31results about How to "Guaranteed biomass" patented technology

The invention discloses a treatment method of antibiotic fermenting bacterial residues. The treatment method comprises the following steps: uniformly mixing the fermenting bacterial residues of lincomycin hydrochloride and gentamicin with water, and preparing a suspension; performing anaerobic fermentation treatment on the suspension. The treatment method is an efficient fermentation treatment process of the fermenting bacterial residues of lincomycin hydrochloride and gentamicin and belongs to the technical field of resource utilization of the bacterial residues. By adopting the method disclosed by the invention, the toxicity, the harm and an inhibition effect of residual antibiotics to fermenting bacteria in an anaerobic fermentation process of the traditional antibiotic bacterial residues are overcome, the problems of blockage, short flow and the like which are produced when a traditional filler is fed are simultaneously avoided, the biomass of a hydrolysis acidification pool and an L-fermentation tank is ensured, the gas production is improved and the method further has the advantages of low treatment cost, easiness in control of operation process, high load of a reactor matched with the method, small occupied area and the like.

The invention belongs to the technical field of environmental engineering sewage treatment, and in particular relates to an integral air-lift circumfluent suspended filler dispersed sewage biological treatment device. The device integrates four functional units, which respectively are an anoxia biological regulation tank, a suspended filler biological reaction tank, a secondary sedimentation tank and a mud storage tank. The round vertical flow type secondary sedimentation tank is arranged at the middle part of a reactor, other three functional units are located around the central secondary sedimentation tank in radiating way, and the appearance of the entire device is in cylindrical structure. Rural dispersed domestic sewage enters into the anoxia biological regulation tank uniformly through a pipeline, is subject to hydrolyzation and acidification, lifted into the suspended filler biological reaction tank by a sewage air-lifter, spherical suspended filler with filling ratio of 40-50% is thrown in the suspended filler biological reaction tank, enters into the central secondary sedimentation tank through a barrier mesh at the drain hole, sediment water and mud are separated, mud is collected by a triangular-notch weir and water is discharged via a water collecting sump. Nitrification liquid in the suspended filler biological reaction tank reflows into the anoxia biological regulation tank via a nitrification liquid air-lifter for denitrification by utilizing influent organic carbon source. Sediment mud in the secondary sedimentation tank enters into the mud storage tank via a mud air-lifter, and the mud drainage period is long. The invention utilizes the anoxia biological regulation tank to achieve functions of removing organic substances and denitrifying; the compact integral structure occupies a small area of land; system equipments are few, thus investment and running cost are little; the invention is suitable for rural dispersed domestic sewage treatment.

The invention relates to a method for promoting accumulation of heterotrophic microalgae oil through combination of melatonin and nitrogen deficiency, and belongs to the field of microalgae bio-technology. The method comprises the following steps: implementing heterotrophic culture of monoraphidium with the participation of a BG-11 basal culture medium which takes 10g/L glucose as a carbon sourceat a temperature of 24-26 DEG C, collecting algae cells in the later period of logarithmic phase of the monoraphidium, and diluting and re-suspending the algae cells via a BG-11 culture medium with nitrogen deficiency so as to obtain 0.85-1g/L inducing algae liquid; preparing 100[mu]mol/L melatonin mother liquid via absolute ethyl alcohol, adding the melatonin mother liquid to the inducing algae liquid so that melatonin concentration is diluted to 0.1-10[mu]mol/L, and implementing inducing culture at a temperature of 24-26 DEG C and under a light condition; and extracting oil in the algae cells via an organic solvent. The method provided by the invention is simple to operate, an algae cell inducing cycle can be shortened, an oil content can be improved and microalgae growth can be guaranteed; and such problems as biomass dropping, low oil content and the like in a microalgae industrial inducing process can be solved.

The invention belongs to the technical field of microorganisms, and relates to a method for producing inonotus obliquus exopolysaccharides through submerged fermentation, particularly to a method for industrial-scale production of the inonotus obliquus exopolysaccharides. The method comprises the steps as follows: activated inonotus obliquus strains are inoculated into a seed tank for propagation, then propagated liquid seeds are inoculated into a specially-made polysaccharide-producing liquid culture medium for fermentation, and a fermentation liquid is separated to extract the exopolysaccharides. According to the method, research is conducted to overcome defects in the prior art, on the premises that a fermentation culture medium (especially a carbon source) is continuously optimized through small and medium tests and the biomass and the exopolysaccharides are increased, the problems of fermentation process parameter control, extracting equipment model selection, optimization and the like in the process of enlargement from the small tests to industrial production are solved, the strains are successfully inoculated into fermentation tanks of 10-20 t finally for industrial production, the high-quality inonotus obliquus polysaccharides are produced smoothly, and the continuous, automatic and industrial production is actually realized.

