Method for simultaneously extracting bait additive rich in DMPT and bait additive rich in fucoxanthin from chrysophyceae

A technology of fucoxanthin and additives, applied in the direction of additional food elements, animal feed, applications, etc., can solve the problems of no reports, differences in food attraction, and growth-promoting effects

Active Publication Date: 2021-05-11
山东省海洋资源与环境研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004]However, there are few research reports on DMPT extracted from seaweed. Currently, DMPT used in aquatic feed is chemically synthesized, and some even have similar chemical structures other compounds; this leads to differences in predative, growth-promoting effects, and even safety concerns
Simultaneously, extract the bait additive that is rich in DMPT and the bait additive that are rich in fucoxanthin from seaweed simultaneously and do not have report especially

Method used

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  • Method for simultaneously extracting bait additive rich in DMPT and bait additive rich in fucoxanthin from chrysophyceae
  • Method for simultaneously extracting bait additive rich in DMPT and bait additive rich in fucoxanthin from chrysophyceae
  • Method for simultaneously extracting bait additive rich in DMPT and bait additive rich in fucoxanthin from chrysophyceae

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Centrifuge the golden algae culture solution in the logarithmic growth phase at a speed of 6000r / min for 10 minutes, collect the algae mud and freeze-dry it at -18°C for 24 hours, and then dry it in a freeze dryer to obtain algal powder;

[0059] Mix 1 g of algae powder and 100 mL of ethanol water solution with a volume concentration of 70%, ultrasonicate at 20°C for 45 min, centrifuge at 4000 rpm for 15 min, and collect the supernatant as the extract;

[0060] In the extract of 100mL, add the deionized water of 100mL, pass through D101 macroporous resin column with the flow rate of 5mL / min, the internal diameter of described D101 macroporous resin column is 1.6cm, and column bed height is 20cm, and column volume is 40mL, get effluent;

[0061] The effluent was rotary evaporated at 60°C to obtain the bait additive rich in DMPT;

[0062] Adopt 60mL volume concentration of 70% ethanol aqueous solution to elute adsorption D101 macroporous resin column, discard the eluent;...

Embodiment 2

[0089] The only difference from Example 1 is that the extractant is an aqueous ethanol solution with a volume concentration of 60%.

Embodiment 3

[0091] The only difference from Example 1 is that the extractant is an aqueous ethanol solution with a volume concentration of 75%.

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Abstract

The invention provides a method for simultaneously extracting a bait additive rich in DMPT and a bait additive rich in fucoxanthin from chrysophyceae, and belongs to the technical field of substance extraction. According to the method, an ethanol aqueous solution with the volume concentration of 60-75% is adopted as an extracting agent, DMPT and fucoxanthin in algae powder can be greatly extracted, the obtained extracting solution passes through a D101 macroporous resin column, fucoxanthin and the like can be adsorbed on the resin column, DMPT flows out of the adsorption column along with effluent, and the obtained effluent is subjected to rotary evaporation to obtain the bait additive rich in DMPT. And by selecting a proper eluent to elute the fucoxanthin adsorbed on the resin column, and carrying out rotary evaporation on the eluent, the bait additive rich in fucoxanthin is obtained. The obtained bait additive is high in DMPT content, the quality of bait is improved, meanwhile, the bait additive rich in fucoxanthin is obtained, and the utilization rate of the raw material chrysophyta is increased.

Description

technical field [0001] The invention relates to the technical field of substance extraction, in particular to a method for simultaneously extracting a bait additive rich in DMPT and a bait additive rich in fucoxanthin from golden algae. Background technique [0002] Chrysophyta belongs to Chrysophyta, Purinphyceae, Isodinophyceae, Isodinophyceae, rich in a variety of nutrients, such as polysaccharides, carotene, fucoxanthin and high-energy lipids, unsaturated fatty acids etc., because it has no cell wall, small individual, fast production and reproduction speed, and easy cultivation, it is an important economic bait algae for mariculture at home and abroad. [0003] Dimethyl-β-thiatin propionate (DMPT) exists in many phytoplankton, macroalgae and molluscs symbiotic with algae, and is a representative substance that algae and plankton convert inorganic sulfur into organic molecules. Studies have reported that DMPT has a healing effect on gastric damage in experimental mice a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32C07C381/12A23K50/80A23K20/121A23K20/105
CPCC07D303/32C07D301/32C07C381/12A23K50/80A23K20/121A23K20/105
Inventor 韩典峰徐英江乔洪金宫向红张华威田秀慧任传博任利华刘慧慧黄会刘小静
Owner 山东省海洋资源与环境研究院
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