Method for promoting accumulation of microalgae oil under high-light and nitrogen-deficiency stress by using melatonin

Abstract

The present invention relates to a method for promoting accumulation of microalgae oil under high-light and nitrogen-deficiency stress by using melatonin, and belongs to the field of microalgae biotechnology. A BG-11 basic medium using 10 g/L of glucose as a carbon source conducts heterotrophic culture of monoraphidium sp.QLY-1 at a temperature condition of 24-26 DEG C, algae cells are collected until the monoraphidium sp.QLY-1 grows to a late logarithmic phase, and a nitrogen-deficiency BG-11 culture medium dilutes the resuspended algae cells to 0.7 g/L as an inducing algae solution; absoluteethanol is used to prepare 10 mmol/L of a melatonin mother solution, the melatonin mother solution is added to the inducing algae solution to dilute a concentration of the melatonin into 10-80 [mu]mol/L, and the solution is subjected to induced culture at a temperature of 24-26 DEG C and an illumination intensity of 100-120 [mu]mol m<-2> s<-1>; and oil in the algae cells is extracted with an organic solvent. The method is simple in operation, can shorten induction cycle of the algae cells, increases content of oil, ensures growth of the microalgae, and can solve problems of biomass reduction,low oil content, etc. in an induction process of microalgae industrialization.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows people skilled at this field to study how different types of chemicals affect algae's ability to grow or produce certain compounds from their environment. It provides technical benefits such as controlling cell division, promoting photosynthetic activity during starvation conditions, increasing nutrients production when exposed to extreme environmental factors like heat waves, reducing carbon dioxide levels (CO2), enhancing drought tolerance, prevention against disease-causing agents, stimulating vegetative cells, creating better food sources through reduced fertility rates caused by excessive water supply, etc., resulting in more efficient crop yields over time.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an effective way to produce fuel that uses sunlight or other resources instead of fossil fuels like coal due to its ability to grow faster than traditional crops while also being able to store more valuable products during periods when they're no longer needed.

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