The invention discloses a device and a method for synergistic inhibition of nitrifying bacteria activity to achieve partial nitrification employing free ammonia and free nitrous acid, and belongs to the field of biological nitrification of continuous flow process wastewater. The device mainly comprises a raw water tank, an intake pump, an intake pipe, an aerobic nitrification pond, a pH determinator, a pH sensor, a blast blower, an aerator tube, an air diffuser, a sludge reflux pipe, a sludge reflux pump, an aerobic nitrification pond drain pipe, a settling pond, a settling pond drain pipe, and a residue sludge discharge pipe. On the basis of the synergistic inhibition mechanism of the free ammonia (FA) and free nitrous acid (FNA) on activity of nitrite-oxidizing bacteria, rapid start and stable maintaining of partial nitrification at normal temperature are achieved by controlling the FA concentration inside the aerobic nitrification pond at 5.9-63.1mg/L, the problem of a bottleneck of partial nitrification of the continuous process is solved, and the device has the characteristics of being high in nitrification efficiency, rapid in reaction rate, and the like, the operation cost is saved, and excellent water is obtained.