Application of porous materials for bacterial quorum sensing inhibition/disruption

A quorum sensing, bacteria technology, applied in bacteria, applications, antibacterial drugs, etc., can solve problems such as destroying bacterial QS signal molecules

Inactive Publication Date: 2018-12-21
OIL DRI OF AMERICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the strategies reported in the literature achieves the destruction of bacterial QS signaling molecules by external adsorption and / or inactivation of QS signaling molecules using adsorbents / catalytic inhibitors of QS signaling molecules.

Method used

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  • Application of porous materials for bacterial quorum sensing inhibition/disruption
  • Application of porous materials for bacterial quorum sensing inhibition/disruption
  • Application of porous materials for bacterial quorum sensing inhibition/disruption

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0151] Example 1 Adsorption / catalysis of N-butyryl-DL-homoserine lactone on various adsorbents / catalytic inhibitors.

[0152] An aqueous solution of N-butyryl-DL-homoserine lactone was placed in a vial containing a fixed amount of clay to achieve an adsorbent / catalytic inhibitor / analyte ratio of 15 (mg / mg). The suspension was agitated for 30 minutes at 25°C, followed by centrifugation at 4500 rpm for 30 minutes. The supernatant was removed and analyzed directly with HPLC-DAD using the conditions listed in Table 1.

[0153] Table 1. HPLC-DAD method for quantification of N-butyryl-DL-homoserine lactone.

[0154]

[0155] The performance of different adsorbents / catalytic inhibitors for N-butyryl-DL homoserine lactone is provided in Table 2.

[0156] Table 2. Performance of different sorbents / catalytic inhibitors for the removal of N-butyryl-DL homoserine lactone at a sorbent / catalytic inhibitor / QS analyte ratio of 15 (mg / mg).

[0157] Material

Embodiment 2

[0158] Example 2 N-(3-oxooctanoyl)-L-homoserine lactone adsorption / catalytic.

[0159] A 200 ppm aqueous solution of N-(3-oxoctanoyl)-DL-homoserine lactone was placed in a vial containing a fixed amount of clay to obtain a sorbent / catalytic inhibitor / QS analysis of 375 (mg / mg) material ratio. The suspension was agitated at 100 rpm for 15 minutes and centrifuged sequentially at 3,500 rpm for 30 minutes. The supernatant was removed and analyzed directly with HPLC-DAD using the conditions listed in Table 3. Degradation and aggregation products were identified by LC / MS.

[0160] Table 3. HPLC-DAD method for the quantification of N-(3-oxoctanoyl)-DL-homoserine lactone.

[0161]

[0162] Table 4 provides the performance of different adsorbents / catalytic inhibitors for the removal of N-(3-oxoctanoyl)-DL-homoserine lactone.

[0163] Table 4. Different sorbents / catalyst inhibitors for the removal of N-(3-oxooctanoyl)-DL-homoserine lactone at a sorbent / catalyst inhibitor / QS ...

Embodiment 3

[0169] Example 3 Adsorption / catalysis of different adsorbents / catalytic inhibitors by 2-heptyl-3-hydroxyl-4-quinolone (PQS) change.

[0170] A 50% methanol solution containing 100 ppm 2-heptyl-3-hydroxy-4-quinolone was placed in a vial with a fixed amount of clay to obtain an inhibitor / analyte ratio of 100 (mg / mg). The suspension was agitated at 100 rpm for 15 minutes and centrifuged sequentially at 3,500 rpm for 30 minutes. The supernatant was removed and analyzed directly with HPLC-DAD using the conditions listed in Table 3.

[0171] Table 6. HPLC-DAD method for quantification of 2-heptyl-3-hydroxy-4-quinolone.

[0172]

[0173] The performance of different adsorbents / catalytic inhibitors for 2-heptyl-3-hydroxy-4-quinolone is provided in Table 7.

[0174] Table 7. Performance of different clays / modified materials for Pseudomonas quinolone signal (PQS) removal at an inhibitor / QS analyte ratio of 100.

[0175] Material

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Abstract

This application relates to the modulation of the flora of bacteria in an environment by inhibiting the quorum sensing of a specific bacteria in said environment by administering an effective amount of a quorum sensing control composition comprising at least one quorum sensing control agent, which is an adsorbent / catalytic inhibitor for a QS signal molecule, such as, N-acyl homoserine lactones(AHL), pseudomonas quinolone signal (PQS), and autoinducer-1 (AI-1), autoinducer-2 (AI-2) type of quorum sensing molecules. Quorum sensing control agents include a sorbent material, sorbent mineral or non-porous mineral such as, for example, phyllosilicate clays, silica, calcite, zeolites, diatomaceous earth, smectite, activated carbon, a nanoparticle or a combination of any of the foregoing. Methodsinclude inhibiting the spoilage of food stuffs and preventing vibriosis in fish or shell fish.

Description

[0001] Related applications and incorporated by reference [0002] This application claims the benefit of priority to U.S. Provisional Application Serial No. 62 / 302,647, filed March 2, 2016, and U.S. Provisional Application No. 62 / 351,378, filed June 17, 2016. [0003] The foregoing application, and all documents cited therein or referred to during its examination (“Application Citations”) and all documents cited or referred to in Application Citations, and all documents cited or referred to herein (“Herein-Referenced Documents” ), and all documents cited or referred to in documents cited herein, together with any manufacturer's instructions, descriptions, product sheets, and product cards for any product mentioned in any document mentioned herein or incorporated by reference herein, are hereby Incorporated herein by reference and may be used in the practice of the present invention. More specifically, all documents mentioned are incorporated by reference to the same extent as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/78G01N33/00G01N33/02C08K3/34C08K3/36A01N63/50
CPCA01N25/00A01N59/20A61K33/12A61K38/47C12Y302/01017A61P17/00A61P31/02A61P31/04A01N63/50A01N25/12A01N59/06A01N61/00A01N63/00A23L3/358C12N1/20C12Q1/68
Inventor S.P.奈克S.程J.肖林M.A.赫普菲尔F.池
Owner OIL DRI OF AMERICA
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