Cultivation method for preventing morchella strains from forming mycelium pellets

Abstract

The invention belongs to the technical field of biology, and particularly relates to a cultivation method for preventing morchella strains from forming mycelium pellets. The method comprises the following steps: (1) preparing morchella mother liquor; (2) preparing a morchella solid culture medium; (3) preparing a morchella seed solution; and (4) preparing morchella liquid strains; The cultivation method scientifically and reasonably overcomes the defect of contradiction between mycelium balling and biomass in actual production, in the prior art, due to incompatibility of a production process, the mycelium balling aspect and the biomass aspect are not matched, and by means of the cultivation method, mycelium balling is avoided, biomass is improved to the maximum extent, the problems of non-uniform dissolved oxygen and blockage are avoided, and the urgent demand of the market on the morchella is met to the greatest extent.

Description

technical field [0001] The application belongs to the field of biotechnology, and in particular relates to a cultivation method for preventing morel strains from forming mycelium balls. Background technique [0002] Morchella is a rare species of edible fungus. It is not only a treasure on the banquet, but also a well-known food tonic product. There is a folk saying that "eat morel every year, and walk all over the mountain at 80". Morchella is flat in nature and sweet in taste. It has the functions of benefiting the intestines and stomach, helping digestion, reducing phlegm and regulating qi, nourishing the kidney, strengthening yang, nourishing the brain, and refreshing. It has a very high market demand. In the process of Morchella Morchellaspp. artificial field cultivation, the traditional method is to cultivate solid strains, but the original species of this preparation method is time-consuming, high in cost, low in purity, and inconsistent in age. This kind of solid se...

AI Technical Summary

Problems solved by technology

Aiming at the problems such as the low growth vigor of the hickory chick liquid spawn mycelia prepared by the existing method, the large diameter of the mycelium, and the blockage of equipment, the application provides a preparation method of the hickory chick liquid spawn, comprising the following steps: a 1. Inoculate the mother species of hickory chick in the PDA solid medium, and cultivate it at a constant temperature of 22~25°C for 5~10 days; b. Get the solid medium and inoculate it into a large square baffle shaker flask equipped with liquid medium , cultured on a shaker for 2 to 4 days; c. Add liquid culture medium to the liquid strain fermenter, after sterilization and cooling, inoculate the liquid strain of Morchella in a shaker bottle, under sterile conditions, at 24 to 28°C Cultivate for 1 to 3 days to obtain morel liquid strains. The unique four-sided large baffle shaker flask is used to change the fluid force field in the ordinary shaker flask, intensify the liquid stirring, increase the dissolved oxygen in the medium, and promote the new hyphae. Germination of mycelium, inhibition of mycelium balling, the cultured shake flask mycelium is flocculent, no balling, the vitality of mycelium balls is better, and it is not easy to block equipment. Using the method of this application, the final mycelium forming balls The diameter is 0.5-1.2mm, and the diameter of mycelium is small; the density of mycelium balls reaches about 2967 / mL, and the dry weight is about 16mg / mL. The optimal level of bacteria liquid culture

[0006] The method described in the above patent can indeed solve the problem that the mycelium aggregates into mycelial balls to solve the problem of clogging, but it faces the problem that the biomass and mycelial activity cannot reach the same level of liquid strain cultivation, which reduces the output and is difficult to meet the market demand. ;

[0007] The greater the biomass of the morel mycelium collected, the more vigorous the growth of the morel mycelium under this condition, and the more favorable the growth of the morel mycelium under this condition. During the liquid culture process of mycelia, the mycelium is very easy to entangle and form a round or oval mycelium ball with a tight texture. If the mycelium ball is too large, it will easily cause the pipeline of the fermentation and inoculation equipment to be blocked. Therefore, how to Solving mycelium agglomeration into mycelial balls is the primary problem of liquid strain cultivation without reducing mycelial biomass and maintaining high activity;

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