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Acquisition and application of grease-producing monoraphidium LB50

A technology of monopods and green algae, which is applied in the field of screening and cultivation of oleaginous microalgae, can solve problems such as the shortage of good algae strains, and achieve the effects of reducing costs and preventing damage

Active Publication Date: 2014-02-05
INST OF AQUATIC LIFE ACAD SINICA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of insufficient fine algae strains used to produce microalgae oil in the prior art, and to provide the acquisition and application of Monospina oleaginum LB50, that is, to collect water samples in the wild waters of Inner Mongolia, separate and purify Obtained an oleaginous microalgae with stress resistance and easy open culture, and established methods for cultivating and collecting

Method used

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  • Acquisition and application of grease-producing monoraphidium LB50
  • Acquisition and application of grease-producing monoraphidium LB50
  • Acquisition and application of grease-producing monoraphidium LB50

Examples

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example 1

[0038] Example 1. Semi-continuous cultivation in a circular photoreactor

[0039] Prepare the culture medium according to the medium formula described above, at 0.1 m 2 Inoculate and culture in a circular photoreactor with a culture medium volume of 20 L. Initial OD 680 The value is 0.05, the temperature is controlled at 25-30°C, and the light intensity is 150 μE / m 2 s continuous light, pass CO2 into the culture medium for 1 hour every day 2 , sampling to measure OD 680 value. OD 680 When the value reaches 6.0, collect 1 / 2 of the algae liquid and supplement 1 / 2 of the fresh medium for semi-continuous culture, and then collect the algae liquid every 5 days and feed it once. Adjust the pH of the collection container to 6 with dilute hydrochloric acid, add chitosan to 7.5 mg / L, and the flocculation and sedimentation efficiency reaches more than 90% in 10 minutes. Remove the supernatant from the flocculated algae liquid to obtain concentrated algae cells. The algae cells w...

example 2

[0042] Example 2. Outdoor track pool cultivation

[0043] According to the medium formula of the algae strain, the local groundwater in Inner Mongolia was used to prepare the culture medium. 2 Runway pools for open culture (see Figure 4 ), the volume of culture medium was 750 L. The flow rate of the runway pool is controlled to 0.04 m / s by a wheel-shaped agitator; the water temperature is about 30°C during the day and about 24°C at night; the light intensity varies with the weather, and the average light intensity is 600μE / m 2s, in a dark state at night; sample and measure OD every day 680 value, cultured to OD 680 When the value reaches 1.5, start to collect 1 / 5 of the volume of algae fluid, then add 1 / 5 of the fresh culture fluid for semi-continuous culture, and then collect feed once every 3 days. The collected algae liquid is observed under a microscope and maintains more than 99% pure species, with little contamination by bacteria and other algae species. As in Exam...

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Abstract

The invention discloses acquisition and application of grease-producing monoraphidium LB50, and relates to screening and culture utilization technologies of the grease-producing monoraphidium. An algae species obtained by separating from field environment is subjected to 18S rDNA sequence analysis and morphological identification and is determined as a member of monoraphidium, and the sequence is shown as SEQ NO.1. The grease-producing monoraphidium LB50 is capable of bearing high-concentration sodium bicarbonate, is easy for open-type culture and is rich in grease; and when the growth and collection speed is kept at 22.2 g / m<2>*d, the grease content reaches 30.4% by dry weight of a cell, and aliphatic acid contained in grease contains octadecatrienoic acid and octadecatetraenoic acid which account 15% or more of aliphatic acid.

Description

technical field [0001] The invention relates to the screening and cultivation technology of oleaginous microalgae, in particular to the acquisition and application of a monospina oleaginum LB50. Specifically, the present invention collects water samples from the field, and obtains an oleaginous algal strain that can be used as a raw material for oil production through separation and purification. Background technique [0002] There are more than 40,000 species of algae identified on Earth, some of which are rich in oil. The oil extracted from oil algae can be converted into biodiesel through reaction with methanol, which is considered the most likely to replace the depleted petroleum source of diesel. The 16-carbon and 18-carbon fatty acids it contains are most beneficial for biodiesel production. Algal oils also contain unsaturated fatty acids that can be added to animal feed to promote growth. Screening and obtaining algal species that are easy to cultivate, grow fast,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C11B1/00B01D53/84B01D53/62A23K1/16C12R1/89
CPCY02P20/151Y02P20/59Y02A50/20
Inventor 孔任秋徐旭东席玮芳高宏方仙桃
Owner INST OF AQUATIC LIFE ACAD SINICA
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