31results about How to "Guaranteed biomass" patented technology

The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing exopolysaccharide of Inonotus obliquus by submerged liquid fermentation. Specifically, it is an industrial-scale production method of Inonotus obliquus exopolysaccharide. The method is to expand the activated Inonotus obliquus strains by inserting them into the seed tank, and then add the expanded liquid The seeds are inserted into a special polysaccharide-producing liquid medium for fermentation, and then the fermentation broth is separated to extract exopolysaccharides. The present invention studies the above-mentioned deficiencies in the prior art, on the basis of continuing to optimize the fermentation medium (especially the carbon source) and improving the biomass and exopolysaccharide in the small test and the pilot test, it solves the problem of scaling up from small test to industrialized production The difficulties encountered in the fermentation process parameter control, extraction equipment selection, optimization, etc., were finally successfully grafted into a 10-20t fermenter for industrial production, and successfully produced high-quality Inonotus obliquus polysaccharides, truly realizing continuous, Automated, industrialized production.

The invention discloses an integrated bubbleless aeration membrane bioreactor. A hollow-fiber curtain type membrane (7) is vertically placed in a biochemical reactor (6), the upper and the lower ends of the hollow-fiber curtain type membrane (7) are respectively fixed in the sidewalls of one-way PVC (polyvinyl chloride) tubes by resin, the PVC tube on the lower end of the hollow-fiber curtain type membrane (7) is connected with an intake tube, the PVC tube on the upper end of the hollow-fiber curtain type membrane (7) is connected with an exhaust tube, the intake tube is connected with an air compressor (3) through an air rotameter (5), and the exhaust tube leads into the air via a gas fine-regulating valve (16). Because the integrated bubbleless aeration membrane bioreactor adopts the hollow-fiber curtain type membrane structure, the oxygen utilization rate approximates 100 percent, the air is inputted from the air compressor via the lower end of the hollow-fiber curtain type membrane, and flows in the hollow-fiber membrane tube cavities, the oxygen in the tube cavities is pushed by the difference between the oxygen partial pressures of both sides of the membrane to directly disperse into the external biomembrane via the membrane wall or micropores on the membrane wall, consequently, the oxygen transfer efficiency is greatly increased, the requirement of the microbiological oxidative degradation of organic pollutants on oxygen consumption is met, and moreover, the gas fine-regulating valve after the exhaust tube can be utilized to effectively and stably control the pressure and flow velocity of gas, so that the aeration quantity can be conveniently and accurately controlled.

A single-stage AOA-SBBR device and method for strengthening deep denitrification and phosphorus removal of endogenous denitrification sewage belongs to the technical field of sewage biological treatment. The method is as follows: the sewage enters the single-stage AOA-SBBR strengthened endogenous denitrification reactor through the water inlet pump, and in the anaerobic stirring stage, the phosphorus accumulating bacteria and polysaccharide bacteria make full use of the organic carbon source in the raw water to store the internal carbon source, at this time Accompanied by the release of phosphorus by phosphorus-accumulating bacteria; during the aeration and stirring stage, nitrifying bacteria release NH in raw water 4 + ‑N to NO 3 ‑ ‑N, accompanied by the aerobic phosphorus uptake by phosphorus accumulating bacteria; in the stage of anoxic stirring, NO 3 ‑ ‑N is used by phosphorus-accumulating bacteria and glycan bacteria to achieve endogenous denitrification. In the present invention, by adding the nitrifying bacteria filler, the anoxic section is rear-positioned under the premise of ensuring the biomass of the nitrifying bacteria, which effectively solves the competition between the denitrifying bacteria, the phosphorus accumulating bacteria and the polysaccharide bacteria for the substrate, and can save energy, On the basis of making full use of carbon sources, the synchronous deep denitrification and phosphorus removal of sewage can be realized.