A phenol inhibition-coupled low temperature short-cut denitrification method belongs to the field of sewage treatment. The pre-denitrification operation mode of a sequencing batch reactor (SBR) is adopted, raw water and phenol compound are sufficiently utilized as carbon source, and additional carbon source is not added. When the temperature is linearly decreased to 15 DEG C from 25 DEG C and thephenol concentration is 0mg/L to 30mg/L, the system does not discharge sludge; when the phenol concentration is 60mg/L, the sludge concentration is kept at 4000mg/L; and when the phenol concentrationis 90mg/L, the sludge concentration is kept at 5000mg/L. The increase of the nitrite accumulation rate under low-concentration phenol is slow, and the nitrite accumulation rate is less than 5 percentwhen the phenol concentration is 0mg/L, reaches 22 percent at the final stage when the phenol concentration is 30mg/L, and is 43 percent at the final stage when the phenol concentration is 60mg/L; the affection of high-concentration phenol on the composition of system sludge is great, and the nitrite accumulation rate reaches 91 percent and is stably kept at more than 90 percent at the final stage. The method is applicable to industrial wastewater treatment in cold northern regions and can be adopted as a technical reference for the inhibition of the toxicity of phenolic compounds in urban sewage.

The invention discloses a penicillin waste water biological enhancement treatment device and method, and belongs to the technical field of waste water treatment and environmental protection. According to the device, the characteristics of multiple sewage treatment devices such as a sequencing batch type reactor, a membrane bioreactor and a moving bed bio-membrane reactor are integrated; the device mainly comprises a hydrolytic acidification area, a moving bed bio-membrane area provided with suspended filler, and a deep treatment area provided with a membrane assembly and suspended filler. The hydrolytic acidification area is used for pretreating waste water, improving biodegradability of the penicillin waste water and degrading a part of pollutants, and the deep treatment area is a main penicillin waste water biological treatment system. According to the penicillin waste water biological enhancement treatment device and method, the treatment efficiency of the penicillin waste water can be effectively improved, the occupied area can be reduced, the effluent quality can be kept stable, the service life of an aerating device and the membrane assembly can be prolonged, maintenance and operating cost can be reduced, and the penicillin waste water biological enhancement treatment device and method are especially suitable for treating the antibiotic waste water with large water quality and amount changes.

The invention relates to a moving bed biofilm reactor deposition integrated denitrification reaction tower. The moving bed biofilm reactor deposition integrated denitrification reaction tower comprises a moving bed biofilm reactor and a deposition pool arranged at the periphery of the side wall of the moving bed biofilm reactor, the moving bed biofilm reactor is loaded with suspended filler, an aeration device is arranged at the edge of the bottom, a jet flow water distributor is further arranged in the center of the bottom, a water inlet is formed, a deposition pool inlet is further formed inthe side wall, a sludge reflowing opening is formed below the deposition pool, sludge flowing out of the sludge reflowing opening is mixed with inlet water, is afterwards jetted out of the edge of the bottom of the moving bed biofilm reactor through the jet flow water distributor and is then in contact with the suspended filler to form mixed liquid, and then through upward lifting of the aerationdevice, a hydraulic circulation denitrification environment of aerobic and anoxic alternating is formed. The deposition function is integrated while efficient denitrification is achieved, the technical problems that the suspended filler in the tower-type denitrification reactor is easily lost and running and flowing are not uniform are solved, the structure is compact, the occupied area is small,running is simple, timely power-free sludge reflowing is achieved, and energy is obviously saved.

The invention discloses an MBBR (Moving Bed Biofilm Reactor) used for electroplating wastewater treatment. The MBBR comprises a reaction tank and a sedimentation tank, wherein the water inlet end of bottom of the reaction tank is equipped with a water inlet tube, a gas inlet tube and at least one submersible mixer; the bottom of the reaction tank is equipped with an aeration tube and a filler sedimentation ditch with a filler discharge tube; the inner part of the filler sedimentation ditch is equipped with a spiral conveyor; the water outlet end of the upper part of the reaction tank is equipped with a rotary scraper plate and a screen; the inner part of the reaction tank is added with suspended filler; the water inlet end of the sedimentation tank is equipped with a guide slot; the upperpart of the tank body is equipped with a water outlet weir and a water outlet tube; and the bottom of the tank body is equipped with a sludge discharge tube and a sludge reflux tube. The rotary scraper plate and the submersible mixer are arranged in the reactor, so that a circulating flowing state of the suspended filler is guaranteed; the filler sedimentation ditch is arranged for timely discharging scaled filler, so that the biological treatment effect, on electroplating wastewater with poor biodegradability and high salinity, of the MBBR process can be effectively strengthened.

The invention discloses a method for producing xylaria nigripes exopolysaccharides through submerged fermentation. The method comprises the steps as follows: activated xylaria nigripes strains are inoculated into a seed tank for propagation, then propagated liquid seeds are inoculated into a specially-made polysaccharide-producing liquid culture medium for fermentation, and a fermentation liquid is separated to extract the exopolysaccharides. According to the method, research is conducted to solve the technical problems encountered during amplification from small tests to industrial production, on the premises that a fermentation culture medium (especially a carbon source) is continuously optimized through small and medium tests and the biomass and the exopolysaccharides are increased, the problems of fermentation process parameter control, extracting equipment model selection, optimization and the like during enlargement are solved, the strains are successfully inoculated into fermentation tanks of 10-20 t finally for industrial production, the high-quality xylaria nigripes exopolysaccharides are produced smoothly, and the continuous, automatic and industrial production is actually realized.

