Method for promoting accumulation of microalgae oil under high-light and nitrogen-deficiency stress by using melatonin

A technology of oil accumulation and melatonin, applied in the direction of microbial-based methods, oil/fat production, oil/fat refining, etc., can solve the problem of increasing the cost of microalgae to produce biodiesel, reducing the yield of microalgae biomass, algae cell Low oil content and other problems, to achieve the effect of simple and convenient process, small molecular weight, and increase the accumulation of oil

Inactive Publication Date: 2019-09-13
KUNMING UNIV OF SCI & TECH
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AI Technical Summary

Benefits of technology

This patented technology allows people skilled at this field to study how different types of chemicals affect algae's ability to grow or produce certain compounds from their environment. It provides technical benefits such as controlling cell division, promoting photosynthetic activity during starvation conditions, increasing nutrients production when exposed to extreme environmental factors like heat waves, reducing carbon dioxide levels (CO2), enhancing drought tolerance, prevention against disease-causing agents, stimulating vegetative cells, creating better food sources through reduced fertility rates caused by excessive water supply, etc., resulting in more efficient crop yields over time.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an effective way to produce fuel that uses sunlight or other resources instead of fossil fuels like coal due to its ability to grow faster than traditional crops while also being able to store more valuable products during periods when they're no longer needed.

Method used

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  • Method for promoting accumulation of microalgae oil under high-light and nitrogen-deficiency stress by using melatonin

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: A kind of method that utilizes melatonin to promote microalgae oil accumulation under the stress of high light and nitrogen deficiency, the specific steps are as follows:

[0029] (1) Preparation of induced algae liquid: Under the condition of temperature of 25°C, the BG-11 basal medium with 10g / L glucose as the carbon source is used for heterotrophic cultivation of Monostipella, and the growth of Monostipma to the late logarithmic growth phase Collect the algal cells, dilute and resuspend the algal cells to 0.7g / L with nitrogen-deficient BG-11 medium as the induced algal liquid;

[0030] (2) Induce algae cells to accumulate oil: prepare 10mmol / L melatonin mother liquor with absolute ethanol, add melatonin mother liquor to step (1) in the induced algae liquor to dilute melatonin concentration to be 20 μ mol / L, and Placed at a temperature of 25°C and a light intensity of 105 μmol m -2· the s -1 Under the conditions, the cold light lamp is continuously ill...

Embodiment 2

[0032] Embodiment 2: A kind of method that utilizes melatonin to promote microalgae lipid accumulation under high light and nitrogen deficiency stress, concrete steps are as follows:

[0033](1) Preparation of induced algae liquid: Under the condition of temperature of 24°C, the BG-11 basal medium with 10g / L glucose as the carbon source is used for heterotrophic cultivation of Monostipella, and the growth of Monostipma to the late logarithmic growth phase Collect the algal cells, dilute and resuspend the algal cells to 0.7g / L with nitrogen-deficient BG-11 medium as the induced algal liquid;

[0034] (2) Induce algae cells to accumulate oil: prepare 10mmol / L melatonin mother liquor with absolute ethanol, add melatonin mother liquor to step (1) in the induced algae liquor to dilute melatonin concentration to be 40 μ mol / L, and Placed at a temperature of 24°C and a light intensity of 110 μmol m -2· the s -1 Under the conditions, the cold light lamp is continuously illuminated t...

Embodiment 3

[0036] Embodiment 3: A kind of method that utilizes melatonin to promote microalgae lipid accumulation under high light and nitrogen deficiency stress, concrete steps are as follows:

[0037] (1) Preparation of induced algae liquid: Under the condition of temperature of 25°C, the BG-11 basal medium with 10g / L glucose as the carbon source is used for heterotrophic cultivation of Monostipella, and the growth of Monostipma to the late logarithmic growth phase Collect the algal cells, dilute and resuspend the algal cells to 0.7g / L with nitrogen-deficient BG-11 medium as the induced algal liquid;

[0038] (2) Induce algae cells to accumulate oil: prepare 10mmol / L melatonin mother liquor with absolute ethanol, add melatonin mother liquor to step (1) in the induced algae liquid to dilute melatonin concentration to be 80 μ mol / L, and Placed at a temperature of 26°C and a light intensity of 115 μmol m -2 ·s -1 Under the conditions, the cold light lamp is continuously illuminated to i...

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Abstract

The present invention relates to a method for promoting accumulation of microalgae oil under high-light and nitrogen-deficiency stress by using melatonin, and belongs to the field of microalgae biotechnology. A BG-11 basic medium using 10 g/L of glucose as a carbon source conducts heterotrophic culture of monoraphidium sp.QLY-1 at a temperature condition of 24-26 DEG C, algae cells are collected until the monoraphidium sp.QLY-1 grows to a late logarithmic phase, and a nitrogen-deficiency BG-11 culture medium dilutes the resuspended algae cells to 0.7 g/L as an inducing algae solution; absoluteethanol is used to prepare 10 mmol/L of a melatonin mother solution, the melatonin mother solution is added to the inducing algae solution to dilute a concentration of the melatonin into 10-80 [mu]mol/L, and the solution is subjected to induced culture at a temperature of 24-26 DEG C and an illumination intensity of 100-120 [mu]mol m<-2> s<-1>; and oil in the algae cells is extracted with an organic solvent. The method is simple in operation, can shorten induction cycle of the algae cells, increases content of oil, ensures growth of the microalgae, and can solve problems of biomass reduction,low oil content, etc. in an induction process of microalgae industrialization.

Description

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Claims

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Application Information

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Owner KUNMING UNIV OF SCI & TECH
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