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A kind of silk fibroin/octacalcium phosphate composite bone scaffold material and its preparation method

A technology of silk fibroin and octacalcium phosphate, applied in the field of materials, to achieve good degradation performance and mechanical properties, increase strength, and enhance osteogenic performance

Active Publication Date: 2018-05-15
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, there is no porous scaffold prepared by silk fibroin / octacalcium phosphate complex, and the present invention comes from this

Method used

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  • A kind of silk fibroin/octacalcium phosphate composite bone scaffold material and its preparation method
  • A kind of silk fibroin/octacalcium phosphate composite bone scaffold material and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1. Dissolve silk fibroin in 50 mL of 40 mM calcium nitrate solution to prepare a solution with a silk fibroin concentration of 0.1% by weight.

[0029] 2. Prepare 50 mL of a sodium dihydrogen phosphate solution with a concentration of 40 mM.

[0030] 3. Under 68°C and stirring conditions, slowly add the silk fibroin-containing calcium nitrate solution prepared in step 1) to the sodium dihydrogen phosphate solution prepared in step 2), and continue to stir for 1 hour to finally obtain a white precipitate Things. The white precipitate was washed three times by centrifugation, placed at -80°C for 1 day, and freeze-dried for 2 days to obtain a silk fibroin / octacalcium phosphate complex. The infrared spectrum is as follows figure 1 Shown

[0031] 4. Grind the silk fibroin / octacalcium phosphate composite obtained in step 3) into a powder with a mortar, weigh out 20 mg of the powder, then mix it with 1 mL of silk fibroin solution with a concentration of 6 wt%, and pour it into a cyl...

Embodiment 2

[0034] 1. Dissolve silk fibroin in 50 mL of 80 mM calcium nitrate solution to prepare a solution with a silk fibroin concentration of 0.5 wt%.

[0035] 2. Prepare 50 mL of a sodium dihydrogen phosphate solution with a concentration of 80 mM.

[0036] 3. Under stirring conditions at 70°C, slowly add the silk fibroin-containing calcium nitrate solution prepared in step 1) to the sodium dihydrogen phosphate solution prepared in step 2), and continue to stir for 1.5 hours to obtain a white precipitate. Things. After centrifugal washing the white precipitate for three times, place it at -80°C for 1 day and freeze-dry for 2 days to obtain a silk fibroin / octacalcium phosphate complex;

[0037] 4. Grind the silk fibroin / octacalcium phosphate composite obtained in step 3) into a powder with a mortar, weigh out 50 mg of the powder, then mix it with 1 mL of silk fibroin solution with a concentration of 10 wt%, and pour it into a cylindrical mold. Placed at -80°C for 1 day and freeze-dried for...

Embodiment 3

[0040] 1. Dissolve silk fibroin in 120 mL of 40 mM calcium nitrate solution to prepare a solution with a silk fibroin concentration of 2 wt%.

[0041] 2. Prepare 120 mL of sodium dihydrogen phosphate solution with a concentration of 40 mM.

[0042] 3. Under the condition of heating and stirring at 68°C, slowly add the silk fibroin-containing calcium nitrate solution prepared in step 1) to the sodium dihydrogen phosphate solution prepared in step 2), and continue to stir for 1.5 hours to obtain a white precipitate. Things. After centrifugal washing the white precipitate for three times, place it at -80°C for 1 day and freeze-dry for 2 days to obtain a silk fibroin / octacalcium phosphate complex;

[0043] 4. Grind the silk fibroin / octacalcium phosphate composite obtained in step 3) into a powder with a mortar, weigh 100 mg of the powder, and then mix it with 1 mL of silk fibroin solution with a concentration of 10 wt%, and pour it into a cylindrical mold. Placed at -80°C for 1 day and...

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Abstract

The invention relates to the technical field of materials, and discloses a silk fibroin / octacalcium phosphate composite bone scaffold material. The silk fibroin / octacalcium phosphate composite bone scaffold material is prepared from silk fibroin and octacalcium phosphate, wherein the mass ratio of silk fibroin to octacalcium phosphate is 10:1-10. A preparation method includes the steps that a sodium dihydrogen phosphate solution and a calcium nitrate solution containing silk fibroin are prepared, wherein the concentration of the sodium dihydrogen phosphate solution is equal to that of the calcium nitrate solution; the two solutions are mixed with heating and stirring to obtain white precipitate, and a fibroin / octacalcium phosphate composite is obtained after centrifugal washing and freeze-drying; the fibroin / octacalcium phosphate composite and a high-concentration silk fibroin solution are evenly mixed, the mixture is injected into a mould, and a porous scaffold is obtained after freeze-drying; the silk fibroin / octacalcium phosphate composite bone scaffold material is obtained after aftertreatment. The prepared silk fibroin / octacalcium phosphate composite bone scaffold material has good biocompatibility, degradability, mechanical performance and osteogenesis performance, is easy to prepare and can be applied to the field of bone tissue engineering.

Description

Technical field [0001] The invention relates to the technical field of materials, in particular to a silk fibroin / octacalcium phosphate composite bone scaffold material and a preparation method. Background technique [0002] Bone tissue engineering technology relies on biological materials to induce bone tissue regeneration and in situ bone repair from the perspective of cells, scaffolds and active factors. However, the current repair effect of bone tissue engineering scaffold materials is still different from that of autologous bone. From the perspective of bionics, preparing artificial bone graft materials that mimic the composition and structure of natural bone is an important research content of bone repair materials. Phosphate ceramic materials can form a strong bone bond with bone, and have good biocompatibility and biological activity. It is an ideal substitute material for bone tissue in clinic. Hydroxyapatite is the most abundant inorganic component in bone, but it is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/46A61L27/22A61L27/50
CPCA61L27/365A61L27/3691A61L27/46A61L27/50A61L27/56A61L2400/18A61L2430/02C08L89/00
Inventor 韩凤选李斌龚嘉伟过倩萍朱彩虹杨惠林
Owner SUZHOU UNIV
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