 1 , Adopt the compound bacterial liquid of Pseudomonas, Maltotrophic oligotrophomonas, Acinetobacter baumannii, Aquaspirillum, Acidobacter avenae, Alcaligenes faecalis and Bacillus for domestication culture, three Level expansion training. After the initial dosage of 10 tons of compound bacterial agent, keep the reactor running stably for 20 days, and the effluent CODcr after treatment <100mg / L, nitrobenzene content <0.05mg / L, the effect is better.

Embodiment 2

[0027] For the composite microbial agent used in Example 1, a small test evaluation was carried out in a microbiological laboratory of a university. The main evaluation route is shown in the attachment figure 2 .

[0028] The laboratory simulated wastewater is a pharmaceutical factory wastewater sample with a nitrobenzene content of 216 mg / L and a CODcr of about 14000. The biological treatment reactor used is a batch SBR reactor with a daily treatment volume of 40L and a residence time of 4h (1h). Facilitate mixing with oxygen, 1h continuous aeration, 1h standing for precipitation, 1h effluent), CODcr after treatment <100mg / L, nitrobenzene concentration <0.05mg / L.

[0029] The biological factor analysis of the mixed solution in the reactor by molecular biology shows that the main dominant bacterial groups in the mixed solution are: Pseudomonas, Stenotrophomonas, Acinetobacter, and Metamorphosis. Bacteria, Acidobacter avenae, Pseudomonas perforatum, Pseudomonas alcaligenes,...

Embodiment 3

[0031] In this example, 1) to 3) verify the simulated wastewater treatment effect of a single strain lacking a composite microbial agent. 4) ~ 7) Verify the simulated wastewater treatment effect of a single strain.

[0032] The laboratory simulated wastewater is a pharmaceutical factory wastewater sample with a nitrobenzene content of 300 mg / L and a CODcr of about 20,000. The biological treatment reactor used is a batch SBR reactor with a daily treatment water volume of 40L and a residence time of 4h (1h Facilitate mixing with oxygen, continuous aeration for 1h, standing for 1h for precipitation, 1h for effluent)

[0033] 1) Pseudomonas deficient complex bacteria

[0034] After treatment, the effluent CODcr=18564.46mg / L, and the concentration of nitrobenzene=48.46mg / L.

[0035] 2) Compound bacterial agent Maltostrobotrophomonas hypotrophic

[0036] After treatment, the effluent CODcr=17668.00mg / L, and the concentration of nitrobenzene=186.34mg / L.

[0037] 3) Compound bacterial agent Ac...

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Abstract

The invention aims to provide a microbiological degradation method for nitrobenzene industrial wastewater. According to experimental selection, composite bacteria liquid of Pseudomonas, Stenotrophomonas maltophilia, Acinetobacter baumannii, Aquaspirillum, Acidovorax avenae, Alcaligenes faecalis and Bacillus is provided. Domestication culture and three-stage amplification culture are carried out on the composite bacteria liquid so as to obtain a high efficiency composite microbial inoculum suitable for industrial production. When used to treat nitrobenzene wastewater, the microbial inoculum provided in the invention has the advantages of strong biological activity, long survival time, high capability in resisting impact load of influent quality and thorough treatment.

Description

technical field [0001] The invention belongs to the technical field of pollutant biological treatment, and in particular relates to a treatment method for using microorganisms to degrade nitrobenzene industrial wastewater. Background technique [0002] Nitrobenzene, with the molecular formula C6H5NO2, is an important chemical raw material widely used in industrial production such as the manufacture of aniline, explosives, lubricants, pesticides and spices. With the rapid development of human industrial production, a large amount of nitrobenzene has entered the natural environment. It is estimated that more than 10,000 tons of nitrobenzene are discharged into the environment around the world every year. Nitrobenzene is a highly toxic substance. After being absorbed by human skin or inhaling its vapor from the respiratory tract, it will cause poisoning or even death. There are extremely strict limits on reasonable discharge in wastewater. In my country, the "Comprehensive ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/12C02F101/38
CPCY02W10/10
Inventor 叶姜瑜黄凌黄平李明川
Owner CHONGQING WENTAI ENERGY CONSERVATION & ENVIRONMENTAL PROTECTION TECH
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