Method for producing marasmius androsaceus (L. ex Fr.) Fr. extracellular polysaccharide through submerged liquid state fermentation

A technology of Anluo Xiaopi umbrella and extracellular sugar, which is applied in the field of microorganisms and can solve the problems of seasonal restrictions, large investment, and difficulty in industrialization

Active Publication Date: 2014-10-01
JIANGSU SHENHUA PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical bottleneck of producing Anluo Xiaopi polysaccharide on an industrial scale, and adopt liquid fermentation to produce Anluo Xiaopi polysaccharide to overcome the disadvantages of long solid-state fermentation period, large investment, limited by seasons, and difficulty in industrialization. Adopting this invention can continuously, automatically and industrially produce high-quality Anluo Xiaopi polysaccharide

Method used

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  • Method for producing marasmius androsaceus (L. ex Fr.) Fr. extracellular polysaccharide through submerged liquid state fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The method for the production of the exopolysaccharide of Anluo Xiaopijiao by industrialized deep liquid fermentation of the present invention is carried out according to the following steps:

[0030] 1, the preparation of Anluo Xiaopi umbrella fermented liquid: the Anluo Xiaopi umbrella shake bottle bacterial strain that will activate is inoculated in seed culture medium (300L) by inoculum size 1%, and wherein seed culture medium is made up of glucose 3%, silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilation ratio is 1:1.0vvm, the tank pressure is 0.03Mpa, 26~28 Cultivate with shaking at ℃ for 5 days to get the first-class seed liquid of Anluo Xiaopi umbrella; the first-class seed liquid is inserted into the second-level seed medium (3000L) according to the inoculum size 15%, wherein the second-level seed medium consists of 3% glucos...

Embodiment 2

[0035] 1, the preparation of Anluo Xiaopi umbrella fermented liquid: inoculate the Anluo Xiaopi umbrella shake bottle bacterial strain that activates well by 1.5% of the inoculum in the seed culture medium (300L), wherein the seed culture medium consists of glucose 3%, silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilation ratio is 1:1.0vvm, the tank pressure is 0.03Mpa, 25~27 Cultivate with shaking at ℃ for 6 days to get the first-grade seed solution of Anluo Xiaopi umbrella; the first-grade seed solution is inserted into the second-grade seed medium (3000L) according to the inoculum size 15%, wherein the second-grade seed medium consists of 3% glucose, Silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilatio...

Embodiment 3

[0040] In order to better verify the superiority of the compound prescription of short-acting glucose and long-acting starch in the industrial production of Anluo Xiaopi polysaccharide in the present invention, several comparative experiments were done. Only adjusted the consumption of carbon source glucose or (and) starch in the test in the liquid fermentation medium, do not contain starch in the comparative example 1, and the glucose concentration is 1.5%; Do not contain glucose in the comparative example 2, the starch concentration is 2.5%, to In ratio 3, the glucose content is 2.5%, and the starch content is 0.5%; all the other conditions are the same as in Example 1, and the test results are shown in Table 1:

[0041] Table 1

[0042]

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Abstract

The invention belongs to the technical field of microbes, particularly relates to method for producing marasmius androsaceus (L. ex Fr.) Fr. extracellular polysaccharide through submerged liquid state fermentation, and in particular to a production method for marasmius androsaceus (L. ex Fr.) Fr. extracellular polysaccharide in an industrialization scale. The method comprises the following steps: conducting expanded matching on activated marasmius androsaceus (L. ex Fr.) Fr. strains through inoculating into a seeding tank, inoculating the liquid seeds subjected to expanded matching to a tailor-made liquid culture medium capable of producing polyose to conduct fermentation, separating the fermentation liquor, and extracting the extracellular polysaccharide. The invention aims to conduct a study on the deficiencies in the prior art, on the basis of continuously optimizing a fermentation culture medium (in particular to a carbon source), improving the biomass and the extracellular polysaccharide in a small-scale test and a pilot scale test, the problems such as parameter control in the fermentation process, extracting equipment lectotype, optimization and the like in the process from the small-scale test to the industrialized production are solved, finally, the marasmius androsaceus (L. ex Fr.) Fr. strains are grafted onto 10-20 t fermentation tanks to conduct industrialized production, and the marasmius androsaceus (L. ex Fr.) Fr. polyose with high quality is produced smoothly, so that the production with continuity, automatization, and industrialization is realized really and truly.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for producing exopolysaccharides from the umbrella of Anluo Xiaopi by submerged liquid fermentation. Background technique [0002] Anluo small umbrella, the scientific name is Marasmius androsaceus (L.ex Fr.) Fr. Alias ​​ghost hair needle, tea brown small umbrella, etc., belongs to Basidiomycetes, Agaricales (Agaricales), White Mushroom (ThichoLomataceae) , Small umbrella (Marasmius). Born in deep mountains and dense forests, cold and humid, acidic soil above dead branches, mainly distributed in Jilin, Hunan, Hubei, Guangdong and other provinces of China. The folks in our country have long used the rhizome cord of the wild Anluo Xiaopi umbrella to treat fracture pain, bruises, etc. According to the report of Guangdong Institute of Microbiology (1975), the Institute and Guangzhou No. 1 Pharmaceutical Factory obtained the fermentation culture of Anluo Xiaopi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 王文风徐国华徐玲王英燕张芙蓉杨亚威高岩
Owner JIANGSU SHENHUA PHARMA
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