40 results about "Monoraphidium" patented technology

The invention provides a highly oil-containing monoraphidium and culture and application thereof, the monoraphidium strain is SS-B1, the classification and nomenclature of the monoraphidium is monoraphidium sp, the monoraphidium is preserved in CGMCC (China General Microbiological Culture Collection Center) on April 15, 2013, and the preservation number is CGMCC No.7479. The monoraphidium strain is able to tolerate high concentrations of CO2 and SO2, highly oil-containing biological matters can be obtained by light autotrophic growth by use of CO2 and SO2 containing waste gas or flue gas, the carbon fixation efficiency is high, cell total lipid content accounts for more than 40% of dry cell weight, and biodiesel can be produced.

The invention discloses acquisition and application of grease-producing monoraphidium LB50, and relates to screening and culture utilization technologies of the grease-producing monoraphidium. An algae species obtained by separating from field environment is subjected to 18S rDNA sequence analysis and morphological identification and is determined as a member of monoraphidium, and the sequence is shown as SEQ NO.1. The grease-producing monoraphidium LB50 is capable of bearing high-concentration sodium bicarbonate, is easy for open-type culture and is rich in grease; and when the growth and collection speed is kept at 22.2 g/m<2>*d, the grease content reaches 30.4% by dry weight of a cell, and aliphatic acid contained in grease contains octadecatrienoic acid and octadecatetraenoic acid which account 15% or more of aliphatic acid.

The invention discloses a method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil. The method comprises the following steps: diluting waste undecanted wine, taking the diluted waste undecanted wine as the basic culture medium for oil-producing monoraphidium; adding inorganic salts into the basic culture medium, adjusting the pH value by NaOH to produce a culture medium; adding 250 mL of the obtained culture medium into a conical flask (500 mL), sterilizing, adding oil-producing monoraphidium, culturing monoraphidium; collecting the cells by a centrifugal separation method, extracting the grease by a chloroform/methanol extraction liquid, and finally adding 2 mL of sulfuric acid-methanol solution (3%) into the extract to carry out methyl esterification reactions so as to obtain the biodiesel oil. The provided method is simple and practical, is capable of largely reducing the culture time of oil-producing monoraphidium and production cost, and obviously increasing the grease yield, and thus the production efficiency of biodiesel oil is greatly improved.

The invention relates to a method for producing microalgae grease by using flue gas. The method for the producing microalgae grease by using the flue gas comprises the following steps that firstly, amicroalgae culture medium and parachlorella kessleri FSH-Y3 or/and a scenedesmus obliquus FSH-Y2 seed solution are added into a photobioreactor, the pH value is adjusted to 10-12, the flue gas with CO2 content of 1v-5v% is introduced, and culture is carried out for a certain time; and then the pH value is adjusted to 8-10, a chlorella SF-B1 seed solution is introduced, at least one of a scenedesmus MH-04 seed solution a monoraphidium SS-B1 seed solution is introduced, mixed culture is carried out, the flue gas with CO2 content of 5v%-45v% is introduced, and the mixture is cultured to a stableperiod under the condition of continuous illumination, so that microalgae cells are obtained. The method for producing the microalgae grease by using the flue gas improves the tolerance and solubilityof a microalgae culture system to high-concentration CO2, improves the carbon fixation efficiency, obviously improves the harvest amount of the microalgae grease, simultaneously improves the tolerance to SOx and NOx in the flue gas, and can purify the flue gas.

The invention discloses an obtaining and an application of an oil-producing monoraphidium LB59, and relates to a screening and culturing technology of an oil-producing micro-alga. Through subjecting alga seeds separated from a field environment to 18SrDNA sequence analysis and morphological identification, the alga is determined to be a member of Monoraphidium, and the sequence is SEQ ID No.1. The oil-producing monoraphidium LB59 disclosed by the invention can tolerate high-concentrate sodium bicarbonate, is suitable for open type culture, is rich in grease, the grease content can reach 30.2% of dry cell weight under the condition that a growth and collection rate of 23.6g/m<2>d is held, and the fatty acid contains more than 20% of C-18 3-olefine acid and C-18 4-olefine acid.

