Method for producing xylaria nigripes exopolysaccharides through submerged fermentation

Abstract

The invention discloses a method for producing xylaria nigripes exopolysaccharides through submerged fermentation. The method comprises the steps as follows: activated xylaria nigripes strains are inoculated into a seed tank for propagation, then propagated liquid seeds are inoculated into a specially-made polysaccharide-producing liquid culture medium for fermentation, and a fermentation liquid is separated to extract the exopolysaccharides. According to the method, research is conducted to solve the technical problems encountered during amplification from small tests to industrial production, on the premises that a fermentation culture medium (especially a carbon source) is continuously optimized through small and medium tests and the biomass and the exopolysaccharides are increased, the problems of fermentation process parameter control, extracting equipment model selection, optimization and the like during enlargement are solved, the strains are successfully inoculated into fermentation tanks of 10-20 t finally for industrial production, the high-quality xylaria nigripes exopolysaccharides are produced smoothly, and the continuous, automatic and industrial production is actually realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing Wulingshen exopolysaccharide by submerged liquid fermentation. Background technique [0002] Wulingshen belongs to the class Ascomycetes, and is a traditional medicinal fungus. It grows on the abandoned nests of black-winged earth-dwelling termites deep underground. It is regarded as a rare and valuable medicinal material by the research and application fields of medicinal fungi in the world, and has extensive medicinal value. [0003] Wuling ginseng grows on sunny and warm hill slopes or termite waste fungi on river embankment soil slopes. It is densely covered with mycelia of C. nigriscens on the fungus garden. In the later stage of its growth, part of the sclerotium is densely formed. , commonly known as "Wuling Ginseng", weighing about 5-30 grams, spherical, spherical or irregular flat strips, 2-12 cm in diameter, brown or black in a...

AI Technical Summary

Problems solved by technology

At present, there are only a few colleges and universities in China to carry out research on the production of Wulingshen by deep liquid fermentation, and most of them are limited to the level of small experiments. They are all researched with the main goal of obtaining the mycelium of Wulingshen. There are almost no relevant reports on the production of functional component polysaccharides of Wuling ginseng by liquid fermentation, and there is no research to solve technical problems such as the selection of medium formula, control of fermentation operating parameters, and research on polysaccharide extraction conditions encountered in the process of enlarging from small-scale grafting to industrial production. , so at present there is no real industrialized liquid fermentation production of Wuling ginseng polysaccharide in China

Images