The invention discloses a method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation, particularly an industrial-scale production method of Antrodia camphorata extracellular polysaccharides. The method comprises the following steps: inoculating activated Antrodia camphorata strain into a seed tank to perform amplification culture, inoculating the liquid seed into a purpose-made polysaccharide-producing liquid culture medium to perform fermentation, separating the fermentation liquid, and extracting the extracellular polysaccharides. In order to provide the yield of the extracellular polysaccharides, the method optimizes the formula of the fermentation culture medium, and adopts the compound formula against the carbon source part, thereby ensuring the biomass of the earlier-stage thallus and also ensuring the yield of the later-stage metabolites. The method can be used for preparing high-quality polysaccharides with different contents, and the polysaccharide content can be 20-50%.

The invention belongs to the technical field of environmental engineering sewage treatment, and in particular relates to an integral air-lift circumfluent suspended filler dispersed sewage biological treatment device. The device integrates four functional units, which respectively are an anoxia biological regulation tank, a suspended filler biological reaction tank, a secondary sedimentation tankand a mud storage tank. The round vertical flow type secondary sedimentation tank is arranged at the middle part of a reactor, other three functional units are located around the central secondary sedimentation tank in radiating way, and the appearance of the entire device is in cylindrical structure. Rural dispersed domestic sewage enters into the anoxia biological regulation tank uniformly through a pipeline, is subject to hydrolyzation and acidification, lifted into the suspended filler biological reaction tank by a sewage air-lifter, spherical suspended filler with filling ratio of 40-50%is thrown in the suspended filler biological reaction tank, enters into the central secondary sedimentation tank through a barrier mesh at the drain hole, sediment water and mud are separated, mud iscollected by a triangular-notch weir and water is discharged via a water collecting sump. Nitrification liquid in the suspended filler biological reaction tank reflows into the anoxia biological regulation tank via a nitrification liquid air-lifter for denitrification by utilizing influent organic carbon source. Sediment mud in the secondary sedimentation tank enters into the mud storage tank viaa mud air-lifter, and the mud drainage period is long. The invention utilizes the anoxia biological regulation tank to achieve functions of removing organic substances and denitrifying; the compact integral structure occupies a small area of land; system equipments are few, thus investment and running cost are little; the invention is suitable for rural dispersed domestic sewage treatment.

The invention discloses a treatment method of antibiotic fermenting bacterial residues. The treatment method comprises the following steps: uniformly mixing the fermenting bacterial residues of lincomycin hydrochloride and gentamicin with water, and preparing a suspension; performing anaerobic fermentation treatment on the suspension. The treatment method is an efficient fermentation treatment process of the fermenting bacterial residues of lincomycin hydrochloride and gentamicin and belongs to the technical field of resource utilization of the bacterial residues. By adopting the method disclosed by the invention, the toxicity, the harm and an inhibition effect of residual antibiotics to fermenting bacteria in an anaerobic fermentation process of the traditional antibiotic bacterial residues are overcome, the problems of blockage, short flow and the like which are produced when a traditional filler is fed are simultaneously avoided, the biomass of a hydrolysis acidification pool and an L-fermentation tank is ensured, the gas production is improved and the method further has the advantages of low treatment cost, easiness in control of operation process, high load of a reactor matched with the method, small occupied area and the like.

