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Phaeodactylum tricornutum ZEP1 gene, protein and application

A technology of Phaeodactylum and genes, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of not synthesizing fucoxanthin, etc., and achieve the effect of improving light harvesting efficiency

Active Publication Date: 2022-04-26
WESTLAKE UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report on the gene for the synthesis of fucoxanthin

Method used

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  • Phaeodactylum tricornutum ZEP1 gene, protein and application
  • Phaeodactylum tricornutum ZEP1 gene, protein and application
  • Phaeodactylum tricornutum ZEP1 gene, protein and application

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Experimental program
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Effect test

Embodiment

[0033] 1. Acquisition of ZEP1 gene

[0034] A Phaeodactylum tricornutum ZEP1 gene was screened by various techniques such as transcriptome and metabolome. The nucleotide sequence length of the full-length coding frame of the gene is 1698bp, consisting of 565 amino acids. The nucleotide sequence is shown in the sequence SEQ ID NO .1, its protein sequence is shown in SEQ ID NO.2.

[0035] 2. Phenotypic analysis of crtiso5 mutants

[0036] Previously, there was no report on the function of the ZEP1 gene in Phaeodactylum tricornutum. The applicant conducted the following research and discovered for the first time that the ZEP1 gene plays an important role in the synthesis of fucoxanthin in Phaeodactylum tricornutum.

[0037] 1. Construction of ZEP1 gene editing vector

[0038] Use the CRISPOR website (http: / / crispor.tefor.net / ) to select the guide sequence (sgRNA) for CRTISO5 gene editing, design two primers (Table 1) according to the selected sgRNA, and obtain double strands wi...

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PUM

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Abstract

The invention discloses a phaeodactylum tricornutum ZEP1 gene, a protein and application, and discloses a new function of the ZEP1 gene for the first time, and the ZEP1 gene can improve the fucoxanthin content in phaeodactylum tricornutum; moreover, the fucoxanthin content in photosynthetic organisms (plants, algae and photosynthetic bacteria) can be improved, so that the light harvesting efficiency is improved, the photosynthetic efficiency and biomass accumulation are facilitated, and the protein encoded by the gene can be used for synthesizing a fucoxanthin precursor in vitro.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the Phaeodactylum tricornutum ZEP1 gene, protein and application. Background technique [0002] Carotenoids widely exist in photosynthetic organisms and have dual properties of anti-oxidation and capturing light energy. Due to the diversity of structures, different carotenoids have different application values. A variety of carotenoids, such as fucoxanthin and astaxanthin, have high market value. In recent years, in addition to anti-oxidation, fucoxanthin has also been reported to have anti-cancer functions. [0003] In addition, fucoxanthin has important applications in photosynthetic light energy capture. Blue-green light has a strong penetrating ability in seawater, so most marine algae contain fucoxanthin, which is used to capture visible light in the blue-green band. If fucoxanthin is transferred to other photosynthetic organisms, it can be used to broaden the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/70C12N1/21C12P17/02C12R1/19
CPCC12N9/0077C12N15/70C12P17/02
Inventor 李小波曹天骏
Owner WESTLAKE UNIV
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