Salt-tolerant denitrifying bacteria, strain preparation method thereof and application of bacteria
A technology of nitrifying bacteria agent and denitrifying bacteria, which is applied in the field of environmental microorganisms, can solve the problems of incomplete treatment of high-concentration nitrate waste liquid, secondary pollution and salt tolerance effect, and achieve accelerated domestication and start-up efficiency, efficient and stable treatment methods , stable effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0062] Embodiment 1: the isolation identification of bacterial strain of the present invention:
[0063] (1) Enrichment, separation medium and expansion medium:
[0064] Enrichment medium: potassium nitrate 2g, magnesium sulfate heptahydrate 0.2g, dipotassium hydrogen phosphate 1.76g, potassium sodium tartrate 20g, ferrous sulfate heptahydrate 0.005g, calcium chloride 0.02g, ammonium chloride 0.63g, NaCl 5 %, distilled water 1000mL, pH value 7.5.
[0065] Separation medium: Potassium nitrate 2g, magnesium sulfate heptahydrate 0.2g, dipotassium hydrogen phosphate 0.5g, potassium sodium tartrate 20g, NaCl 5%, distilled water 1000mL, pH 7.0-7.2, solid medium plus agar 1.5% and 1mL bromine Musk thymol blue solution.
[0066] Fermentation medium: peptone 8g, yeast extract 4g, sodium chloride 10g, distilled water 1000mL pH value 7.5.
[0067] (2) Isolation and purification of Halomonas bacterial strain:
[0068] Weigh 5g of the acclimatized denitrification sludge into a 250mL an...
Embodiment 2
[0077] Embodiment 2: the preparation of halomonas bacterial agent
[0078] After the Halomonas was activated and cultivated, the Halomonas seed solution was inserted into the fermentation medium according to the inoculum size of 5%, the fermentation temperature was controlled at 35°C, the dissolved oxygen was 3 -10 4 cfu / L of the bacterial suspension, and fill it to finally obtain the halomonas microbial agent.
[0079] The composition of the fermentation medium is: peptone 10g / L, yeast extract 4g / L, sodium chloride 35g / L, the balance is distilled water, pH value 7.5, sterilized at 121°C for 20min, cooled to room temperature after sterilization.
Embodiment 3
[0080] Embodiment 3: the preparation of halomonas bacterial agent
[0081] The salt-tolerant denitrifying bacteria were inoculated into the seed culture medium, the culture temperature was controlled at 35° C., and the culture was shaken at 35 rpm for 48 hours to obtain a seed fermentation liquid.
[0082] Put the halomonas seed fermentation liquid into the fermentation medium according to 10% inoculum amount, control the fermentation temperature at 35°C, dissolved oxygen <0.2mg / L, tank pressure 0.03-0.10Mpa, and shake at 40rpm for 72h.
[0083] After the Halomonas was cultured by fermentation, centrifuge at 7500 rpm for 10 min at 4°C, discard the supernatant to obtain a bacterial cell precipitate, dilute the bacterial cell precipitate with a fermentation medium, and obtain a bacterial activity of 10 3 cfu / L of the bacterial suspension, and fill it to finally obtain the halomonas microbial agent.
[0084] The seed medium consists of: KNO 3 2g / L, MgSO 4 ·7H 2 O 0.2g / L, K ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com