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Cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease

A technology for oil-producing microalgae and a culture method, which is applied in the field of culture to promote the rapid accumulation of oil-producing microalgae cells and oil, which can solve the problems of low oil content and difficult utilization, so as to increase oil content, promote growth and oil accumulation , the effect of simple operation

Inactive Publication Date: 2016-06-15
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heterotrophic cultures of algal cells grow faster but are low in lipids, which are often difficult to utilize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) The microalgae adopts Monoraphidium sp.FXY-10, using kuh1 medium, press 10gL -1 The amount of glucose is added as a carbon source, and the concentration of fulvic acid is 80mgL -1 ; Then the above-mentioned algae liquid was cultivated at 25°C under the condition of no light, and the single needle algae was cultivated to reach the late logarithmic growth phase, and the algae cell concentration reached 6.628gL at this time -1 , diluted to 2.3gL with light medium -1 As the seed solution in the light cultivation stage.

[0024] (2) In the autotrophic culture stage, kuh1 was used as the culture, and the pH value was adjusted to 6.8-7.0. Dissolve fulvic acid in ultrapure water and make 1000mgL -1 mother liquor, the concentration of fulvic acid in the medium is 5mgL -1 Then the above-mentioned algae liquid was cultivated under the induction of 25°C, light intensity 3500lux, cold light lamp continuous illumination and fulvic acid, and regularly sampled and measured biom...

Embodiment 2

[0026] (1) The microalgae adopts Monoraphidium sp.FXY-10, using kuh1 medium, press 10gL -1 The amount of glucose is added as a carbon source, and the concentration of fulvic acid is 80mgL -1 ; Then the above-mentioned algae liquid was cultivated at 25°C under the condition of no light, and the single needle algae was cultivated to reach the late logarithmic growth phase, and the algae cell concentration reached 6.628gL at this time -1 , diluted to 2.3gL with light medium -1 As the seed solution in the light cultivation stage.

[0027] (2) In the autotrophic culture stage, kuh1 was used as the culture, and the pH value was adjusted to 6.8-7.0. Dissolve fulvic acid in ultrapure water and make 1000mgL -1 mother liquor, the concentration of fulvic acid in the medium is 25mgL -1 Then the above-mentioned algae liquid was cultivated under the induction of 25°C, light intensity 3500lux, cold light lamp continuous illumination and fulvic acid, and regularly sampled every 2 days to ...

Embodiment 3

[0029] (1) The microalgae adopts Monoraphidium sp.FXY-10, using kuh1 medium, press 10gL -1 The amount of glucose is added as a carbon source, and the concentration of fulvic acid is 80mgL -1 ; Then the above-mentioned algae liquid was cultivated at 25°C under the condition of no light, and the single needle algae was cultivated to reach the late logarithmic growth phase, and the algae cell concentration reached 6.628gL at this time -1 , diluted to 2.3gL with light medium -1 As the seed solution in the light cultivation stage.

[0030] (2) In the autotrophic culture stage, kuh1 was used as the culture, and the pH value was adjusted to 6.8-7.0. Dissolve fulvic acid in ultrapure water and make 1000mgL-1 mother liquor, the concentration of fulvic acid in the medium is 125mgL -1 Then the above-mentioned algae liquid was cultivated under the induction of 25°C, light intensity 3500lx, cold light lamp continuous illumination and fulvic acid, and the biomass and oil content were mea...

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Abstract

The invention discloses a cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease. At first, under heterotrophic culture condition, the cells are stimulated to grow rapidly by utilizing fulvic acid; when the microalgae cells grow to the anaphase of logarithmic phase, cell concentration reaches 6.63g L<-1> at the moment; supernatant is removed through centrifugation so as to serve as a seed solution required in a subordinate phase. A 1000mgL<-1> fulvic acid mother solution is prepared by using pure water, and the fulvic acid mother solution is added in kuh1 culture mediums, so that the concentrations of the fulvic acidare 5, 25, 125, and 625mg L<-1> respectively. Then the obtained seed solution is added in the kuh1 culture mediums containing the fulvic acid of different concentrations, and the initial concentration of the seed solution is 2.3g L<-1>. Then the algae solution is subjected to continuous illumination cultivation, and sampling is performed every two other days for measuring biomass and grease content. The cultivation method disclosed by the invention is simple and feasible, and low in cost; the cultivation time is greatly shortened and the grease content is remarkably increased, and great significance is put forth for the industrialization of microalgae biodiesel production.

Description

technical field [0001] The invention specifically relates to a cultivation method for promoting rapid accumulation of oil-producing microalgae cells and oil, and belongs to the field of bioenergy. Background technique [0002] Biodiesel is a long-chain fatty acid methyl ester obtained by decomposing and esterifying biological oils as raw materials. It is an environmentally friendly and renewable energy that can replace fossil fuels. The raw materials of biodiesel come from vegetable oils (soybean oil, corn oil, etc.), animal oils (various animal fats), microalgae oils, and waste cooking oils (waste oil). [0003] At present, microalgae have been widely used as biodiesel feedstock in the world. Compared with other oil raw materials, microalgae has the advantages of fast cell growth, high oil content, and does not occupy arable land. There are three main culture methods for microalgae: autotrophic, heterotrophic and combined culture. At present, the industrial production of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/12C12R1/89
CPCC12N1/12C12P7/6436
Inventor 余旭亚车绕琼赵鹏徐军伟李涛
Owner KUNMING UNIV OF SCI & TECH
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