The invention belongs to the technical field of microbes, particularly relates to method for producing marasmius androsaceus (L. ex Fr.) Fr. extracellular polysaccharide through submerged liquid state fermentation, and in particular to a production method for marasmius androsaceus (L. ex Fr.) Fr. extracellular polysaccharide in an industrialization scale. The method comprises the following steps: conducting expanded matching on activated marasmius androsaceus (L. ex Fr.) Fr. strains through inoculating into a seeding tank, inoculating the liquid seeds subjected to expanded matching to a tailor-made liquid culture medium capable of producing polyose to conduct fermentation, separating the fermentation liquor, and extracting the extracellular polysaccharide. The invention aims to conduct a study on the deficiencies in the prior art, on the basis of continuously optimizing a fermentation culture medium (in particular to a carbon source), improving the biomass and the extracellular polysaccharide in a small-scale test and a pilot scale test, the problems such as parameter control in the fermentation process, extracting equipment lectotype, optimization and the like in the process from the small-scale test to the industrialized production are solved, finally, the marasmius androsaceus (L. ex Fr.) Fr. strains are grafted onto 10-20 t fermentation tanks to conduct industrialized production, and the marasmius androsaceus (L. ex Fr.) Fr. polyose with high quality is produced smoothly, so that the production with continuity, automatization, and industrialization is realized really and truly.

The invention belongs to the technical field of environmental engineering sewage treatment, and particularly relates to an integrated sewage treatment system. The device integrates four functional units including an anoxic biological regulation tank, a suspended filler biological reaction tank, a secondary sedimentation tank and a sludge storage tank. A circular vertical flow type secondary sedimentation tank is arranged in the middle of the reactor, wherein the other three functional units are distributed around the central secondary sedimentation tank in a radial shape, and the whole device is of a cylindrical structure in appearance. Rural decentralized domestic sewage is uniformly distributed into the anoxic biological regulation tank through a pipeline, is hydrolyzed and acidified, and then is lifted by the sewage air lifter to enter the suspended filler biological reaction tank; wherein the spherical suspended filler with the filling ratio of 40-50% is put, and the sewage enters the central secondary sedimentation tank through the intercepting net at the overflow hole; finally, the sediment is separated from mud and water, collected by a triangular weir and discharged by a water collecting tank; the nitration liquid of the suspended filler biological reaction tank flows back to the anoxic biological regulation tank through a nitration liquid air lifter, and denitrification nitrogen removal is carried out by utilizing an inlet water organic carbon source.

The invention discloses a shellfish compound feed based on enteromorpha and a preparation method thereof. The shellfish compound feed comprises 100-120 parts by weight of enteromorpha dry powder, 120-180 parts by weight of distilled water, 3-5 parts by weight of probiotics, 15-20 parts by weight of fish meal, 5-10 parts by weight of fish oil, 1.5-2 parts by weight of sodium alginate and 1-1.5 parts by weight of calcium chloride. The preparation method comprises the following steps: cleaning enteromorpha to remove impurities, and then drying the cleaned enteromorpha; grinding the dried enteromorpha to obtain enteromorpha dry powder; weighing the raw materials, and uniformly mixing and stirring to obtain a mixed material; and storing the mixed material in a sealed oxygen-free environment for 48-72 hours, and keeping the temperature at 32-37 DEG C to prepare the shellfish compound feed. Compared with the prior art, the method has the advantages that the enteromorpha is used as a main component of the shellfish compound feed, the added value of the enteromorpha is increased, resource utilization of the enteromorpha is realized, and the environment is protected. The method is simple to operate, no excessive complex equipment is aquired, feasibility is high and rapid large-scale production can be realized.

The invention discloses a denitrification method of an improved SBR (sequencing batch reactor). According to the method, the advantages of SBR and anaerobic ammonia oxidation are combined, anaerobic ammonia oxidizing bacteria are added into an SBR on the basis of a traditional SBR process, and anoxic and aerobic alternate circulation of the reactor is controlled in an intermittent aeration mode. Through multiple alternation of an anoxic environment and an aerobic environment in an operation period, accumulation of nitrite is effectively realized, and sufficient reaction substrates are providedfor an anaerobic ammonia oxidation process, so that denitrification of the improved SBR is realized. According to the method disclosed by the invention, the defects of an SBR process and an anaerobicammonia oxidation process are effectively overcome; the SBR process and the anaerobic ammonia oxidation process are coupled to realize a good denitrification function; the energy consumption is reduced, an organic carbon source does not need to be additionally added, the operation cost is reduced, the biomass in the reactor can be ensured by the operation method, and microorganisms in each stagecan fully exert own advantages and functions. The denitrification method has obvious advantages in treating sewage with high ammonia nitrogen and low carbon nitrogen ratio.