The invention discloses a method for producing microalgae lipid by virtue of flue gas. The method comprises the following steps: (1) adding a microalgae medium and a parachlorella kessleri FSH-Y3 seed solution to a photobioreactor, regulating pH value of a culture system to 10-12, supplying the flue gas that a CO2 volume content is 1-5v%, and conducting culture for 2-5 days; and (2) regulating the pH value of the culture system to 8-10, inoculating the culture system with either a desmodesmus sp. MH-04 seed solution or a monoraphidium sp. SS-B1 seed solution or both of them, simultaneously, inoculating the culture system with an ankistrodesmus sp. SS-B7 and implementing mixed culture, and supplying the flue gas that a CO2 volume content is 5-45v%; implementing culture to a stationary phase under a continuous illumination condition, and collecting microalgae cells. According to the method provided by the invention, the tolerance and the solubility of the microalgae culture system to high-concentration CO2 are improved and a carbon fixation efficiency is improved, meanwhile, tolerance to SOx and NOx in the flue gas is improved, and the yield of the microalgae lipid is obviously improved.

The invention discloses a monoraphidium sp. strain SS-06 with high yield of starch and fat, wherein the classification name of the monoraphidium sp. strain SS-06 is Monoraphidium sp., and the monoraphidium sp. strain SS-06 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 24, 2015, and is assigned with the accession number of CGMCC No.10765. The bred monoraphidium sp. strain SS-06 has good application potential, is wide in environmental adaptation, and can produce starch and fat with high yield under the suitable condition, therefore, the different demands of the downstream production can be satisfied, and the application range of the monoraphidium sp. strain SS-06 is expanded.

The invention discloses a method for improving oil content of oil-producing microalgae based on fulvic acid. The method comprises the steps of preparing a culture medium based on fulvic acid; placing oil-producing monoraphidium in the culture medium to perform shaking culture; and preparing biodiesel. The fulvic acid improves microalgae growth rate and oil content, and the method has the advantages of being low in investment, simple to operate and high in efficiency. The method is favorable for urging the microalgae to absorb more nutrient more quickly and promoting development and growth of the microalgae. The activity of multiple synthetase is improved, the culture time of the microalgae is shortened, the oil content is greatly improved, and the method is of great significance to microalgae biodiesel production industrialization.

The invention relates to a method for producing microalgae grease by smoke. A culture medium and a scenedesmus obliquus FSH-Y2 or/ and Parachlorella kessleri FSH-Y3 seed solution are added into a photobioreactor to regulate pH to be 10-12, and light and dark alternation culture is carried out for a period of time; the pH is regulated to be 8-10, an Ankistrodesmus sp. SS-B7 seed solution inoculated, meanwhile, a Desmodesmus sp. MH-04 or/ and MonorapHidium sp SS-B1 seed solution is inoculated, and illumination culture is continued for a period of time; and then, a MonorapHidium sp SHJ-02 or/ andDesmodesmus sp. HCS-02 seed solution is inoculated, and light and dark alternation culture is carried out to a stable period under low illumination intensity so as to harvest microalgae cells. According to the method, the smoke is used for producing the microalgae grease, while hybrid bacterium pollution is inhibited, the tolerance and the solubleness of a microalgae culture system for high-concentration CO2 can be improved, carbon fixation efficiency is improved, and a harvest yield of the microalgae grease is obviously improved.

