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Liquid fermentation and purification method of antrodia camphorate exopolysaccharide

An extracellular polysaccharide, liquid fermentation technology, applied in the biological field, can solve the problems of separation and purification interference of Antrodia camphorata exopolysaccharide

Inactive Publication Date: 2018-06-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the incompletely utilized macromolecular substances in the medium after fermentation will also greatly interfere with the separation and purification of Antrodia camphorata exopolysaccharides in the later stage

Method used

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  • Liquid fermentation and purification method of antrodia camphorate exopolysaccharide
  • Liquid fermentation and purification method of antrodia camphorate exopolysaccharide
  • Liquid fermentation and purification method of antrodia camphorate exopolysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Liquid fermentation: The activated Antrodia cinnamomea strain is inoculated into a seed medium (120mL) according to the inoculum amount 15% (v / v). The composition of the seed medium is: glucose 50g / L, yeast extract 10g / L, MgSO 4 ·7H 2 O 1.0g / L, K 2 HPO 4 0.5g / L, citric acid 0.5g / L, natural pH. The filling volume is 120mL / 500mL Erlenmeyer flask, the seed solution of Antrodia cinnamomea is obtained by culturing at 28℃ for 3 days at a rotation speed of 130r / min; the seed solution is connected to the fermentation medium according to the 18% inoculum, and the composition of the fermentation medium is glucose 60g / L, yeast extract powder 16g / L, KH 2 PO 4 0.75g / L, MgSO 4 ·7H 2 O 0.77g / L, vitamin B 1 1g / L, adjust the initial pH to 5.0 with phosphate buffer. The filling volume is 120mL / 500mL, and the fermentation broth of Antrodia cinnamomea is obtained by culturing at 27°C for 10 days at a rotating speed of 100r / min.

[0024] 2. Separation of extracellular polysaccharides: Afte...

Embodiment 2

[0028] 1. Liquid fermentation: The activated Antrodia cinnamomea strain is inoculated into a seed medium (100mL) according to the inoculum amount 10%. The composition of the seed medium is: glucose 60g / L, yeast extract 12g / L, MgSO 4 ·7H 2 O 0.8g / L, K 2 HPO 4 1.0g / L, citric acid 0.3g / L, natural pH. The filling volume is 100mL / 500mL Erlenmeyer flask, cultured at 26℃ for 5 days at 140r / min to obtain Antrodia cinnamomea seed liquid; the seed liquid is connected to the fermentation medium according to the inoculum amount of 18%, and the composition of the fermentation medium is glucose 50g / L, yeast extract powder 20g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, vitamin B 1 0.5g / L, adjust the initial pH to 4.0 with phosphate buffer. The filling volume is 150mL / 500mL, and the fermentation broth of Antrodia cinnamomea is obtained by incubating at 25°C for 8 days at a speed of 150r / min.

[0029] 2. Separation of extracellular polysaccharides: After the fermentation, filter with quantitati...

Embodiment 3

[0033] On the basis of Example 1, adjust the content of each component in the fermentation medium

[0034] The composition of the fermentation medium is: glucose 40g / L, yeast extract 24g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O0.5g / L, vitamin B 1 1g / L, adjust the initial pH to 5.0 with phosphate buffer.

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Abstract

Belonging to the biotechnology field, the invention discloses a liquid fermentation and purification method of antrodia camphorate exopolysaccharide. The method includes: culturing an activated antrodia camphorate strain with a seed solution, then inoculating the antrodia camphorate strain into a fermentation medium that has no influence on later polysaccharide separation and purification for liquid fermentation, then separating the fermentation broth to obtain a crude antrodia camphorate exopolysaccharide component, and finally conducting ultrafiltration and column chromatography to obtain two exopolysaccharides with high content, wherein one exopolysaccharide is a polysaccharide component with a highly branched structure. The method provided by the invention has the characteristics of simple process and easy realization, not only greatly improves the yield of antrodia camphorate exopolysaccharide, but also avoids the interference of various additives in the medium on exopolysaccharide separation and purification.

Description

Technical field [0001] The invention relates to a method for liquid fermentation and purification of Antrodia camphorata extracellular polysaccharide, and belongs to the field of biotechnology. Background technique [0002] Antrodia camphorata (Antrodia camphorata, A. camphorate), also known as Antrodia camphorata, Antrodia camphorata, Antrodia camphorata, etc., belongs to the phylum Fungi, Basidiomycotina, Layer Fungi, Polyporaceae, and Pleuroporus. Rare medicinal and edible fungus. Antrodia cinnamomea mainly grows in dark, humid, and slightly lower temperature in the broad-leaved forest area of ​​Taiwan's East Coast Mountains at 450-2000m above sea level. It is suitable for a neutral and acidic environment. Antrodia cinnamomea is the only host in the natural environment of Antrodia cinnamomea. At present, the number is quite scarce and has been listed as a first-level conservation tree species. There are fewer cinnamomum cinnamomea trees that can grow Antrodia cinnamomea, resu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/645
CPCC08B37/0003C12P19/04
Inventor 陈磊王正齐张薄博许赣荣葛梦蝶
Owner JIANGNAN UNIV
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