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Method for preparing chenopodium vulvaria volatile oil and application of chenopodium vulvaria volatile oil

A kind of technology of volatile oil and quinoa, which is applied in the preparation of volatile oil of chrysanthemum leaf and quinoa and its application field

Inactive Publication Date: 2012-08-01
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antibacterial, bactericidal and antioxidant activities of this volatile oil have not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0017] 1. the preparation method of the volatile oil of chrysanthemum leaf and quinoa of the present invention is as follows:

[0018] Add 5kg of collected fresh chrysanthemum leaves and quinoa into the reaction kettle, add water until the sample is completely soaked, turn on the oil bath to heat, adjust the temperature of the oil bath to about 130 degrees, add a set of condensing device above the reaction kettle, collect and cool it with a conical flask steam, and then put the collected steam in a separatory funnel and let it stand for stratification until no more oil comes out, then take out the oil layer and collect it, and then dry it with anhydrous sodium sulfate to obtain a light yellow liquid with a fragrant taste and a Sweet and floral, the aroma is intense and persistent. This process takes water steam distillation as an example, and it can also be CO 2 Obtained by supercritical extraction: using CO 2 Obtained by supercritical extraction, the collected fresh chrys...

Embodiment approach 1

[0029] Embodiment 1: The volatile oil of chrysanthemum leaf and quinoa inhibits bacteria

[0030] (1) Strain and culture medium

[0031] The strains were provided by the Food College of Jiangnan University, Wuxi City, Jiangsu Province, and the list is as follows:

[0032] belongs to species name Staphylococcus Staphylococcus aureus Streptococcus Streptococcus pneumoniae, Streptococcus lactis Enterobacter Enterobacter aerogenes Salmonella salmonella Shigella Shigella Pseudomonas Pseudomonas aeruginosa Bacillus Bacillus subtilis, Bacillus cereus Escherichia coli Escherichia coli, Enterococcus faecalis

[0033] Beef extract peptone medium: 5 g beef extract, 10 g peptone, 5 g sodium chloride, 1000 mL water, 18 g agar, pH 7.2-7.4, sterilized at 121 °C for 30 min.

[0034] (2) Experimental process

[0035] 2.1 Preparation of bacterial suspension

[0036] Use an inoculation loop to stir up a certain amount of ...

Embodiment approach 2

[0047] Embodiment 2: the volatile oil of chrysanthemum leaf and quinoa inhibits fungi

[0048] (1) Strain and culture medium

[0049] The strains were provided by the Food College of Jiangnan University, Wuxi City, Jiangsu Province, and the list is as follows:

[0050] belongs to species name Candida Candida albicans Cryptococcus Cryptococcus neoformans penicillin Penicillium solanum Aspergillus Aspergillus niger Mucor Mucor racemosa Trichophyton Trichophyton rubrum Microsporum Microsporum Epidermophyton Epidermophyton flocculus

[0051] The medium is complete medium: 10g yeast extract, 10g peptone, 20g glucose, 20g agar, 1000ml water, natural pH. The culture medium, Oxford cups and Petri dishes were all sterilized.

[0052] (2) Experimental process

[0053] Bacterial suspension, experimental process as described in the first implementation process

[0054] (3) Experimental results

[0055] Table 5 An...

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PUM

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Abstract

The invention relates to application of chenopodium vulvaria volatile oil to bacteriostatic / bactericidal and antioxidative products. The chenopodium vulvaria volatile oil can be obtained by a water steam distillation or CO2 supercritical extraction method, and main ingredients of the chenopodium vulvaria volatile oil are identified by gas chromatograph / mass spectrum. The chenopodium vulvaria volatile oil has an obvious effect of killing or inhibiting bacteria such as staphylococcus, streptococcus, enterobacter, salmonella, shigella, pseudomonas, bacillus, escherichia coli and the like and fungi such as candida, cryptococcus, penicillin, aspergillus, mucor, microsporon, trichophyta, epidermophyton and the like. A diphenyl picryl phenylhydrazine (DPPH) method proves that the chenopodium vulvaria volatile oil has the high antioxidant activity, can be widely applied to industry of food, medicines, cosmetics, health-care products and the like, and is used for preparing the bacteriostatic / bactericidal and antioxidative products.

Description

technical field [0001] The invention relates to the preparation of chrysanthemum leaf and quinoa volatile oil and the application of the volatile oil, especially the antibacterial, bactericidal and antioxidative effects of the volatile oil. Background technique [0002] Vegetable oil has its unique composition and biological activity, and has a wide range of uses in spices, medicine, antibacterial, cosmetics, additives, and ecotourism. Plant essential oils are secondary metabolites in plants. They are composed of simple compounds with relatively small molecular weights. They have a certain aromatic smell and are oily liquid substances that can volatilize at room temperature. The chemical composition of plant essential oils is relatively complex. According to the chemical structure, it can be divided into three categories: aliphatic, aromatic and terpenoid compounds and their oxygen-containing derivatives such as alcohols, aldehydes, ketones, acids, ethers, esters, lactones, ...

Claims

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Application Information

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IPC IPC(8): C11B9/02A61K36/185A61K8/97A61P31/04A61P31/10A61P39/06A23L3/3472
CPCY02A50/30
Inventor 廖志新刘超吴玲逯梦娅马柱坤
Owner SOUTHEAST UNIV
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