Culture method for improving high-temperature tolerance of bait algae isochrysis zhanjiangensis

A technology of isochrysis and high temperature tolerance, which is applied in the field of bait microalgae cultivation, can solve the problems of poor repeatability and unstable strain transmission, and achieve the effect of accumulation improvement

Pending Publication Date: 2021-10-22
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, researchers screen Chrysophylla strains with high temperature resistance through molecular genetics, physical mutagenesis, etc., but the screened strains have shortcomings such as unstable passage and poor repeatability.

Method used

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  • Culture method for improving high-temperature tolerance of bait algae isochrysis zhanjiangensis
  • Culture method for improving high-temperature tolerance of bait algae isochrysis zhanjiangensis
  • Culture method for improving high-temperature tolerance of bait algae isochrysis zhanjiangensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Comparison of Isochrysis Zhanjiang IZ at different temperatures

[0024] like figure 1 , Zhanjiang Isochrysis wild strain IZ, prepared the mother solution according to the general f / 2 medium formula, and prepared the medium according to the ratio of f / 2:natural seawater=1:1000. According to the initial density 5×10 4 cells mL -1 , the stock solution was inoculated into a 1L Erlenmeyer flask with a total volume of 600mL, cultured at different temperatures (20°C, 25°C, 30°C and 35°C), with a light intensity of 2500±200lux and a light cycle of 12h:12h Microalgae, turn the bottle and shake by hand three times a day.

[0025] Generally speaking, the suitable temperature for the growth of Isochrysis zhanjiang is 20-25°C, too high (higher than 25°C) temperature will limit the growth of algal cells. Depend on figure 2 It can be seen that under the temperature condition of 20-25°C, the wild strain IZ of Isochrysis zhanjiang had no significant difference 5 day...

Embodiment 2

[0028] Example 2: Effects of different concentrations of IAA on the growth and biomass accumulation of Isochrysis zhanjiang wild-type IZ at high temperature

[0029] Isochrysis zhanjiang IZ was cultured at 35°C, and the other conditions were the same as in Example 1. It was cultured in a constant temperature incubator until the 6th day, and the added concentrations were 0.001, 0.01, and 0.1 mg L -1 The IAA. When preparing the plant growth regulator, the phytohormone was first dissolved in 50% ethanol, and a 10 mM stock solution was prepared with deionized water, and then the phytohormone stock solution was added to the medium at different concentrations.

[0030] like Figure 4 It can be seen that its biomass is at a low concentration of IAA (0.001mg·mL -1 ) was significantly improved under the control, and the highest cell density reached 1.98×10 6 cells mL -1 , the growth rate was also significantly increased on the 7th day;

[0031] like Figure 5 It can be seen that ...

Embodiment 3

[0033] Example 3: Effects of different concentrations of IBU on the growth and biomass accumulation of Isochrysis zhanjiang wild-type IZ at high temperature

[0034] Isochrysis zhanjiang IZ was cultured at 35°C, and other conditions were the same as in Example 1. It was cultured in a constant temperature incubator until the 6th day, and the concentration of IBU was 0.5, 1, and 5 mg L respectively. -1 ,

[0035] like Figure 6 It can be seen that adding a low concentration of IBU (0.5mg·mL -1 ), the biomass of Isochrysis Zhanjiang was always higher than that of the control group, and the highest density reached 1.98×106cells·mL -1 .

[0036] like Figure 7 It can be seen that the content of polysaccharides decreases with the increase of the concentration of ibuprofen IBU, and the polysaccharides produced in the control group and the treatment groups with different concentrations are 17.22%, 33.25%, 23.98% and 14.83%, respectively. After low concentration (0.5mg·mL -1 ) ib...

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Abstract

The invention provides a culture method capable of improving the high-temperature tolerance of isochrysis zhanjiangensis. According to the culture method, an indoleacetic acid and/or jasmonic acid inhibitor is added into a culture medium for culturing the isochrysis zhanjiangensis. On the premise of simple operation and low cost, the high-temperature adaptability of the wild type IZ is adjusted by using a plant growth regulator according to the change characteristics of endogenous plant hormones of the wild type IZ, so that the biomass of the wild type IZ and the accumulation of other secondary metabolites are improved, therefore, the application of the isochrysis zhanjiangensis in the industries of aquatic bait, food, medicine and health care and the like is facilitated.

Description

technical field [0001] The invention belongs to the technical field of cultivating bait microalgae, and in particular relates to a cultivating method for improving the high temperature tolerance of Zhanjiang Isoflagellates. Background technique [0002] Isochrysis zhanjiangensis (IZ for short) is a high-value marine economic microalgae. It has the characteristics of small cells, fast reproduction, rich algae nutrition, and easy absorption by larvae. It is a good basic bait for marine economic organisms. At the same time, the cells of Isoflagellates Zhanjiang are rich in polyunsaturated fatty acids, DHA, EPA, protein, polysaccharides and other highly nutritious substances, which can not only be used as aquatic bait, but also have good application prospects in food, medicine and health care industries. [0003] However, under the general trend of global warming, the growth of wild-type I. dinoflagellates and the accumulation of high-value metabolites have been affected by the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38
Inventor 程鹏飞周成旭严小军汪纯常婷徐继林
Owner NINGBO UNIV
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