The invention discloses a wall-breaking and surfactant-assisted method used for extracting grease from microalgae containing oil. The wall-breaking and surfactant-assisted method comprises following steps: (1) pH value of a suspension liquid of microalgae containing oil is adjusted to be 7.5 to 14, and is subjected to hydro-thermal treatment; (2) after hydro-thermal treatment, enzymatic hydrolysis is carried out in the presence of enzymes; and (3) after enzymatic hydrolysis, a surfactant is added for reaction, and an organic solvent is added for extraction and separation of grease. Grease extraction rate of the wall-breaking and surfactant-assisted method is high; operation conditions are mild; nutritional ingredients of microalgae can be maintained effectively; using amount of the organic solvent is small; the wall-breaking and surfactant-assisted method is friendly to the environment, is low in energy consumption, and is suitable for nannochloropsis oculata, chlorella, diatom, scenedesmus, and monoraphidium komarkova-legnerova; and application range is wide.

The invention relates to a method for improving grease produced by microalgae. The method comprises the following steps of: firstly, adding a microalgae culture medium and a Parachlorella kessleri FSH-Y3 or/ and scenedesmus obliquus FSH-Y2 seed solution into a photobioreactor, regulating pH to be 10-12, and introducing gases of which the CO2 content is 1-5 v% to culture for certain time, wherein continuous illumination intensity is 8000-20000 Lux; and then, inoculating a MonorapHidium sp SHJ-02 or/ and Desmodesmus sp. HCS-02 seed solution, introducing gases of which the CO2 content is 5-45 v%,controlling a pH value to be 8-10, lowering the illumination intensity to 1000-5000 Lux, changing into light and dark alternation reaction, culturing to a stable period, and harvesting microalgae cells, wherein a light and dark period is 0.5-5s, and a light and dark time ratio is 1:1-5. According to the method, the tolerance and the solubleness of a microalgae culture system for high-concentration CO2 can be improved, and biomass and grease content are improved.

The invention discloses a method for producing lipid through mixed culture of microalgae. The method comprises the following steps: (1) adding a microalgae medium and a parachlorella kessleri FSH-Y3 seed solution to a photobioreactor, regulating pH value of a culture system to 10-12, supplying gas that a CO2 content is 1-5v%, and conducting culture for 2-5 days; (2) regulating the pH value of the culture system to 7-10, inoculating the culture system with desmodesmus sp. MH-04 and/or monoraphidium sp. SS-B1 seed solutions/seed solution and implementing mixed culture, and supplying gas that a CO2 content is 5-45v%; and (3) conducting the culture until the end of a stationary growth phase, and collecting microalgae cells. According to the method provided by the invention, the tolerance and the solubility of the microalgae culture system to high-concentration CO2 are improved, a carbon fixation efficiency is improved and the yield of the microalgae lipid is obviously improved; and the method is applicable to the production of biodiesel.

The invention discloses a method for producing lipid through mixed culture of microalgae. The method comprises the following steps: (1) adding a microalgae culture medium and scenedesmus obliqnus FSH-Y2 seed solution into a photo-biological reactor, and culturing for 2-5 days, wherein the pH value of a culture system is regulated to be 10-12, and the content of CO2 in the introduced gas is controlled to be 5v% or below; (2) adjusting the pH of the culture system to be 8-10, and inoculating with monoraphidium sp SS-B1 seed solution for mixed culture, wherein the content of CO2 in the introduced gas is 5v%-45v%; and (3) culturing till the stationary growth stage is finished, and harvesting microalgae cells, wherein scenedesmus obliqnus FSH-Y2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 11, 2012, and has the preservation number CGMCC No.6551, and monoraphidium sp SS-B1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 15, 2013, and has the preservation number CGMCC No.7479. With the adoption of the method, the tolerance and solubility of the microalgae culture system to high-concentration CO2 are improved, the carbon sequestration efficiency is improved, the harvest yield of the microalgae lipid is obviously improved, and the production of biodiesel is realized.

