Method for producing inonotus obliquus exopolysaccharides through submerged fermentation

A technology of Inonotus obliquus and extracellular sugar, applied in the field of microorganisms, can solve the problems of limitation, low yield of active product extracellular sugar, high production cost and the like

Active Publication Date: 2014-10-15
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also a small number of researchers from colleges and universities at home and abroad who have conducted research on the submerged fermentation of Inonotus obliquus, but often their main focus is how to obtain higher biomass, and only a small number of researchers pay attention to the functional components of Inonotus obliquus Research on polysaccharides, but the polysaccharides are mainly obtained through mycelium extraction, and only some graduate students from Zhejiang Sci-Tech University and Tianjin University of Science and Technology have carried out research on the production of exopolysaccharides from Inonotus o

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  • Method for producing inonotus obliquus exopolysaccharides through submerged fermentation

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Experimental program
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Embodiment 1

[0031] The method for producing Inonotus obliquus exopolysaccharide by industrialized deep liquid fermentation of the present invention is carried out according to the following steps:

[0032]1. Preparation of Inonotus obliquus fermented liquid: inoculate the shake flask bacterial strain of Inonotus obliquus well into seed culture medium (300L) by inoculum size 1%, wherein the seed culture medium consists of glucose 3%, silkworm chrysalis powder 0.3% , soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, and shake at 26-28°C Cultivate for 5 days to get the primary seed liquid of Inonotus obliquus; insert the primary seed liquid into the secondary seed medium (3000L) according to the inoculum size 15%, wherein the secondary seed medium consists of 3% glucose and 0.3% silkworm chrysalis powder %, soybean cake powder 1.8%, p...

Embodiment 2

[0037] 1. Preparation of Inonotus obliquus fermented liquid: inoculate the shake flask strain of Inonotus obliquus well into seed culture medium (300L) by inoculum size 1.5%, wherein the seed culture medium consists of glucose 3%, silkworm chrysalis powder 0.3% , soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, and shake at 25~27℃ Cultivate for 6 days to get the primary seed liquid of Inonotus obliquus; insert the primary seed liquid into the secondary seed medium (3000L) according to the inoculum size 15%, wherein the secondary seed medium consists of 3% glucose and 0.3% silkworm chrysalis powder %, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 7.0, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, 25~27℃ Culti...

Embodiment 3

[0042] In order to better verify the superiority of the compound prescription of short-acting glucose and long-acting starch in the industrial production of Inonotus obliquus polysaccharides in the present invention, several comparative experiments were done. Only adjusted the consumption of carbon source glucose or (and) starch in the test in the liquid fermentation medium, do not contain starch in the comparative example 1, and glucose concentration is 1.5%; Do not contain glucose in the comparative example 2, and the starch concentration is 2.5%, to In ratio 3, the glucose content is 2.5%, and the starch content is 0.5%; all the other conditions are the same as in Example 1, and the test results are shown in Table 1:

[0043] Table 1

[0044]

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Abstract

The invention belongs to the technical field of microorganisms, and relates to a method for producing inonotus obliquus exopolysaccharides through submerged fermentation, particularly to a method for industrial-scale production of the inonotus obliquus exopolysaccharides. The method comprises the steps as follows: activated inonotus obliquus strains are inoculated into a seed tank for propagation, then propagated liquid seeds are inoculated into a specially-made polysaccharide-producing liquid culture medium for fermentation, and a fermentation liquid is separated to extract the exopolysaccharides. According to the method, research is conducted to overcome defects in the prior art, on the premises that a fermentation culture medium (especially a carbon source) is continuously optimized through small and medium tests and the biomass and the exopolysaccharides are increased, the problems of fermentation process parameter control, extracting equipment model selection, optimization and the like in the process of enlargement from the small tests to industrial production are solved, the strains are successfully inoculated into fermentation tanks of 10-20 t finally for industrial production, the high-quality inonotus obliquus polysaccharides are produced smoothly, and the continuous, automatic and industrial production is actually realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing exopolysaccharide of Inonotus obliquus by submerged liquid fermentation. Background technique [0002] Inonotus obliquus, also known as Chaga, belongs to Basidiomycotina, Laminomycetes, Nonbryomycetes, Polyporaceae, and Poriophaeris genus. It is a large parasitic medicinal fungus born on birch trees. Its fruiting body presents a black block shape similar to carbon, and is a rare medicinal fungus mainly distributed in northern North America, Russia, Finland, Heilongjiang of China, Hokkaido of Japan and other regions [0003] Inonotus obliquus mainly parasitizes under the bark of other birch species such as white birch, elm, and silver birch. The fruiting body, that is, the surface of the sclerotium is black and deeply cracked, and the front part of the fungal tube is cracked. Egg-shaped, it is a wood-rot fungus that lives in cold regions....

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 徐玲徐国华王文风王英燕张芙蓉杨亚威高岩
Owner JIANGSU SHENHUA PHARMA
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