Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preservation method of Isochrysis globosa

A technology of Isoflagellates and storage methods, which is applied in the field of microalgae cultivation, can solve the problems of low cell survival rate and short storage time, and achieve the effects of short flocculation time and improved antifreeze performance

Active Publication Date: 2021-07-13
XIAMEN CANCO BIOTECH CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current preservation method for Isoflagellates has a short preservation time, and the cell survival rate after long-term preservation is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] f / 2 medium: NaNO 3 75mg, NaSiO 3 9H 2 O 40mg, NaH 2 PO 4 2H 2 O 4.4mg, trace element solution 1mL, vitamin solution 1mL and the rest sterilized seawater, the total volume is 1L.

[0029] Trace element solution formula: CuSO 4 ·5H 2 O 10mg, ZnSO 4 ·7H 2 O 23mg, CoCl 6H 2 O12mg, MnCl 4H 2 O 178mg, NaMoO 4 2H 2 O 7.3mg, NaEDTA 4.35mg, FeCl 3 ·6H 2 O 3.9mg and the remainder pure water, total volume 1L;

[0030] Vitamin solution formula: B10.5mg, B120.5mg and the rest pure water, the total volume is 1L.

[0031] Seawater is filtered through sand, sedimentation, and absorbent cotton sieve, and then it is sterilized by high pressure and then used for later use. The salinity of the sterilized seawater is 28‰~33‰, and the pH value of the sterilized seawater is 7.8~8.1.

[0032] Add 10wt% dimethyl sulfoxide and 10wt% methanol to the f / 2 medium.

[0033] Ferric Chloride (FeCl 3 ·6H 2 O) configuration of the mother liquor: accurately weigh 0.5 g of ferric chloride...

Embodiment 2

[0041] f / 2 medium: NaNO 3 75mg, NaSiO 3 9H 2 O 40mg, NaH 2 PO 4 2H 2 O 4.4mg, trace element solution 1mL, vitamin solution 1mL and the rest sterilized seawater, the total volume is 1L.

[0042] Trace element solution formula: CuSO 4 ·5H 2 O 10mg, ZnSO 4 ·7H 2 O 23mg, CoCl 6H 2 O12mg, MnCl 4H 2 O 178mg, NaMoO 4 2H 2 O 7.3mg, NaEDTA 4.35mg, FeCl 3 ·6H 2 O 3.9mg and the remainder pure water, total volume 1L;

[0043] Vitamin solution formula: B10.5mg, B120.5mg and the rest pure water, the total volume is 1L.

[0044] Seawater is filtered through sand, sedimentation, and absorbent cotton sieves, and then high-pressure sterilized for use. The salinity of the sterilized seawater is 28‰~33‰, and the pH value of the sterilized seawater is 7.8~8.1.

[0045] Add 10wt% dimethyl sulfoxide and 10wt% methanol to the f / 2 medium.

[0046] Ferric Chloride (FeCl 3 ·6H 2 O) configuration of the mother liquor: accurately weigh 0.5 g of ferric chloride with an electronic balanc...

Embodiment 3

[0054] f / 2 medium: NaNO 3 75mg, NaSiO 3 9H 2 O 40mg, NaH 2 PO 4 2H 2 O 4.4mg, trace element solution 1mL, vitamin solution 1mL and the rest sterilized seawater, the total volume is 1L.

[0055] Trace element solution formula: CuSO 4 ·5H 2 O 10mg, ZnSO 4 ·7H 2 O 23mg, CoCl 6H 2 O12mg, MnCl 4H 2 O 178mg, NaMoO 4 2H 2 O 7.3mg, NaEDTA 4.35mg, FeCl 3 ·6H 2 O 3.9mg and the remainder pure water, total volume 1L;

[0056] Vitamin solution formula: B10.5mg, B120.5mg and the rest pure water, the total volume is 1L.

[0057] Seawater is filtered through sand, sedimentation, and absorbent cotton sieves, and then high-pressure sterilized for use. The salinity of the sterilized seawater is 28‰~33‰, and the pH value of the sterilized seawater is 7.8~8.1.

[0058] Add 10wt% dimethyl sulfoxide and 10wt% methanol to the f / 2 medium.

[0059] Ferric Chloride (FeCl 3 ·6H 2 O) configuration of the mother liquor: accurately weigh 0.5 g of ferric chloride with an electronic balanc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preserving Isochrysis globosa, which belongs to the technical field of microalgae cultivation. The method comprises the following steps: 1) Cultivating Isochrysis globosa with an f / 2 medium containing a mixed antifreeze agent. Obtaining Isochrysis globosa culture medium in the logarithmic growth phase; 2) Adding ferric chloride to the Isochrysis globosa culture fluid described in step 1) for flocculation to obtain flocculated Isochrysis globosa cells; 3) The flocculated Isochrysis globosa cells are collected, freeze-dried, and sealed for storage; the mixed antifreeze agent in step 1) includes dimethyl sulfoxide and methanol. Using the method of the present invention to preserve Isochrysis globosa, after one year of storage at room temperature, the survival rate of the Isochrysis globosa cells can reach 92%, which is far greater than the cell survival rate of conventional preservation methods.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, in particular to a method for preserving Isochrysis globosa. Background technique [0002] Isochrysis globosa belongs to Chrysophyta, Chrysophyceae, Isochrysis, Isochrysaceae, Isochrysis genus, single-celled marine planktonic algae, it is a good opening bait for marine animal larvae, and is widely used It can be used as basic bait for aquatic economic mollusc larvae. Isochrysis globosa contains more carotene and lutein, so it is brownish-yellow. It is a single-celled individual without a cell wall composed of cellulose and pectin. Flat dorsal and ventral surfaces, so it is oblong or rectangular in side view. Because the front end of the cell grows two equal-length tail whip-shaped flagella, it is named Isochrysis globosa. [0003] The concentration of microalgae is to concentrate freshly cultivated low-concentration algae liquid into high-concentration algae mud or algae cream by...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/04C12R1/89
CPCC12N1/04C12N1/12
Inventor 姜宗然袁学锋
Owner XIAMEN CANCO BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com