The invention relates to a method for cultivating monoraphidium sp. to produce biodiesel by using a walnut shell extracting solution, and belongs to the technical field of biodiesel. The method includes adding water in a walnut shell, boiling for more than 4h until the mass ratio of the liquid to the walnut shell in the system (g:g) is (4-32):1, conducting solid-liquid separation, utilizing the liquid as a basic medium, adding glucose in the basic medium, adjusting the pH of the basic medium to 6.8-7.0 with a sodium hydroxide solution and then conducting sterilization to obtain a monoraphidiumsp. medium; inoculating the monoraphidium sp. into a monoraphidium sp. medium and culturing at the temperature of 24-26 DEG C and the illumination intensity of 6000-7000 lux; measuring the biomass ofthe monoraphidium sp. under the culture conditions and utilizing an organic solvent to extract grease in monoraphidium sp. cells after the growth of the monoraphidium sp. for 60-80 hours; adding sulfuric acid-methanol into the grease for methyl esterification for 3-3.5 hours to obtain the biodiesel. According to the method, the walnut shell extracting solution is used as the basic medium, the glucose is added for concurrent culture of the monoraphidium sp., and the grease yield of the monoraphidium sp. is improved.

The application discloses a sewage deep-treatment device and method. The method utilizes rotary monoraphidium to carry out denitrification and phosphorus removal under a preset lighting condition. Themethod comprises the following steps: (1) sewage enters a reaction pool through a water inlet, a first aeration device supplies gas into the reaction pool and mixed liquid of the sewage treated by the reaction pool and microalgae enters a membrane pool; (2) a second aeration device supplies gas into the membrane pool, a membrane filtering component in the membrane pool separates the microalgae from the water, the treated water is discharged by a water discharging port on the membrane filtering component, one part of the mixed liquid of the microalgae and the sewage in the membrane pool returns into the reaction pool, and the other part of the mixed liquid is discharged by an algae port. The sewage deep-treatment device and method disclosed by the application have the beneficial effects that the concentrations of nitrogen and phosphorus in the water can be reduced to an extremely-low level in a short time, so that the risk of eutrophication of a water body collecting tail water from sewage plants is reduced.

The invention discloses a method for producing starch by Monoraphidium. The method comprises (1) preparing a microalgae seed liquid, wherein the microalgae is Monoraphidium sp. SS-06 and has a preservation number of CGMCC No. 10765, (2) inoculating an N element-rich microalgae culture medium with the Monoraphidium seed liquid, carrying out culture until a logarithmic growth phase, carrying out standing and sedimentation, discharging the supernatant and acquiring microalgae cells, (3) inoculating a low N element content microalgae culture medium with the microalgae cells, adding an ADP glucose pyrophosphatase inhibitor into the culture medium and carrying out continuous illumination culture until a stable period to obtain starch-rich microalgae cells. The method is conducive to proliferation of microalgae cells and accumulation of starch, can produce microalgae cells with high starch content and has the characteristics of simple processes, low cost and high yield.

The present invention relates to a method for promoting accumulation of microalgae oil under high-light and nitrogen-deficiency stress by using melatonin, and belongs to the field of microalgae biotechnology. A BG-11 basic medium using 10 g/L of glucose as a carbon source conducts heterotrophic culture of monoraphidium sp.QLY-1 at a temperature condition of 24-26 DEG C, algae cells are collected until the monoraphidium sp.QLY-1 grows to a late logarithmic phase, and a nitrogen-deficiency BG-11 culture medium dilutes the resuspended algae cells to 0.7 g/L as an inducing algae solution; absoluteethanol is used to prepare 10 mmol/L of a melatonin mother solution, the melatonin mother solution is added to the inducing algae solution to dilute a concentration of the melatonin into 10-80 [mu]mol/L, and the solution is subjected to induced culture at a temperature of 24-26 DEG C and an illumination intensity of 100-120 [mu]mol m<-2> s<-1>; and oil in the algae cells is extracted with an organic solvent. The method is simple in operation, can shorten induction cycle of the algae cells, increases content of oil, ensures growth of the microalgae, and can solve problems of biomass reduction,low oil content, etc. in an induction process of microalgae industrialization.