Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing lipid through mixed culture of microalgae

A mixed culture and microalgae technology, which is applied in the field of biotechnology and bioenergy, can solve the problems of low efficiency, achieve high carbon sequestration efficiency, alleviate the greenhouse effect and exhaust pollution problems, and achieve the effects of high biomass and oil content

Active Publication Date: 2016-06-29
CHINA PETROLEUM & CHEM CORP +1
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For existing algal species that cannot tolerate and absorb high concentrations of CO 2 , fixed CO 2 In order to solve the problem of low efficiency, the present invention provides a 2 And the method for producing oil by mixed culture of microalgae under alkaline environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing lipid through mixed culture of microalgae
  • Method for producing lipid through mixed culture of microalgae
  • Method for producing lipid through mixed culture of microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation of embodiment 1 microalgae seed liquid

[0018] BG11 medium was used for microalgae culture, and the formulation of the medium was shown in Table 1 and Table 2.

[0019] Table 1BG11 medium

[0020]

[0021] * Composition of A5+Cosolution in Table 2 and Table 1

[0022]

[0023] First prepare the BG11 liquid medium according to Table 1 and Table 2, adjust the pH of the medium for cultivating Scenedesmus obliquus FSH-Y2 to 10, adjust the pH of the medium for cultivating Monostipella SS-B1 to 7.5, and then Scenedesmus obliquus FSH-Y2 and Single needle sp. SS-B1 were inoculated in the above medium respectively. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture to the logarithmic growth phase, and Scenedesmus obliquus FSH-Y2 is obtained Seed solution and Monostipella SS-B1 seed solution, the above seed so...

Embodiment 2

[0024] The preparation of embodiment 2 microalgae oil

[0025] (1) In the photobioreactor, add the FSH-Y2 seed solution prepared in Example 1 and the microalgae medium, the volume ratio of the FSH-Y2 seed solution to the medium is 1:10, and the medium is BG11 medium, The pH value of the culture medium was controlled at 10, the light intensity of the culture was 5000Lux, the light cycle was 24h, the light-dark time ratio was 14:10, and a mixed gas of nitrogen and carbon dioxide was introduced, and the carbon dioxide content was 5v%.

[0026](2) After culturing for 2 days, insert the seed solution of Monospina SS-B1 prepared in Example 1, the volume ratio of the seed solution to the medium is 1:10, the pH of the medium is controlled at 8, and nitrogen and carbon dioxide are introduced into the solution. Mixed gas, where CO 2 The content is 30v%.

[0027] (3) After cultivating for 7 days, enter the stable period, end the culturing, harvest the microalgae cells by centrifugation...

Embodiment 3

[0028] The preparation of embodiment 3 microalgae oil

[0029] (1) In the photobioreactor, add the FSH-Y2 seed solution prepared in Example 1 and the microalgae medium, the volume ratio of the FSH-Y2 seed solution to the medium is 1:5, and the medium is BG11 medium, The pH value of the culture medium was controlled at 12, the light intensity of the culture was 5000Lux, the light cycle was 24h, the light-dark time ratio was 14:10, and a mixed gas of nitrogen and carbon dioxide was introduced, and the carbon dioxide content was 5v%.

[0030] (2) After culturing for 3 days, insert the seed solution of Monospina SS-B1 prepared in Example 1, the volume ratio of the seed solution to the medium is 1:10, the pH of the medium is controlled at 10, and nitrogen and carbon dioxide are introduced into the solution. Mixed gas, where CO 2 The content is 25v%.

[0031] (3) After cultivating for 7 days, enter the stable period, end the culturing, harvest the microalgae cells by centrifugatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for producing lipid through mixed culture of microalgae. The method comprises the following steps: (1) adding a microalgae culture medium and scenedesmus obliqnus FSH-Y2 seed solution into a photo-biological reactor, and culturing for 2-5 days, wherein the pH value of a culture system is regulated to be 10-12, and the content of CO2 in the introduced gas is controlled to be 5v% or below; (2) adjusting the pH of the culture system to be 8-10, and inoculating with monoraphidium sp SS-B1 seed solution for mixed culture, wherein the content of CO2 in the introduced gas is 5v%-45v%; and (3) culturing till the stationary growth stage is finished, and harvesting microalgae cells, wherein scenedesmus obliqnus FSH-Y2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 11, 2012, and has the preservation number CGMCC No.6551, and monoraphidium sp SS-B1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 15, 2013, and has the preservation number CGMCC No.7479. With the adoption of the method, the tolerance and solubility of the microalgae culture system to high-concentration CO2 are improved, the carbon sequestration efficiency is improved, the harvest yield of the microalgae lipid is obviously improved, and the production of biodiesel is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology and bioenergy, in particular to a 2 And the method for producing oil by mixed culture of microalgae under alkaline environment. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalg...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64B01D53/84B01D53/62C12R1/89
CPCY02A50/20Y02E50/10Y02P20/59
Inventor 师文静廖莎姚新武樊亚超孙启梅王领民高大成李晓姝
Owner CHINA PETROLEUM & CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com