A method for producing exopolysaccharide of Antrodia camphorata by submerged liquid fermentation

A deep liquid fermentation and liquid fermentation technology, applied in the field of microorganisms, can solve the problems of high production cost and low yield of active product extracellular sugar, and achieve the effect of ensuring production

Active Publication Date: 2017-04-19
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also a small number of researchers from universities and colleges at home and abroad who have conducted research on the submerged fermentation of Antrodia camphorata, but their main focus is how to obtain higher biomass. Only a small number of researchers pay attention to the research on polysaccharides, the functional components of Antrodia camphorata. However, it is often limited to small-scale shake flask experiments, which belong to the basic research stage, and its biomass and active product extracellular sugar yields are relatively low, the production cost is too expensive, and its technology needs to be further improved. To achieve large-scale and The industrial production of Antrodia camphorata polysaccharide still needs a lot of research and experimental work

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  • A method for producing exopolysaccharide of Antrodia camphorata by submerged liquid fermentation

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Experimental program
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Effect test

Embodiment 1

[0030] The method for producing the exopolysaccharide of Antrodia camphorata by industrialized submerged liquid fermentation of the present invention is carried out according to the following steps:

[0031]1. Preparation of Antrodia camphorata fermentation broth: Inoculate the activated Antrodia camphorata shake flask strain into seed medium (300L) according to the inoculum size 1%, wherein the seed medium consists of 3% glucose, 0.2% peptone, and 1% yeast powder , Potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 4.8 with dilute sulfuric acid, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, shake culture at 26-28°C for 7 days Promptly obtain the Antrodia camphorata primary seed liquid; the primary seed liquid is inserted into the secondary seed medium (3000L) according to the inoculum size of 15%, wherein the secondary seed medium consists of 3% glucose, 0.2% peptone, and 1% yeast powder , potas...

Embodiment 2

[0036] 1. Preparation of Antrodia camphorata fermentation broth: inoculate the activated Antrodia camphorata shake flask strain into seed medium (300L) according to the inoculum size of 1.5%, wherein the seed medium consists of 3% glucose, 0.2% peptone, and 1% yeast powder , Potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 4.8 with dilute sulfuric acid, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, shake culture at 26-28°C for 8 days Promptly obtain the Antrodia camphorata primary seed liquid; the primary seed liquid is inserted into the secondary seed medium (3000L) according to the inoculum size of 15%, wherein the secondary seed medium consists of 3% glucose, 0.2% peptone, and 1% yeast powder , Potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 4.8 with dilute sulfuric acid, the ventilation ratio is 1:0.3vvm, the tank pressure is ...

Embodiment 3

[0041] In order to better verify the superiority of the compound prescription of short-acting glucose and long-acting starch in the industrial production of Antrodia camphorata polysaccharides in the present invention, several comparative experiments were done. In the test, only the consumption of carbon source glucose or (and) starch in the liquid fermentation medium was adjusted, and the comparative example 1 did not contain starch, and the glucose concentration was 2.0%; the comparative example 2 did not contain glucose, and the starch concentration was 2.5%. In ratio 3, the glucose content is 2.5%, and the starch content is 0.5%; all the other conditions are the same as Example 1 of the present invention, and the test results are shown in Table 1:

[0042] Table 1

[0043]

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Abstract

The invention discloses a method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation, particularly an industrial-scale production method of Antrodia camphorata extracellular polysaccharides. The method comprises the following steps: inoculating activated Antrodia camphorata strain into a seed tank to perform amplification culture, inoculating the liquid seed into a purpose-made polysaccharide-producing liquid culture medium to perform fermentation, separating the fermentation liquid, and extracting the extracellular polysaccharides. In order to provide the yield of the extracellular polysaccharides, the method optimizes the formula of the fermentation culture medium, and adopts the compound formula against the carbon source part, thereby ensuring the biomass of the earlier-stage thallus and also ensuring the yield of the later-stage metabolites. The method can be used for preparing high-quality polysaccharides with different contents, and the polysaccharide content can be 20-50%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing antrodia camphorata exopolysaccharide by submerged liquid fermentation. Background technique [0002] Antrodia camphorata, also known as camphor fungus, Antrodia cinnamomea, Antrodia cinnamomea, Antrodia red, Camphor mushroom, Antrodia camphorata, Inner mushroom in Zhangku, Red camphor, belongs to Fungi, Basidiomycota, Laminaria, Polyporaceae , the genus of perennial fungi, is a rare and undeveloped edible and medicinal fungus endemic to Taiwan. It only grows on the inner wall of the rotten heartwood of the trunk of the unique camphor tree at an altitude of 450-2000 meters in the mountains of Taiwan, or on the dark and damp surface of the dead and fallen camphor wood. It is really not easy to find. Antrodia camphorata is a unique strain in Taiwan. In the natural environment, camphor tree is its only host, and general fungi cannot grow on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 王文风徐国华徐玲王英燕张芙蓉杨亚威高岩
Owner JIANGSU SHENHUA PHARMA
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