A method for producing exopolysaccharide of Antrodia camphorata by submerged liquid fermentation

Abstract

The invention discloses a method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation, particularly an industrial-scale production method of Antrodia camphorata extracellular polysaccharides. The method comprises the following steps: inoculating activated Antrodia camphorata strain into a seed tank to perform amplification culture, inoculating the liquid seed into a purpose-made polysaccharide-producing liquid culture medium to perform fermentation, separating the fermentation liquid, and extracting the extracellular polysaccharides. In order to provide the yield of the extracellular polysaccharides, the method optimizes the formula of the fermentation culture medium, and adopts the compound formula against the carbon source part, thereby ensuring the biomass of the earlier-stage thallus and also ensuring the yield of the later-stage metabolites. The method can be used for preparing high-quality polysaccharides with different contents, and the polysaccharide content can be 20-50%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing antrodia camphorata exopolysaccharide by submerged liquid fermentation. Background technique [0002] Antrodia camphorata, also known as camphor fungus, Antrodia cinnamomea, Antrodia cinnamomea, Antrodia red, Camphor mushroom, Antrodia camphorata, Inner mushroom in Zhangku, Red camphor, belongs to Fungi, Basidiomycota, Laminaria, Polyporaceae , the genus of perennial fungi, is a rare and undeveloped edible and medicinal fungus endemic to Taiwan. It only grows on the inner wall of the rotten heartwood of the trunk of the unique camphor tree at an altitude of 450-2000 meters in the mountains of Taiwan, or on the dark and damp surface of the dead and fallen camphor wood. It is really not easy to find. Antrodia camphorata is a unique strain in Taiwan. In the natural environment, camphor tree is its only host, and general fungi cannot grow on...

AI Technical Summary

Problems solved by technology

There are also a small number of researchers from universities and colleges at home and abroad who have conducted research on the submerged fermentation of Antrodia camphorata, but their main focus is how to obtain higher biomass. Only a small number of researchers pay attention to the research on polysaccharides, the functional components of Antrodia camphorata. However, it is often limited to small-scale shake flask experiments, which belong to the basic research stage, and its biomass and active product extracellular sugar yields are relatively low, the production cost is too expensive, and its technology needs to be further improved. To achieve large-scale and The industrial production of Antrodia camphorata polysaccharide still needs a lot of research and experimental work

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