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A method for producing microalgae oil by using flue gas

A technology of microalgae oil and flue gas, which is applied in the fields of biotechnology and bioenergy, can solve the problems of not involving oil content, affecting the growth of algae cells, and bacterial contamination, so as to alleviate the greenhouse effect and exhaust gas pollution, and improve the fixation efficiency , the effect of promoting rapid growth

Active Publication Date: 2019-12-13
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
[0005] Liu Pinghuai et al. (The Effect of Organic Carbon Sources on Cell Growth, Oil Accumulation and Photosynthesis of Monospina, Bioengineering, 2012, 33(18): 224-246) introduced a production method of using organic carbon sources to cultivate Monospina , although the biomass exceeds 10g / L at the end of the culture, this method is a heterotrophic culture method of Monostipella. During the culture process, organic carbon sources such as glucose are used to achieve cell growth. This culture method does not use CO 2 Other inorganic carbon sources are economical, and the addition of organic carbon sources is prone to bacterial contamination during the cultivation process, which affects the growth of algae cells

Method used

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  • A method for producing microalgae oil by using flue gas
  • A method for producing microalgae oil by using flue gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) In a 10L photobioreactor, add Pseudochlorella kescheria FSH-Y3 seed solution and microalgae medium prepared in Example 1, the amount of seed solution added is 400mL, and the pH value of the microalgae medium is adjusted 10, the addition amount is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and CO 2 The content is 3v%, NO and NO 2 Content is 50×10 -6 (v / v), SO 2 Content is 60×10 -6 (v / v).

[0031] (2) After 4 days of cultivation, insert 400 mL of the Scenedesmus MH-04 seed liquid prepared in Example 1 and 400 mL of the cellulosic algae SS-B7 seed liquid, adjust the pH of the microalgae culture system to 8, and cultivate continuously under light at a light intensity of 5000Lux; into the CO in the flue gas 2 The content is 30v%, NO and NO 2 The content is 500×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0032] (3) After 7 days of culture, enter the stable growth period, end the culture...

Embodiment 2

[0034] (1) In a 10L photobioreactor, add the pseudo-Kjeldahl FSH-Y3 seed solution prepared in Example 1 and the microalgae medium, the amount of the seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 12. The amount added is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v), SO 2 The content is 100×10 -6 (v / v).

[0035] (2) After 2 days of cultivation, insert 560 mL of the Scenedesmus MH-04 seed liquid prepared in Example 1 and 560 mL of the cellulosic algae SS-B7 seed liquid, adjust the pH of the microalgae culture system to 9, and cultivate continuously under light at a light intensity of 5000Lux; into the CO in the flue gas 2 The content is 30v%, NO and NO 2 The content is 500×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0036] (3) After cultivating for 8 day...

Embodiment 3

[0038] (1) In a 10L photobioreactor, add the pseudo-Kjeldahl FSH-Y3 seed solution prepared in Example 1 and the microalgae medium, the amount of the seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 12. The amount added is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v), SO 2 The content is 100×10 -6 (v / v).

[0039] (2) After culturing for 2 days, insert 560 mL of the seed liquid of Monopodella SS-B1 prepared in Example 1 and 560 mL of the seed liquid of Cellulosa SS-B7, adjust the pH of the microalgae culture system to 9, continue the light culture, and the light intensity 5000Lux; through the CO in the flue gas 2 The content is 30v%, NO and NO 2 The content is 500×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0040] (3) After cultivating for 8 days, it ente...

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Abstract

The invention discloses a method for producing microalgae lipid by virtue of flue gas. The method comprises the following steps: (1) adding a microalgae medium and a parachlorella kessleri FSH-Y3 seed solution to a photobioreactor, regulating pH value of a culture system to 10-12, supplying the flue gas that a CO2 volume content is 1-5v%, and conducting culture for 2-5 days; and (2) regulating the pH value of the culture system to 8-10, inoculating the culture system with either a desmodesmus sp. MH-04 seed solution or a monoraphidium sp. SS-B1 seed solution or both of them, simultaneously, inoculating the culture system with an ankistrodesmus sp. SS-B7 and implementing mixed culture, and supplying the flue gas that a CO2 volume content is 5-45v%; implementing culture to a stationary phase under a continuous illumination condition, and collecting microalgae cells. According to the method provided by the invention, the tolerance and the solubility of the microalgae culture system to high-concentration CO2 are improved and a carbon fixation efficiency is improved, meanwhile, tolerance to SOx and NOx in the flue gas is improved, and the yield of the microalgae lipid is obviously improved.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for producing microalgae oil by using flue gas. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCB01D53/84B01D2257/504B01D2258/0283C12P7/6463Y02A50/20Y02P20/59
Inventor 师文静王鹏翔廖莎孙启梅李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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