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Method for promoting accumulation of heterotrophic microalgae oil through combination of melatonin and nitrogen deficiency

A technology of microalgae oil and melatonin, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low oil content in algae cells, poor quality of algae cells, unsuitable for accumulating oil, etc., to achieve The molecular weight is small, the process is simple and convenient, and the effect of improving the accumulation of oil

Inactive Publication Date: 2018-09-28
KUNMING UNIV OF SCI & TECH
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Problems solved by technology

[0003] Under heterotrophic conditions, microalgae can quickly accumulate biomass by using organic carbon sources such as glucose, which greatly shortens the growth cycle of algal cells and improves the yield of cell biomass. However, the quality of algal cells cultured under heterotrophic conditions is poor, and generally obtained The algal cell oil content is low, so the heterotrophic stage is generally not suitable for accumulating oil

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  • Method for promoting accumulation of heterotrophic microalgae oil through combination of melatonin and nitrogen deficiency

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Effect test

Embodiment 1

[0025] Embodiment 1: A kind of method that utilizes melatonin combined with nitrogen deficiency to promote the accumulation of oil in heterotrophic microalgae, the specific steps are as follows:

[0026] (1) Preparation of induced algae liquid: At a temperature of 25°C, the BG-11 basal medium with 10 g / L glucose as the carbon source was used for heterotrophic culture of Monosticema, and the growth of Monostipola was in the logarithmic growth phase. Collect the algal cells in the later stage, dilute and resuspend the algal cells to 0.85 g / L with nitrogen-deficient BG-11 medium as the induced algal liquid;

[0027] (2) Induce algae cells to accumulate oil: prepare 100 μmol / L melatonin mother solution with absolute ethanol, add melatonin mother solution to the induced algae solution in step (1) to dilute melatonin concentration to 1 μmol / L , and placed at a temperature of 25°C and a light intensity of 30 μmol m −2 the s −1 Under the conditions, the cold light lamp is continuou...

Embodiment 2

[0029] Embodiment 2: A kind of method that utilizes melatonin combined with nitrogen deficiency to promote the accumulation of oil in heterotrophic microalgae, the specific steps are as follows:

[0030] (1) Preparation of induced algae liquid: At a temperature of 24°C, the BG-11 basal medium with 10 g / L glucose as the carbon source was used for heterotrophic culture of Monosticema, and the growth of Monostipola was in the logarithmic growth phase Collect the algal cells in the later stage, dilute and resuspend the algal cells to 0.85 g / L with nitrogen-deficient BG-11 medium as the induced algal liquid;

[0031] (2) Induce algae cells to accumulate oil: prepare 100 μmol / L melatonin mother solution with absolute ethanol, add melatonin mother solution to the induced algae solution in step (1) to dilute melatonin concentration to 0.1 μmol / L , and placed at a temperature of 24°C and a light intensity of 29 μmol m −2 the s −1 Under the conditions, the cold light lamp is continuous...

Embodiment 3

[0033] Embodiment 3: Embodiment 3: a kind of method utilizing melatonin combined with nitrogen deficiency to promote the accumulation of oil in heterotrophic microalgae, the specific steps are as follows:

[0034] (1) Preparation of induced algae liquid: At a temperature of 25°C, the BG-11 basal medium with 10 g / L glucose as the carbon source was used for heterotrophic culture of Monosticema, and the growth of Monostipola was in the logarithmic growth phase. Collect the algal cells in the later stage, dilute and resuspend the algal cells to 1 g / L with nitrogen-deficient BG-11 medium as the induced algal liquid;

[0035] (2) Induce algae cells to accumulate oil: prepare 100 μmol / L melatonin mother solution with absolute ethanol, add melatonin mother solution to the induced algae solution in step (1) to dilute melatonin concentration to 2 μmol / L , and placed at a temperature of 26°C and a light intensity of 31 μmol m −2 the s −1 Under the conditions, the cold light lamp is co...

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Abstract

The invention relates to a method for promoting accumulation of heterotrophic microalgae oil through combination of melatonin and nitrogen deficiency, and belongs to the field of microalgae bio-technology. The method comprises the following steps: implementing heterotrophic culture of monoraphidium with the participation of a BG-11 basal culture medium which takes 10g / L glucose as a carbon sourceat a temperature of 24-26 DEG C, collecting algae cells in the later period of logarithmic phase of the monoraphidium, and diluting and re-suspending the algae cells via a BG-11 culture medium with nitrogen deficiency so as to obtain 0.85-1g / L inducing algae liquid; preparing 100[mu]mol / L melatonin mother liquid via absolute ethyl alcohol, adding the melatonin mother liquid to the inducing algae liquid so that melatonin concentration is diluted to 0.1-10[mu]mol / L, and implementing inducing culture at a temperature of 24-26 DEG C and under a light condition; and extracting oil in the algae cells via an organic solvent. The method provided by the invention is simple to operate, an algae cell inducing cycle can be shortened, an oil content can be improved and microalgae growth can be guaranteed; and such problems as biomass dropping, low oil content and the like in a microalgae industrial inducing process can be solved.

Description

technical field [0001] The invention relates to a method for promoting oil accumulation of heterotrophic microalgae by using melatonin combined with nitrogen deficiency, and belongs to the field of microalgae biotechnology. Background technique [0002] Microalgae biomass is used as a raw material to replace terrestrial plants to produce biofuels, because it can perform photosynthesis and can effectively use solar energy to convert water, CO 2 and inorganic salts into organic matter. Microalgae have the advantages of high photosynthetic utilization rate, fast growth rate, low requirements for growth environment, and high content of energy storage substances (oil, hydrocarbon) and so on, which have been widely studied (Mata T M, Martins AA, Caetano N S. Microalgae for biodiesel production and other applications: areview[J]. Renewable and sustainable energy reviews, 2010, 14(1): 217-232.). [0003] Under heterotrophic conditions, microalgae can quickly accumulate biomass by ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/12C12R1/89
CPCC12N1/12C12P7/6463
Inventor 余旭亚赵永腾李大菲
Owner KUNMING UNIV OF SCI & TECH
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