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A method for preparing microalgae oil

A technology of microalgae oil and microalgae, which is applied in the fields of biotechnology and bioenergy, can solve the problems of unfavorable algae absorption and utilization, non-utilization, and affecting the growth of algae cells, so as to alleviate the problems of greenhouse effect and exhaust gas pollution, and improve tolerance and carbon fixation efficiency, the effect of inhibiting the growth of miscellaneous bacteria

Active Publication Date: 2020-04-14
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
The above patents either cannot efficiently utilize CO 2 Production of oils or fats, or obtaining biomass with insufficient oil content
Especially in practical applications, when the environment CO 2 When the volume fraction is greater than 5%, the growth of most microalgae will be inhibited, which will affect the carbon sequestration efficiency
At the same time, general microalgae are suitable for growth under neutral conditions, and it is not conducive to the growth of microalgae under acidic or alkaline conditions, while CO 2 Low solubility in neutral environment, not conducive to algae absorption and utilization
[0005] Liu Pinghuai et al. (The Effect of Organic Carbon Sources on Cell Growth, Oil Accumulation and Photosynthesis of Monospina, Bioengineering, 2012, 33(18): 224-246) introduced a production method of using organic carbon sources to cultivate Monospina , although the biomass exceeds 10g / L at the end of the culture, this method is a heterotrophic culture method of Monostipella. During the culture process, organic carbon sources such as glucose are used to achieve cell growth. This culture method does not use CO 2 Other inorganic carbon sources are economical, and the addition of organic carbon sources is prone to bacterial contamination during the cultivation process, which affects the growth of algae cells

Method used

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  • A method for preparing microalgae oil
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  • A method for preparing microalgae oil

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1 Preparation of Microalgae Seed Liquid

[0020] BG11 medium was used for microalgae culture, and the medium formula is shown in Table 1 and Table 2.

[0021] Table 1 BG11 medium

[0022]

[0023] *Table 2 Composition of A5+Co solution in Table 1

[0024]

[0025] Scenedesmus obliquus FSH-Y2 and Single needle sp. SS-B1 were respectively inoculated into BG11 liquid culture medium, and the pH of the culture medium for culturing Scenedesmus obliquus FSH-Y2 was adjusted to 10-12. Culture in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, and the culture is shaken at 120rpm to the logarithmic growth phase to obtain Scenedesmus obliquus FSH-Y2 The seed solution and the S. chinensis SS-B1 seed solution were stored under low light at 15°C for later use.

[0026] The above-mentioned FSH-Y2 seed liquid of Scenedesmus obliquus, SS-B1 seed liquid of Sin...

Embodiment 2

[0027] Example 2 Preparation of Microalgae Oil

[0028] The different seed solutions prepared in Example 1 were inoculated into BG11 medium respectively to prepare microalgal oil. In a photobioreactor, FSH-Y2 seed solution of Scenedesmus obliquus, SS-B1 seed solution of Single needle algae and mixed microalgae seed solution mixed in a volume ratio of 1:1 were inoculated into BG11 according to the inoculum amount of 10v% In the medium, the pH value is controlled between 9 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, wherein the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the culture temperature is 28°C, the light period is 24h, and the light The dark time ratio is 14:10. After culturing for 7 days, it was in a stable phase, and the culture was completed. The algal liquid was collected by centrifugation, and the dry weight of the algal powder was measured after vacuum freeze drying to constant weight under -60 ° C. The biomass...

Embodiment 3

[0032] Example 3 Preparation of Microalgae Oil by Using Flue Gas

[0033] The preparation conditions are the same as in Example 1, except that the feed containing SO 2 and CO 2 flue gas, CO in flue gas 2 The content is 10v% ~ 30v%, SO 2 The content is 200×10 -6 ~400×10 -6 (v / v), the pH of the reaction system was maintained at 10. The culture was terminated after 10 days, and the algal cells were collected by centrifugation. After vacuum freeze drying to constant weight at -60 °C, the dry weight of the algal powder was measured, and the biomass yield was calculated. Normal hexane: ethyl acetate method measures total lipid content, and the results are shown in Table 4.

[0034] Table 4 Comparison of culture effects between mixed algae and single algae

[0035]

[0036] It can be seen from Table 4 that in the high pH system, compared with single algae, mixed algae can not only tolerate high concentrations of CO 2 , and can tolerate a certain concentration of SO 2 , a h...

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Abstract

The invention discloses a method for preparing microalgae oil. The method comprises the following steps: adding a microalgae culture medium and a mixed microalgae seed solution to a photobioreactor, keeping the pH value of a culture system to be 8-12 and introducing gas which contains 5-45v% of CO2 by volume, wherein the mixed microalgae includes scenedesmus obliqnus FSH-Y2 and monoraphidium sp SS-B1 which are preserved in China General Microbiological Culture Collection Center on September 11, 2012 and April 15, 2013 by preservation numbers of CGMCC No.6551 and CGMCC No.7479, respectively. By virtue of the method disclosed by the invention, the solubility and the tolerance of the microalgae culture system to the high-concentration CO2 are improved, a carbon sequestration efficiency is improved and the yield of the microalgae oil is obviously improved, and the obtained microalgae oil can be used for producing biodiesel.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a CO 2 Method for preparing microalgal oil. Background technique [0002] Due to the increasing reduction of fossil energy and the increase of the greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forest biomass, crops, aquatic plants, agricultural wastes, etc. Among many biomass energy sources, microalgae are important renewable resources. They have the characteristics of wide distribution, large biomass, high photosynthesis efficiency, strong environmental adaptability, short growth cycle and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/64C12R1/89
CPCY02E50/10
Inventor 师文静姚新武廖莎孙启梅樊亚超高大成王领民李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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