A kind of method of submerged liquid state fermentation to produce exopolysaccharide of Anluo Xiaopijiao

A technology of Anluo Xiaopi umbrella and exopolysaccharide, which is applied in the field of microorganisms and can solve the problems of seasonal restrictions, large investment, and long solid-state fermentation cycle

Active Publication Date: 2016-08-24
JIANGSU SHENHUA PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the technical bottleneck of producing Anluo Xiaopi polysaccharide on an industrial scale, and adopt liquid fermentation to produce Anluo Xiaopi polysaccharide to overcome the disadvantages of long solid-state fermentation period, large investment, limited by seasons, and difficulty in industrialization. Adopting this invention can continuously, automatically and industrially produce high-quality Anluo Xiaopi polysaccharide

Method used

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  • A kind of method of submerged liquid state fermentation to produce exopolysaccharide of Anluo Xiaopijiao

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for the industrialized submerged liquid state fermentation of the present invention to produce the exopolysaccharide of Anluo Xiaopi is carried out according to the following steps:

[0030] 1, the preparation of Anluo Xiaopi umbrella fermented liquid: the Anluo Xiaopi umbrella shake bottle bacterial strain that will activate is inoculated in seed culture medium (300L) by inoculum size 1%, and wherein seed culture medium is made up of glucose 3%, silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilation ratio is 1:1.0vvm, the tank pressure is 0.03Mpa, 26~28 Cultivate with shaking at ℃ for 5 days to get the first-class seed liquid of Anluo Xiaopi umbrella; the first-class seed liquid is inserted into the second-level seed medium (3000L) according to the inoculum size 15%, wherein the second-level seed medium consists of 3% gluco...

Embodiment 2

[0035] 1, the preparation of Anluo Xiaopi umbrella fermented liquid: inoculate the Anluo Xiaopi umbrella shake bottle bacterial strain that activates well by 1.5% of the inoculum in the seed culture medium (300L), wherein the seed culture medium consists of glucose 3%, silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilation ratio is 1:1.0vvm, the tank pressure is 0.03Mpa, 25~27 Cultivate with shaking at ℃ for 6 days to get the first-grade seed solution of Anluo Xiaopi umbrella; the first-grade seed solution is inserted into the second-grade seed medium (3000L) according to the inoculum size 15%, wherein the second-grade seed medium consists of 3% glucose, Silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilatio...

Embodiment 3

[0040] In order to better verify the superiority of the compound prescription of short-acting glucose and long-acting starch in the industrial production of Anluo Xiaopi polysaccharide in the present invention, several comparative experiments were done. Only adjusted the consumption of carbon source glucose or (and) starch in the test in the liquid fermentation medium, do not contain starch in the comparative example 1, and the glucose concentration is 1.5%; Do not contain glucose in the comparative example 2, the starch concentration is 2.5%, to In ratio 3, the glucose content is 2.5%, and the starch content is 0.5%; all the other conditions are the same as in Example 1, and the test results are shown in Table 1:

[0041] Table 1

[0042]

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to a method for producing exopolysaccharides from the umbrella of Anluo Xiaopi by submerged liquid fermentation. Specifically, it is an industrial-scale production method of exopolysaccharides of Pygmy Anluo. The liquid seeds are inserted into a special polysaccharide-producing liquid medium for fermentation, and then the fermentation broth is separated to extract exopolysaccharides. The present invention studies the above-mentioned deficiencies in the prior art, on the basis of continuing to optimize the fermentation medium (especially the carbon source) and improving the biomass and exopolysaccharide in the small test and the pilot test, it solves the problem of scaling up from small test to industrial production The difficulties encountered in the fermentation process parameter control, extraction equipment selection, optimization, etc., were finally successfully grafted into a 10-20t fermenter for industrial production, and successfully produced high-quality Anluo Xiaopi polysaccharides, truly realizing the continuous , automated, industrialized production.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for producing exopolysaccharides from the umbrella of Anluo Xiaopi by submerged liquid fermentation. Background technique [0002] Anluo small umbrella, the scientific name is Marasmius androsaceus (L.ex Fr.) Fr. Alias ​​ghost hair needle, tea brown small umbrella, etc., belongs to Basidiomycetes, Agaricales (Agaricales), White Mushroom (ThichoLomataceae) , Small umbrella (Marasmius). Born in deep mountains and dense forests, cold and humid, acidic soil above dead branches, mainly distributed in Jilin, Hunan, Hubei, Guangdong and other provinces of China. The folks in our country have long used the rhizome cord of the wild Anluo Xiaopi umbrella to treat fracture pain, bruises, etc. According to the report of Guangdong Institute of Microbiology (1975), the Institute and Guangzhou No. 1 Pharmaceutical Factory obtained the fermentation culture of Anluo Xiaopi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 王文风徐国华徐玲王英燕张芙蓉杨亚威高岩
Owner JIANGSU SHENHUA PHARMA
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