A method for producing exopolysaccharide of Inonotus obliquus by submerged liquid fermentation

Abstract

The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing exopolysaccharide of Inonotus obliquus by submerged liquid fermentation. Specifically, it is an industrial-scale production method of Inonotus obliquus exopolysaccharide. The method is to expand the activated Inonotus obliquus strains by inserting them into the seed tank, and then add the expanded liquid The seeds are inserted into a special polysaccharide-producing liquid medium for fermentation, and then the fermentation broth is separated to extract exopolysaccharides. The present invention studies the above-mentioned deficiencies in the prior art, on the basis of continuing to optimize the fermentation medium (especially the carbon source) and improving the biomass and exopolysaccharide in the small test and the pilot test, it solves the problem of scaling up from small test to industrialized production The difficulties encountered in the fermentation process parameter control, extraction equipment selection, optimization, etc., were finally successfully grafted into a 10-20t fermenter for industrial production, and successfully produced high-quality Inonotus obliquus polysaccharides, truly realizing continuous, Automated, industrialized production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing exopolysaccharide of Inonotus obliquus by submerged liquid fermentation. Background technique [0002] Inonotus obliquus, also known as Chaga, belongs to Basidiomycotina, Laminomycetes, Nonbryomycetes, Polyporaceae, and Poriophaeris genus. It is a large parasitic medicinal fungus born on birch trees. Its fruiting body presents a black block shape similar to carbon, and is a rare medicinal fungus mainly distributed in northern North America, Russia, Finland, Heilongjiang of China, Hokkaido of Japan and other regions [0003] Inonotus obliquus mainly parasitizes under the bark of other birch species such as white birch, elm, and silver birch. The fruiting body, that is, the surface of the sclerotium is black and deeply cracked, and the front part of the fungal tube is cracked. Egg-shaped, it is a wood-rot fungus that lives in cold regions....

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Problems solved by technology

There are also a small number of researchers from colleges and universities at home and abroad who have conducted research on the submerged fermentation of Inonotus obliquus, but often their main focus is how to obtain higher biomass, and only a small number of researchers pay attention to the functional components of Inonotus obliquus Research on polysaccharides, but the polysaccharides are mainly obtained through mycelium extraction, and only some graduate students from Zhejiang Sci-Tech University and Tianjin University of Science and Technology have carried out research on the production of exopolysaccharides from Inonotus obliquus by liquid fermentation, but the main shortcomings are: ① They are all limited to small-scale shake flask experiments, which belong to the basic research stage; ② It can be seen from their data that the culture period is about 9 days, the biomass is 1.0-1.1g / 100ml, and the exopolysaccharide production is 0.11-0.14 g / 100ml, the yield of biomass and active product extracellular sugar is too low, the production cost is too expensive, and the process needs to be further improved. To achieve large-scale and industrial production of Inonotus obliquus polysaccharides, a lot of research and research are needed. experimental work

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