Method for producing inonotus obliquus exopolysaccharides through submerged fermentation

Abstract

The invention belongs to the technical field of microorganisms, and relates to a method for producing inonotus obliquus exopolysaccharides through submerged fermentation, particularly to a method for industrial-scale production of the inonotus obliquus exopolysaccharides. The method comprises the steps as follows: activated inonotus obliquus strains are inoculated into a seed tank for propagation, then propagated liquid seeds are inoculated into a specially-made polysaccharide-producing liquid culture medium for fermentation, and a fermentation liquid is separated to extract the exopolysaccharides. According to the method, research is conducted to overcome defects in the prior art, on the premises that a fermentation culture medium (especially a carbon source) is continuously optimized through small and medium tests and the biomass and the exopolysaccharides are increased, the problems of fermentation process parameter control, extracting equipment model selection, optimization and the like in the process of enlargement from the small tests to industrial production are solved, the strains are successfully inoculated into fermentation tanks of 10-20 t finally for industrial production, the high-quality inonotus obliquus polysaccharides are produced smoothly, and the continuous, automatic and industrial production is actually realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing exopolysaccharide of Inonotus obliquus by submerged liquid fermentation. Background technique [0002] Inonotus obliquus, also known as Chaga, belongs to Basidiomycotina, Laminomycetes, Nonbryomycetes, Polyporaceae, and Poriophaeris genus. It is a large parasitic medicinal fungus born on birch trees. Its fruiting body presents a black block shape similar to carbon, and is a rare medicinal fungus mainly distributed in northern North America, Russia, Finland, Heilongjiang of China, Hokkaido of Japan and other regions [0003] Inonotus obliquus mainly parasitizes under the bark of other birch species such as white birch, elm, and silver birch. The fruiting body, that is, the surface of the sclerotium is black and deeply cracked, and the front part of the fungal tube is cracked. Egg-shaped, it is a wood-rot fungus that lives in cold regions....

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Problems solved by technology

There are also a small number of researchers from colleges and universities at home and abroad who have conducted research on the submerged fermentation of Inonotus obliquus, but often their main focus is how to obtain higher biomass, and only a small number of researchers pay attention to the functional components of Inonotus obliquus Research on polysaccharides, but the polysaccharides are mainly obtained through mycelium extraction, and only some graduate students from Zhejiang Sci-Tech University and Tianjin University of Science and Technology have carried out research on the production of exopolysaccharides from Inonotus obliquus by liquid fermentation, but the main shortcomings are: ① They are all limited to small-scale shake flask experiments, which belong to the basic research stage; ② It can be seen from their data that the culture period is about 9 days, the biomass is 1.0-1.1g / 100ml, and the exopolysaccharide production is 0.11-0.14 g / 100ml, the yield of biomass and active product extracellular sugar is too low, the production cost is too expensive, and the process needs to be further improved. To achieve large-scale and industrial production of Inonotus obliquus polysaccharides, a lot of research and research are needed. experimental work

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