The invention provides an integrated treatment device and method for low concentration sewage, and relates to a sewage treatment device and method, aiming to solve the technical problem that the existing anaerobic expanded bed reactor has low efficiency for treating nitrogen and phosphorus and easily causes sludge to lose. The device comprises a bamboo-shaped aerobiotic external cylinder, an anaerobic internal cylinder, a three-phase separation cylinder, a bamboo-shaped hollow cylinder, a water distributor, a gas collecting cover, a gas delivery pipe, a mud discharging hopper, a mud discharging pipe, and an S-shaped connecting pipe, wherein the anaerobic internal cylinder is arranged in the bamboo-shaped aerobiotic external cylinder, and the bottom of the three-phase separation cylinder is connected with the top of the anaerobic internal cylinder; the bamboo-shaped hollow cylinder is arranged in the anaerobic internal cylinder, a filler is filled in the bamboo-shaped aerobiotic external cylinder, and a mixture of anaerobic granular sludge and the filler is filled in the bamboo-shaped hollow cylinder. The method comprises the following steps of: feeding sewage into the anaerobic internal cylinder, reacting with anaerobic granular sludge in the hollow cylinder, rising into the three-phase separation cylinder for separation, dropping outlet water in the bamboo-shaped aerobiotic external cylinder for aerobic treatment, and completing treatment. The device and method is used for treating low concentration sewage.

The invention belongs to the technical field of microbes, and in particular relates to a method for producing exopolysaccharides from the umbrella of Anluo Xiaopi by submerged liquid fermentation. Specifically, it is an industrial-scale production method of exopolysaccharides of Pygmy Anluo. The liquid seeds are inserted into a special polysaccharide-producing liquid medium for fermentation, and then the fermentation broth is separated to extract exopolysaccharides. The present invention studies the above-mentioned deficiencies in the prior art, on the basis of continuing to optimize the fermentation medium (especially the carbon source) and improving the biomass and exopolysaccharide in the small test and the pilot test, it solves the problem of scaling up from small test to industrial production The difficulties encountered in the fermentation process parameter control, extraction equipment selection, optimization, etc., were finally successfully grafted into a 10-20t fermenter for industrial production, and successfully produced high-quality Anluo Xiaopi polysaccharides, truly realizing the continuous , automated, industrialized production.

The invention provides a method for quickly starting anaerobic ammonium oxidation in an upflow packed bed reactor. An upflow packed bed is taken as a reactor; a reaction zone and a precipitation zone are arranged in the reactor; a suspended filler which has a high specific surface area and density close to that of water and is difficult to block is selected as a reaction filler; activated sludge in a membrane bioreactor (MBR) tank in a process for treating anaerobic ammonium oxidation sludge and refuse landfill percolate is subjected to mixed inoculation; and the temperature of the reaction zone is controlled to be 32+ / -1 DEG C by a self-temperature control heating zone with a temperature control system. The suspended filler is added, so that flocculent sludge is retained in the reactor, biomass in the reactor is ensured, and an anaerobic ammonium oxidation process is quickly started; and the activated sludge in the MBR tank in the process for treating the anaerobic ammonium oxidation sludge and the refuse landfill percolate is subjected to mixed inoculation, so that the acclimation of the activated sludge in the MBR tank is promoted, acclimation time is shortened, and the starting speed is increased.

A phenol inhibition-coupled low temperature short-cut denitrification method belongs to the field of sewage treatment. The pre-denitrification operation mode of a sequencing batch reactor (SBR) is adopted, raw water and phenol compound are sufficiently utilized as carbon source, and additional carbon source is not added. When the temperature is linearly decreased to 15 DEG C from 25 DEG C and thephenol concentration is 0mg / L to 30mg / L, the system does not discharge sludge; when the phenol concentration is 60mg / L, the sludge concentration is kept at 4000mg / L; and when the phenol concentrationis 90mg / L, the sludge concentration is kept at 5000mg / L. The increase of the nitrite accumulation rate under low-concentration phenol is slow, and the nitrite accumulation rate is less than 5 percentwhen the phenol concentration is 0mg / L, reaches 22 percent at the final stage when the phenol concentration is 30mg / L, and is 43 percent at the final stage when the phenol concentration is 60mg / L; the affection of high-concentration phenol on the composition of system sludge is great, and the nitrite accumulation rate reaches 91 percent and is stably kept at more than 90 percent at the final stage. The method is applicable to industrial wastewater treatment in cold northern regions and can be adopted as a technical reference for the inhibition of the toxicity of phenolic compounds in urban sewage.