Isochrysis galbana propagation method

A technique for the expansion of dinoflagellates and dinoflagellates, which is applied in the field of dinoflagellates propagation, can solve the problems of not considering the influence of algae bacteria, so that it is not easy to decay, and it is beneficial to the control of microalgae quantity and production management Effect

Active Publication Date: 2020-08-04
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the actual microalgae propagation technology does not consider the influence of algae bacteria.

Method used

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  • Isochrysis galbana propagation method
  • Isochrysis galbana propagation method
  • Isochrysis galbana propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening Beneficial Bacteria

[0023] The aseptically purified Isochrysis globosa and the phycobacteria isolated from the algae liquid of Isochrysis globus were respectively cultured to the exponential growth phase. Isochrysis globosa liquid was divided into 100mL sterile Erlenmeyer flasks by 50mL each. Each expanded bacteria solution was collected by centrifugation at 13000 rpm at low temperature for 10 minutes and washed three times with Ningda No. 3 seawater medium to remove the 2216E liquid medium. According to the ratio of algal cell density ratio of 1:50, different algae bacteria were added to the culture medium of Isochrysis globosa, and three parallels were set up in each group, and Ningda No. 3 seawater culture medium was added as the control group. Using the cell density of Isochrysis globosa as an index, the effects of various interphyseal bacteria on the growth of Isoflagellates were analyzed. A total of 5 strains of phycobacteria were isolated...

Embodiment 2

[0026] Embodiment 2: multiplication method

[0027] First prepare a new type of algae expansion culture solution, its composition and proportion are as follows: 50-200mg / L KNO 3 , 5-20mg / LKH 2 PO1-5 mg / L Fe-citrate 5H 2 O, 0.01-0.1g / L brown sugar, 2-10μg / L VB 1 , 0.01-0.1μg / L VB 12 .

[0028] The concrete composition of a kind of concrete algae culture expansion liquid is as follows:

[0029] 100mg / L KNO 3 , 10mg / L KH 2 PO 4 , 3mg / L Fe-citrate 5H 2 O, 0.06g / L brown sugar, 6μg / LVB 1 , 0.05μg / L VB 12 .

[0030] Brown sugar can be selected from the varieties commonly used in the market, and a specific composition is as follows:

[0031] Every 100g brown sugar contains 96.6g sucrose, 0.8μg carotene, 1.9μg retinol, 0.01mg thiamine, 0.3mg niacin, 240mg potassium, 18.3mg sodium, 157mg calcium, 54mg magnesium, 2.2mg iron, 0.27mg Manganese, 0.35mg zinc, 0.15mg copper, 11mg phosphorus, 4.2μg selenium.

[0032] The specific preparation method of algae expansion culture solu...

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Abstract

The invention provides an isochrysis galbana propagation method. The highest propagation density of isochrysis galbana is increased by adding an algal beneficial bacterium separated from isochrysis galbana, and the plateau maintenance time of isochrysis galbana is prolonged. According to the method, one specific strain of the used phycomycetes beneficial bacteria screened from isochrysis galbana is an Alteromonas sp. NBU-A-0001 strain, and the preservation number of the phycomycetes beneficial bacteria is CCTCC M 2020010; according to the method disclosed by the invention, the propagation speed of isochrysis galbana is much higher than the microalgae culture speed of a conventional microalgae nutrient solution; the highest propagation density and the plateau period maintaining time are farlonger than those of a conventional culture nutrient solution, more microalgae can be supplied under the same water body condition, and meanwhile, due to the fact that the plateau period is usually not prone to decay, microalgae quantity control and production management in the actual shellfish large-scale production process are quite facilitated.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation as bait for aquaculture, and in particular relates to a method for propagating Isoflagellates, which can effectively improve the maximum reproduction density of Isoflagellates and the maintenance time of the plateau period. Background technique [0002] Isochrysis galbana is small in size, rich in polysaccharides, carotene and high-energy lipids, especially rich in unsaturated fatty acids DHA and EPA required for the growth and development of shellfish. It is an indispensable opening bait in the process of seed breeding. [0003] At present, domestic and foreign studies on the factors affecting the cultivation of Isoflagellates focus on the influence of environmental factors such as light, salinity, and temperature on the growth of Isoflagellates. The culture medium is organic and inorganic for the nutritional needs of microalgae. However, even if the suitable environmental and nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/38C12N1/12
Inventor 曹嘉懿孔周雁徐继林周成旭严小军
Owner NINGBO UNIV
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