Method for efficiently producing water-soluble beta-1, 3-glucan through chrysophyceae fermentation

Abstract

The invention relates to a method for efficiently producing water-soluble beta-1, 3-glucan through chrysophyceae fermentation. According to the method, chrysophyceae species of water-soluble beta-1, 3-glucan are produced by taking chrysophyceae with a high content of water-soluble beta-1, 3-glucan as a microorganism for fermentation culture. The method comprises the following steps of inoculatingchrysophyceae species into a fermentation basal culture medium, and feeding a supplementary culture medium in the fermentation culture process; and regulating the growth speed and the cell density ofchrysophyceae cells by regulating the type of a nitrogen source and the C / N ratio in the fermentation culture medium and maintaining the glucose concentration in the fermentation culture medium to bewithin a certain range, so that chrysophyceae fermentation culture liquid with high biomass concentration and high cell density is obtained, and the yield of the water-soluble beta-1, 3-glucan in thechrysophyceae cells is increased. On one hand, the problems that the content of beta-1, 3-glucan in an existing source of beta-1, 3-glucan is low, the extraction cost is high, and most beta-1, 3-glucan is insoluble in water are solved; and on the other hand, the problem that cell growth is inhibited when the content of storable polysaccharides in microalgae cells is increased in an existing nitrogen-limited or nitrogen-deficient mode is also solved.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and relates to a method for efficiently producing water-soluble β-1,3-glucan through golden algae fermentation. Background technique [0002] β-1,3-glucan is a kind of macromolecular polysaccharide widely present in microorganisms, plants and animals. The main chain structure is connected by β-1,3-glucosidic bonds, and usually contains different proportions and sizes due to different sources Branched chains connected by β-1,3, β-1,4 or β-1,6 bonds mainly exist in the form of cellular structural components such as cell walls. β-1,3-glucan is a high-efficiency biological responder with strong activity and low toxic and side effects. It not only has immune-stimulating effect, but also has anti-tumor and anti-infection activities. In the feed industry, water-soluble yeast β-1,3-glucan can be used as a feed additive, which can improve the non-specific and specific immunity of animals. At presen...

AI Technical Summary

Problems solved by technology

In terms of increasing the polysaccharide content of microalgae cells, the existing methods of nitrogen limitation or nitrogen starvation induction increase the content of storage polysaccharides (such as starch) in microalgae cells, and the cell growth is inhibited, and the polysaccharide production is difficult to increase, which is not suitable for industrial production.

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